Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,503,125 G→A *223* (TAG→TAA ydcL → lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,503,1250GA100.0% 90.3 / NA 29*223* (TAG→TAAydcLlipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/14);  total (15/14)

GGTTATCAAAGTCGTTCGTCAGGGCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAGTTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTGCTGTTGTTCAATGTATT  >  NC_000913/1502990‑1503249
                                                                                                                                       |                                                                                                                            
ggTTATCAAAGTCGTTCGTCAGGGCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTACTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTc                                                                                                                           >  2:782028/1‑139 (MQ=255)
 gTTATCAAAGTCGTTCGTCAGGGCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTcc                                                                                                                          >  2:882476/1‑139 (MQ=255)
 gTTATCAAAGTCGTTCGTCAGGGCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTcc                                                                                                                          >  1:661561/1‑139 (MQ=255)
                    aGGGCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGAt                                                                                                       <  2:315561/139‑1 (MQ=255)
                      ggCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTACTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATgg                                                                                                     <  1:782028/139‑1 (MQ=255)
                                     cTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGt                                                                                      >  2:1219123/1‑139 (MQ=255)
                                        aaTAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTgcg                                                                                   >  2:80727/1‑139 (MQ=255)
                                                    aCGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGGCGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAAcccc                                                                       <  1:345198/139‑1 (MQ=255)
                                                          aTGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGCTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCtt                                                                 <  2:778412/139‑1 (MQ=255)
                                                               tttCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAggg                                                            <  2:470694/139‑1 (MQ=255)
                                                                 tCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCg                                                          <  1:1101750/139‑1 (MQ=255)
                                                                               cAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGgcgc                                            <  1:1219123/139‑1 (MQ=255)
                                                                                          tgaCATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAAACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCAcc                                 >  2:809055/1‑139 (MQ=255)
                                                                                            aCATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTg                               >  1:404677/1‑139 (MQ=255)
                                                                                             cATGGCGACCGATGTCACCATGTTTGACGTTAACAAAAAGTAATTCCAGAcgcg                                                                                                                   <  2:866378/54‑1 (MQ=255)
                                                                                             cATGGCGACCGATGCCACTATGTTTGACGTTAACAAAAAGTAATTCCAGAcgcg                                                                                                                   >  1:866378/1‑54 (MQ=255)
                                                                                              aTGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGAc                             <  1:80727/139‑1 (MQ=255)
                                                                                                ggCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCa                                                                                                                >  2:240477/1‑54 (MQ=255)
                                                                                                ggCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCa                                                                                                                <  1:240477/54‑1 (MQ=255)
                                                                                                ggCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGgc                                                                                                    <  1:585290/66‑1 (MQ=255)
                                                                                                ggCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGgc                                                                                                    >  2:585290/1‑66 (MQ=255)
                                                                                                ggCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCa                           >  2:796250/1‑139 (MQ=255)
                                                                                                          gCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCtt                                                                                                >  1:541133/1‑60 (MQ=255)
                                                                                                          gCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCtt                                                                                                <  2:541133/60‑1 (MQ=255)
                                                                                                                         gTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTGCTGTTGTTCAATGTAtt  >  2:46474/1‑139 (MQ=255)
                                                                                                                            aaCAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCa                                                                      <  2:254266/68‑1 (MQ=255)
                                                                                                                            aaCAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCa                                                                      >  1:254266/1‑68 (MQ=255)
                                                                                                                             aCAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGgcgc                                            <  1:1260619/93‑1 (MQ=255)
                                                                                                                             aCAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGgcgc                                            >  2:1260619/1‑93 (MQ=255)
                                                                                                                                   agtaatTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGtt                                        <  2:875331/91‑1 (MQ=255)
                                                                                                                                   agtaatTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGtt                                        >  1:875331/1‑91 (MQ=255)
                                                                                                                                       |                                                                                                                            
GGTTATCAAAGTCGTTCGTCAGGGCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAGTTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTGCTGTTGTTCAATGTATT  >  NC_000913/1502990‑1503249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: