Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,467,921	C→T	*302* (TAG→TAA)	insD1 ←	IS2 transposase TnpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,467,921	0	C	T	100.0%	111.5 / NA	37	*302* (TAG→TAA)	insD1	IS2 transposase TnpB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/21);  total (16/21)

AATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTC  >  NC_000913/1467787‑1468043                                                                                                                                       |                                                                                                                            aaTTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTtata                                                                                                                         >  2:780230/1‑139 (MQ=255)  aTTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTatat                                                                                                                        <  2:1099889/139‑1 (MQ=255)           ggggTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACa                                                                                                               <  1:780230/139‑1 (MQ=255)            gggTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACAt                                                                                                              <  2:797982/139‑1 (MQ=255)                  gCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTa                                                                                                        <  1:730607/139‑1 (MQ=255)                  gCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTa                                                                                                        <  1:530770/139‑1 (MQ=255)                  gCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTa                                                                                                        >  1:494933/1‑139 (MQ=255)                       ggTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                   <  2:494933/139‑1 (MQ=255)                                 gTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTa                                                                                        >  1:1056520/1‑139 (MQ=255)                                           aaGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCt                                                                              >  2:1159108/1‑139 (MQ=255)                                                                gTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcg                                                         <  1:362181/139‑1 (MQ=255)                                                                gTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcg                                                         <  2:284986/139‑1 (MQ=255)                                                                            taACAATTCAGGATATTGACAGTTTTGAACATTATGGGACAAGAACCCTGGATTTGTCCTTATATCTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATAGTCCCGTGGCGAGCGATAACCCa                                             <  2:1273205/139‑1 (MQ=18)                                                                               cAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                          <  2:133416/139‑1 (MQ=255)                                                                               cAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                          <  1:269596/139‑1 (MQ=255)                                                                                       gATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTatat                                                                                                                        <  1:1208660/54‑1 (MQ=255)                                                                                       gATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTatat                                                                                                                        >  2:1208660/1‑54 (MQ=255)                                                                                        atatTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCg                                 <  2:302188/139‑1 (MQ=255)                                                                                            tGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTCCCCAAGATATTCCCGTGCCGAGCAAAAACCCAGCGCACTATGCGGATg                             >  2:498678/1‑139 (MQ=17)                                                                                              aCAGTTTTGAACATCATGGGAAAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGcc                           <  1:498678/139‑1 (MQ=255)                                                                                                aGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAt                         <  2:1056520/139‑1 (MQ=255)                                                                                                 gTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGcc                                                                           <  2:112523/88‑1 (MQ=255)                                                                                                 gTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGcc                                                                           >  1:112523/1‑88 (MQ=255)                                                                                                 gTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAtt                        >  1:412287/1‑139 (MQ=255)                                                                                                     tGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATACTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGtt                    >  1:783991/1‑139 (MQ=255)                                                                                                            catGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCg                                                                          <  1:1060017/78‑1 (MQ=255)                                                                                                            catGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCg                                                                          >  2:1060017/1‑78 (MQ=255)                                                                                                            catGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGc             >  1:293986/1‑139 (MQ=255)                                                                                                                 gACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTgg                                                           >  1:724196/1‑88 (MQ=255)                                                                                                                 gACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTgg                                                           <  2:724196/88‑1 (MQ=255)                                                                                                                 gACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATACGGATGCCATTCGTTATAATGCTCGaa        <  2:783991/139‑1 (MQ=255)                                                                                                                    aaGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGc     <  2:293986/139‑1 (MQ=25)                                                                                                                     aGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGcc    >  2:1257512/1‑139 (MQ=21)                                                                                                                      gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGcc    >  2:867137/1‑139 (MQ=255)                                                                                                                      gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATg                             <  1:620149/113‑1 (MQ=21)                                                                                                                      gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATg                             >  2:620149/1‑113 (MQ=21)                                                                                                                       aaCCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCctc  >  1:56890/1‑139 (MQ=18)                                                                                                                                       |                                                                                                                            AATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAA‑CCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTC  >  NC_000913/1467787‑1468043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑