Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,459,052	G→A	*263* (TAG→TAA)	paaG →	1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,459,052	0	G	A	100.0%	145.6 / NA	46	*263* (TAG→TAA)	paaG	1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/28);  total (18/28)

GCGCTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCG  >  NC_000913/1458916‑1459181                                                                                                                                         |                                                                                                                                     gcgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑a                                                                                                                                 <  1:675424/139‑1 (MQ=255) gcgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑a                                                                                                                                 >  2:14842/1‑139 (MQ=255) gcgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑a                                                                                                                                 <  1:447131/139‑1 (MQ=255) gcgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑a                                                                                                                                 <  1:1253759/139‑1 (MQ=255)  cgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑at                                                                                                                                <  2:855888/139‑1 (MQ=255)     tGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑atgat                                                                                                                             <  1:14842/139‑1 (MQ=255)     tGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑atgat                                                                                                                             <  1:115585/139‑1 (MQ=255)       aaaCCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑Atgatga                                                                                                                           >  2:347827/1‑139 (MQ=255)         aCCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATa                                                                                                                         <  2:66882/139‑1 (MQ=255)                      aTACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑atg                                                                                                                               >  2:524560/1‑120 (MQ=255)                      aTACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑atg                                                                                                                               <  1:524560/120‑1 (MQ=255)                      aTACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACtg                                                                                                            >  2:609493/1‑139 (MQ=255)                             aCTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGt                                                                                                     >  1:976487/1‑139 (MQ=255)                                 gATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTg                                                                                                    >  1:281511/1‑136 (MQ=255)                                 gATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACGGTGGCAGTg                                                                                                    <  2:281511/136‑1 (MQ=255)                                      ggAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAg                                                                                            <  1:57078/139‑1 (MQ=255)                                        aaCGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCg                                                                                          <  1:1142050/139‑1 (MQ=255)                                         aCGTGACTATCTGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCgg                                                                                         <  1:852983/139‑1 (MQ=255)                                         aCGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCgg                                                                                         <  1:609879/139‑1 (MQ=255)                                            tGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCAc                                                                                      >  1:790258/1‑139 (MQ=255)                                             gACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCAcc                                                                                     >  1:598082/1‑139 (MQ=255)                                              aCTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCa                                                                                    <  2:231197/139‑1 (MQ=255)                                                        aCTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcagg                                                                          >  2:588160/1‑139 (MQ=255)                                                        aCTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcagg                                                                          <  2:723028/139‑1 (MQ=255)                                                           tGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCAt                                                                       <  1:609493/139‑1 (MQ=255)                                                            gCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATa                                                                                                                         >  1:1251281/1‑88 (MQ=255)                                                            gCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATa                                                                                                                         <  2:1251281/88‑1 (MQ=255)                                                                gacggaGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTg                                                                  <  2:1060291/139‑1 (MQ=255)                                                                          aGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtgctgc                                                        <  2:598082/139‑1 (MQ=255)                                                                                        gtgtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTgg                                                                                                         >  2:1204616/1‑76 (MQ=255)                                                                                        gtgtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTgg                                                                                                         <  1:1204616/76‑1 (MQ=255)                                                                                        gtgtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACAcc                                          <  1:898081/139‑1 (MQ=255)                                                                                           tCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAgg                                       <  2:790258/139‑1 (MQ=255)                                                                                               tGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATa                                                                                                                         <  1:477066/53‑1 (MQ=255)                                                                                               tGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATa                                                                                                                         >  2:477066/1‑53 (MQ=255)                                                                                                 cGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCAt                                                                                   >  1:385850/1‑89 (MQ=255)                                                                                                 cGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCAt                                                                                   <  2:385850/89‑1 (MQ=255)                                                                                                 cGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTAc                                 <  1:588160/139‑1 (MQ=255)                                                                                                  gTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGtttt                                   <  1:506133/139‑1 (MQ=255)                                                                                                   ttCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcaggc                                                                         <  2:184416/97‑1 (MQ=255)                                                                                                   ttCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcaggc                                                                         >  1:184416/1‑97 (MQ=255)                                                                                                        ggCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACtgt                                                                                                           <  1:1184571/58‑1 (MQ=255)                                                                                                        ggCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACtgt                                                                                                           >  2:1184571/1‑58 (MQ=255)                                                                                                              aCGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTc                    >  1:1137184/1‑139 (MQ=255)                                                                                                                   cACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTctgctg               >  1:775941/1‑139 (MQ=255)                                                                                                                                ggggAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCcg  >  2:640948/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                     GCGCTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCG  >  NC_000913/1458916‑1459181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑