Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,211,652 C→T intergenic (+75/+28) icdC → / ← iraM pseudogene, isocitrate dehydrogenase C‑terminal gene fragment, idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion/RpoS stabilzer during Mg starvation, anti‑RssB factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,211,6520CT100.0% 88.2 / NA 28intergenic (+75/+28)icdC/iraMpseudogene, isocitrate dehydrogenase C‑terminal gene fragment, idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion/RpoS stabilzer during Mg starvation, anti‑RssB factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/15);  total (13/15)

GGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCTTTTGAGTA  >  NC_000913/1211521‑1211783
                                                                                                                                   |                                                                                                                                   
ggCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGa                                                                                                                              <  1:1188486/139‑1 (MQ=255)
 gcgcTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAg                                                                                                                             <  1:139900/139‑1 (MQ=255)
 gcgcTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAg                                                                                                                             >  1:421443/1‑139 (MQ=255)
           gctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTtata                                                                                                                   <  2:722015/139‑1 (MQ=255)
                 aTGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAg                                                                                                                             <  2:649450/123‑1 (MQ=255)
                 aTGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAg                                                                                                                             >  1:649450/1‑123 (MQ=255)
                    ttCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCa                                                                                                          <  2:421443/139‑1 (MQ=255)
                                   aGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCata                                                                                           <  1:580516/139‑1 (MQ=255)
                                     cGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAg                                                                                                                             >  1:916933/1‑103 (MQ=255)
                                     cGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAg                                                                                                                             <  2:916933/103‑1 (MQ=255)
                                       atcatcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATggg                                                                                       <  1:607262/139‑1 (MQ=255)
                                                             tcCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCa                                                                                                                           <  2:800532/81‑1 (MQ=255)
                                                             tcCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTAGATCGAGCa                                                                                                                           >  1:800532/1‑81 (MQ=255)
                                                                       aaaTGTTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGa                                                                                                  >  2:764101/1‑96 (MQ=255)
                                                                       aaaTATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGa                                                                                                  <  1:764101/96‑1 (MQ=255)
                                                                             tGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCa                                                                                                          <  1:1022753/82‑1 (MQ=255)
                                                                             tGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCa                                                                                                          >  2:1022753/1‑82 (MQ=255)
                                                                                    ggCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGTATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTAtt                                          >  2:505797/1‑139 (MQ=255)
                                                                                               cGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCat                                                                                            >  2:998146/1‑78 (MQ=255)
                                                                                               cGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCat                                                                                            <  1:998146/78‑1 (MQ=255)
                                                                                               cGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTcc                               >  2:1008466/1‑139 (MQ=255)
                                                                                               cGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTcc                               >  1:364500/1‑139 (MQ=255)
                                                                                                     ttgttTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATggg                                                                                       <  1:991110/77‑1 (MQ=255)
                                                                                                     ttgttTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATggg                                                                                       >  2:991110/1‑77 (MQ=255)
                                                                                                                       tattatTAATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCt                                     >  2:1294107/1‑109 (MQ=255)
                                                                                                                       tattatTAATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCt                                     <  1:1294107/109‑1 (MQ=255)
                                                                                                                           attaATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCTTTTGAGt   >  2:510239/1‑139 (MQ=255)
                                                                                                                            ttaATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCTTTTGAGTa  <  1:1008466/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                   
GGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCTTTTGAGTA  >  NC_000913/1211521‑1211783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: