Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,387,095	G→A	A126T (GCA→ACA)	tyrR →	aromatic amino acid biosynthesis and transport regulon transcriptional regulator, autorepressor, ATPase, phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,387,095	0	G	A	100.0%	160.5 / NA	50	A126T (GCA→ACA)	tyrR	aromatic amino acid biosynthesis and transport regulon transcriptional regulator, autorepressor, ATPase, phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/26);  total (24/26)

CGTTACTGGAGGCGTTGCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCGCACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACGCCTGTTTATCTTCAGGATGAAAATG  >  NC_000913/1386970‑1387230                                                                                                                              |                                                                                                                                        cgTTACTGGAGGCGTTGCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTaa                                                                                                                            >  1:1176873/1‑139 (MQ=255) cgTTACTGGAGGCGTTGCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTaa                                                                                                                            >  2:483946/1‑139 (MQ=255)  gTTACTGGAGGCGTTGCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAAc                                                                                                                           <  1:263140/139‑1 (MQ=255)    tACTGGAGGCGTTGCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACgg                                                                                                                         >  1:588793/1‑139 (MQ=255)       tGGAGGCGTTGCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCtt                                                                                                                      <  2:588793/139‑1 (MQ=255)              gTTGCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAAtttt                                                                                                               >  1:623858/1‑139 (MQ=255)               ttGCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAAttttt                                                                                                              >  1:991729/1‑139 (MQ=255)                     gAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACAATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGtt                                                                                                        <  2:623858/139‑1 (MQ=255)                            tgttctctGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGa                                                                                                 >  2:1253686/4‑139 (MQ=255)                            tgCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGa                                                                                                 >  1:162229/1‑139 (MQ=255)                                 tctGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCg                                                                                            >  2:541268/1‑139 (MQ=255)                                 tctGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCg                                                                                            <  1:1253686/139‑1 (MQ=255)                                        atatGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCa                                                                                     >  1:82028/1‑139 (MQ=255)                                             aaaaGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGAtt                                                                                <  2:1176873/139‑1 (MQ=255)                                                    aaGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATaa                                                                         <  1:478694/139‑1 (MQ=255)                                                     aGTGGATATGGCGAACCCGGTGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATaa                                                                         >  2:978932/1‑138 (MQ=255)                                                     aGTGGATATGGCGAACCCGGTGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATaa                                                                         <  1:978932/138‑1 (MQ=255)                                                         gATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGc                                                                    <  2:211259/139‑1 (MQ=255)                                                         gATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGc                                                                    <  1:749472/139‑1 (MQ=255)                                                           tatGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCAt                                                                  <  2:991729/139‑1 (MQ=255)                                                           tatGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCAt                                                                  <  1:541268/139‑1 (MQ=255)                                                            atGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATaa                                                                         <  1:943359/131‑1 (MQ=255)                                                            atGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGAGTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATaa                                                                         >  2:943359/1‑131 (MQ=255)                                                                 gAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCAt                                                                  <  1:484909/133‑1 (MQ=255)                                                                 gAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCAt                                                                  >  2:484909/1‑133 (MQ=255)                                                                 gAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGtt                                                            >  2:68434/1‑139 (MQ=255)                                                                   aCCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGa                                                                      >  1:175764/1‑127 (MQ=255)                                                                   aCCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGa                                                                      <  2:175764/127‑1 (MQ=255)                                                                   aCCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGttat                                                          >  2:183549/1‑139 (MQ=255)                                                                    cccGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGtt                                                                                                        >  1:723839/1‑92 (MQ=255)                                                                    cccGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGtt                                                                                                        <  2:723839/92‑1 (MQ=255)                                                                         cGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTgg                                                                                                      >  1:974380/1‑89 (MQ=255)                                                                         cGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTgg                                                                                                      <  2:974380/89‑1 (MQ=255)                                                                         cGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATgg                                                    <  2:162229/139‑1 (MQ=255)                                                                             cTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAg                                                >  1:1019404/1‑139 (MQ=255)                                                                                         tGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATg                                    >  1:217933/1‑139 (MQ=255)                                                                                         tGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATg                                    <  2:1136918/139‑1 (MQ=255)                                                                                                 aaTTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTAc                            <  2:217933/139‑1 (MQ=255)                                                                                                  aTTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACg                           <  2:25326/139‑1 (MQ=255)                                                                                                    tGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACGcc                         <  1:608339/139‑1 (MQ=255)                                                                                                           ccTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGt                                                                                                         <  2:678323/52‑1 (MQ=255)                                                                                                           ccTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGt                                                                                                         >  1:678323/1‑52 (MQ=255)                                                                                                           ccTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGaaa                                                                                               >  1:782104/1‑62 (MQ=255)                                                                                                           ccTGCGCAACCATACCGCCACACAATTGAATAACGGCTTTAATTTTTTACGTTGGCTGGaaa                                                                                               <  2:782104/62‑1 (MQ=255)                                                                                                             tGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACGCCTGTTTATCt                >  2:784702/1‑139 (MQ=255)                                                                                                              gcgcAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACGCCTGTTTATCtt               <  1:684460/139‑1 (MQ=255)                                                                                                                    ccATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCg                                                                                       >  1:764085/1‑61 (MQ=255)                                                                                                                    ccATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGCAAAGCGGACCg                                                                                       <  2:764085/61‑1 (MQ=255)                                                                                                                    ccATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTCGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACGCCTGTTTATCTTCAGGAt         >  2:931700/1‑139 (MQ=255)                                                                                                                      aTACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACGCCTGTTTATCTTCAGGATGa       <  1:483946/139‑1 (MQ=255)                                                                                                                           gccacACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACGCCTGTTTATCTTCAGGATGAAAATg  <  2:1019404/139‑1 (MQ=255)                                                                                                                              |                                                                                                                                        CGTTACTGGAGGCGTTGCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCGCACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACGCCTGTTTATCTTCAGGATGAAAATG  >  NC_000913/1386970‑1387230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑