Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	152,036	C→T	V66I (GTA→ATA)	yadL ←	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	152,036	0	C	T	100.0%	170.6 / NA	53	V66I (GTA→ATA)	yadL	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/31);  total (22/31)

CCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACG  >  NC_000913/151912‑152163                                                                                                                             |                                                                                                                                cccTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATcaa                                                                                                                   <  2:1120674/139‑1 (MQ=255)    tCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGTTTTCAGAAATATATATATCTCCAAAATcaacaa                                                                                                                >  2:675972/1‑139 (MQ=255)    tCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATcaacaa                                                                                                                >  2:388870/1‑139 (MQ=255)    tCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATcaacaa                                                                                                                <  2:270353/139‑1 (MQ=255)    tCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATcaacaa                                                                                                                >  1:985464/1‑139 (MQ=255)         tgtgGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           <  1:431477/139‑1 (MQ=255)         tgtgGCTCGCTTGGTTAATTTTATTTGCGCTTTTTGATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           <  2:985464/139‑1 (MQ=255)         tgtgGCTCGCTTGGTTAATTTTATTTGCGCTTTCTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCATAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           <  1:831582/139‑1 (MQ=255)             gCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCTCCAAAATCAACAACAGAAATGt                                                                                                       <  1:675972/139‑1 (MQ=255)                cGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCt                                                                                                    >  1:370336/1‑139 (MQ=255)                  cTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           <  2:593589/130‑1 (MQ=255)                  cTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           >  1:593589/1‑130 (MQ=255)                           ttttATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGGAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCa                                                                                         <  2:272332/139‑1 (MQ=255)                           ttttATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCa                                                                                         <  1:1234978/139‑1 (MQ=255)                                          tttATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCGTGATCAGAGTCATTTACTAATGTAGCg                                                                          >  2:755061/1‑139 (MQ=255)                                           ttATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCgg                                                                         <  2:370336/139‑1 (MQ=255)                                                 gggATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCagag                                                                                            >  1:59370/1‑114 (MQ=255)                                                 gggATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCagag                                                                                            <  2:59370/114‑1 (MQ=255)                                                   gATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTa                                                                 >  1:694781/1‑139 (MQ=255)                                                        ccGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATcaaca                                                                                                                 <  2:1229381/86‑1 (MQ=255)                                                        ccGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATcaaca                                                                                                                 >  1:1229381/1‑86 (MQ=255)                                                           cacaGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTc                                                         <  2:694781/139‑1 (MQ=255)                                                              aGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTAc                                                      <  2:17375/139‑1 (MQ=255)                                                                tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCt                                                        <  1:27941/135‑1 (MQ=255)                                                                tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCt                                                        >  2:27941/1‑135 (MQ=255)                                                                 cTTTGAATGTGAGTTTTAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCAACAAAATCAACAACAGAAATGTCCTTATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACagt                                                   <  2:1138944/139‑1 (MQ=255)                                                                          tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGAt                                                                                                 <  1:1296168/84‑1 (MQ=255)                                                                          tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGAt                                                                                                 >  2:1296168/1‑84 (MQ=255)                                                                          tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACt                                                                                   >  2:952783/1‑98 (MQ=255)                                                                          tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACt                                                                                   <  1:952783/98‑1 (MQ=255)                                                                          tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTaa                                                                                 >  1:427527/1‑100 (MQ=255)                                                                          tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTaa                                                                                 <  2:427527/100‑1 (MQ=255)                                                                          tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTAccc                                                              <  1:224109/119‑1 (MQ=255)                                                                          tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTAccc                                                              >  2:224109/1‑119 (MQ=255)                                                                          tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAAc                                          >  1:445241/1‑139 (MQ=255)                                                                          tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAAc                                          >  2:131299/1‑139 (MQ=255)                                                                          tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAAc                                          >  2:15347/1‑139 (MQ=255)                                                                             gTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt                                       <  1:943736/139‑1 (MQ=255)                                                                             gTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt                                       <  1:997339/139‑1 (MQ=255)                                                                             gTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt                                       <  1:755061/139‑1 (MQ=255)                                                                             gTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt                                       <  1:15347/139‑1 (MQ=255)                                                                                  aaTGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTc                                  <  2:675669/139‑1 (MQ=255)                                                                                     gTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCaa                               <  2:633165/139‑1 (MQ=255)                                                                                              ttGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATActgtctg                      >  2:995363/1‑139 (MQ=255)                                                                                                 gTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           >  2:420854/1‑51 (MQ=255)                                                                                                 gTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           <  1:420854/51‑1 (MQ=255)                                                                                                      ggCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTAcc                                                               >  2:1199523/1‑90 (MQ=255)                                                                                                      ggCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTAcc                                                               <  1:1199523/90‑1 (MQ=255)                                                                                                           tGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTa                                                                             >  2:1107752/1‑71 (MQ=255)                                                                                                           tGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTa                                                                             <  1:1107752/71‑1 (MQ=255)                                                                                                                tCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           <  2:1097838/36‑1 (MQ=37)                                                                                                                tCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           >  1:1097838/1‑36 (MQ=37)                                                                                                                  aGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACg  <  2:445241/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                CCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACG  >  NC_000913/151912‑152163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑