Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 150,947 T→C T3A (ACT→GCT)  yadC ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913150,9470TC100.0% 226.2 / NA 62T3A (ACT→GCT) yadCputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/29);  total (33/29)

GGTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCA  >  NC_000913/150813‑151082
                                                                                                                                      |                                                                                                                                       
ggTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTc                                                                                                                                     >  1:171091/1‑139 (MQ=255)
  tCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCAt                                                                                                                                   >  2:272261/1‑139 (MQ=255)
  tCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCAt                                                                                                                                   <  1:806144/139‑1 (MQ=255)
         tgCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACt                                                                                                                            >  2:1235526/1‑139 (MQ=255)
            ggCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCa                                                                                                                         >  2:276010/1‑139 (MQ=255)
             gCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCaa                                                                                                                        <  2:145128/139‑1 (MQ=255)
               aGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATg                                                                                                                      <  2:739180/139‑1 (MQ=255)
               aGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATGTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATg                                                                                                                      <  1:1235526/139‑1 (MQ=255)
                   gAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTc                                                                                                                  >  1:795878/1‑139 (MQ=255)
                       ggCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTa                                                                                                              <  1:520094/139‑1 (MQ=255)
                                 gTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGt                                                                                                    <  2:443072/139‑1 (MQ=255)
                                  ttATCAACTCCTGCATTATTTCCCACAATCAAACTTGTATATGCAGTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTa                                                                                                              >  2:758093/1‑128 (MQ=255)
                                  ttATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTa                                                                                                              <  1:758093/128‑1 (MQ=255)
                                    aTCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTg                                                                                                 <  1:628371/139‑1 (MQ=255)
                                            cTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGttt                                                                                         >  1:271387/1‑139 (MQ=255)
                                            cTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGttt                                                                                         >  1:422941/1‑139 (MQ=255)
                                                        ccACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAtt                                                                             <  1:272261/139‑1 (MQ=255)
                                                         cacaATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTc                                                                            >  1:1188683/1‑139 (MQ=255)
                                                              tAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCAtttt                                                                                                                                >  1:321879/1‑82 (MQ=255)
                                                              tAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCAtttt                                                                                                                                <  2:321879/82‑1 (MQ=255)
                                                              tAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAt                                                                              <  2:675979/132‑1 (MQ=255)
                                                              tAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAt                                                                              >  1:675979/1‑132 (MQ=255)
                                                              tAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgtagt                                                                       >  1:1315232/1‑139 (MQ=255)
                                                                   cTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAata                                                                  <  2:422941/139‑1 (MQ=255)
                                                                     tGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATc                                                                <  1:276010/139‑1 (MQ=255)
                                                                      gTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGt                                                                                                                     <  2:951340/85‑1 (MQ=255)
                                                                      gTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGt                                                                                                                     >  1:951340/1‑85 (MQ=255)
                                                                      gTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATcc                                                               >  2:400429/1‑139 (MQ=255)
                                                                      gTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATcc                                                               >  1:1034743/1‑139 (MQ=255)
                                                                              actgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTATATATCCACTAacac                                                       >  2:509224/1‑139 (MQ=255)
                                                                               ctgactgTATTACGACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTc                                                                            <  1:795545/117‑1 (MQ=255)
                                                                               ctgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCGTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTc                                                                            >  2:795545/1‑117 (MQ=255)
                                                                                tgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACAt                                                     <  2:171091/139‑1 (MQ=255)
                                                                                 gactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTa                                                                               <  2:1150979/112‑1 (MQ=255)
                                                                                 gactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTa                                                                               >  1:1150979/1‑112 (MQ=255)
                                                                                       aTTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCAt                                              >  2:388772/1‑139 (MQ=255)
                                                                                       aTTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCAt                                              <  2:1188683/139‑1 (MQ=255)
                                                                                            aacaaGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGtt                                         >  2:136561/1‑139 (MQ=255)
                                                                                              caaGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTGTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACAt                                                     >  2:855039/1‑125 (MQ=255)
                                                                                              caaGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACAt                                                     <  1:855039/125‑1 (MQ=255)
                                                                                              caaGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGa                                       >  2:767728/1‑139 (MQ=255)
                                                                                                aGAATACAGTGCTATAAAAAGAATGTATCTGAAGATAGCTTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                     <  2:1046185/139‑1 (MQ=255)
                                                                                                aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATg                                                                                                                      >  1:728297/1‑58 (MQ=255)
                                                                                                aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATg                                                                                                                      <  2:728297/58‑1 (MQ=255)
                                                                                                aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCAc                                                             <  1:108771/115‑1 (MQ=255)
                                                                                                aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCAc                                                             >  2:108771/1‑115 (MQ=255)
                                                                                                aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                     >  1:456613/1‑139 (MQ=255)
                                                                                                  aaTACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAg                                   <  1:136561/139‑1 (MQ=255)
                                                                                                  aaTACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAg                                   <  1:400429/139‑1 (MQ=255)
                                                                                                         tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCtt                                                                                                                >  2:1208996/1‑55 (MQ=255)
                                                                                                         tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCtt                                                                                                                <  1:1208996/55‑1 (MQ=255)
                                                                                                         tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTgcgc                                                                                              <  2:648843/73‑1 (MQ=255)
                                                                                                         tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTgcgc                                                                                              >  1:648843/1‑73 (MQ=255)
                                                                                                           cTAAAAAAAGAATTTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTACTTTATAGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAACTATCCACTAACACATGGATCATAAGTTGATCAGATGTAacac                          >  2:600416/1‑139 (MQ=255)
                                                                                                                     aaTGTACCTGAAGATAGCCTTCATTTTTACTCCAATg                                                                                                                      <  2:1283165/37‑1 (MQ=255)
                                                                                                                     aaTGTACCTGAAGATAGCCTTCATTTTTACTCCAATg                                                                                                                      >  1:1283165/1‑37 (MQ=255)
                                                                                                                               aaGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTgc                                                                                                >  1:482123/1‑49 (MQ=255)
                                                                                                                               aaGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTgc                                                                                                <  2:482123/49‑1 (MQ=255)
                                                                                                                                 gATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgt                                                                          >  2:758322/1‑69 (MQ=255)
                                                                                                                                 gATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgt                                                                          <  1:758322/69‑1 (MQ=255)
                                                                                                                                 gATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGAAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACTCTCAATGCAGGATCGATTTTTTg    >  2:1137674/1‑139 (MQ=255)
                                                                                                                                   tAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTCTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCa  >  1:1040672/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                       
GGTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTTTGCA  >  NC_000913/150813‑151082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑