Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,389,242 C→T Q105* (CAA→TAA)  ycjG → L‑Ala‑D/L‑Glu epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,389,2420CT100.0% 81.9 / NA 27Q105* (CAA→TAA) ycjGL‑Ala‑D/L‑Glu epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/11);  total (16/11)

ATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGAAA  >  NC_000913/1389113‑1389367
                                                                                                                                 |                                                                                                                             
aTTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCgctgg                                                                                                                      <  2:704715/139‑1 (MQ=255)
          tcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGAt                                                                                                            >  2:523266/1‑139 (MQ=255)
          tcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGAt                                                                                                            >  1:881758/1‑139 (MQ=255)
                             aGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgag                                                                                         <  1:74201/139‑1 (MQ=255)
                                gCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCTGAGACa                                                                                      >  2:373887/1‑139 (MQ=255)
                                gCTGACACGGGAGGACTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACa                                                                                      >  2:878463/1‑139 (MQ=255)
                                   gACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGtt                                                                                   >  1:656403/1‑139 (MQ=255)
                                    acacGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTa                                                                                  >  2:104957/1‑139 (MQ=255)
                                     cacGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCGCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAAGCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTAt                                                                                 <  1:878463/139‑1 (MQ=255)
                                                ttGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGa                                                                      >  1:814937/1‑139 (MQ=255)
                                                    aaaaaaTTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGt                                                                  >  2:462591/1‑139 (MQ=255)
                                                        aaTTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTc                                                              <  2:656403/139‑1 (MQ=255)
                                                          ttCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGAGCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCAt                                                            <  2:881758/139‑1 (MQ=255)
                                                           tctcCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATc                                                           >  2:795314/1‑139 (MQ=255)
                                                             tcCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCTGAGACAGTTATCACTGCACAGACGGTTGTCATCgg                                                         <  1:373887/139‑1 (MQ=255)
                                                               ccTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTa                                                       <  1:104957/139‑1 (MQ=255)
                                                                                     cgcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAg                                 <  1:20180/139‑1 (MQ=255)
                                                                                          gATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgag                                                                                         <  2:64270/78‑1 (MQ=255)
                                                                                          gATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgag                                                                                         >  1:64270/1‑78 (MQ=255)
                                                                                                    tgtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTg                  >  1:1213884/1‑139 (MQ=255)
                                                                                                    tgtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTg                  >  1:1049505/1‑139 (MQ=255)
                                                                                                      tgGGATCTGGCCGCGCGATGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTggc                >  2:728839/1‑139 (MQ=255)
                                                                                                      tgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACg                                                                                                  <  2:975626/57‑1 (MQ=255)
                                                                                                      tgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACg                                                                                                  >  1:975626/1‑57 (MQ=255)
                                                                                                              ggCCGCGCGAAGACAGCAGTAATCGCTGGGTGATTTGGTCGGCATAAAGCTTCCCGAGACAGTTATCACTGCACAGATGGTGTCCATAGGTACGCCTACCCATCTGGCCATGACTGCATCAACACTCTggcaggcctga        >  2:129779/1‑135 (MQ=255)
                                                                                                                 cgcgcgAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGcgcg     <  1:1028097/139‑1 (MQ=255)
                                                                                                                    gcgAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGaaa  >  1:737912/1‑139 (MQ=255)
                                                                                                                              cagtaATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgag                                                                                         <  2:613217/42‑1 (MQ=255)
                                                                                                                              cagtaATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgag                                                                                         >  2:210530/1‑42 (MQ=255)
                                                                                                                              cagtaATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgag                                                                                         >  1:613217/1‑42 (MQ=255)
                                                                                                                              cagtaATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgag                                                                                         <  1:210530/42‑1 (MQ=255)
                                                                                                                                 |                                                                                                                             
ATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGAAA  >  NC_000913/1389113‑1389367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: