Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,565,758	C→T	*461* (TAG→TAA)	dosC ←	diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,565,758	0	C	T	100.0%	138.8 / NA	43	*461* (TAG→TAA)	dosC	diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/23);  total (20/23)

AACATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAA  >  NC_000913/1565627‑1565888                                                                                                                                    |                                                                                                                                    gagacagcTTCATCATTTTCGGTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAc                                                                                                                              <  1:176580/132‑1 (MQ=255)                   tCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCTTTATCCCCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGC‑CATTTTAAGGCTTGGCTTTCCagttgtctc                                                                                                           >  2:176580/1‑132 (MQ=255)                   tCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCaa                                                                                                           >  1:113473/1‑139 (MQ=255)                                 cGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCt                                                                                             >  2:160010/1‑139 (MQ=255)                                   cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCg                                                                                           >  1:1038713/1‑139 (MQ=255)                                   cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCg                                                                                           >  1:20220/1‑139 (MQ=255)                                   cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCg                                                                                           >  1:1160247/1‑139 (MQ=255)                                    aCCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCagag                                                                                                                <  1:344310/117‑1 (MQ=255)                                    aCCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCagag                                                                                                                >  2:344310/1‑117 (MQ=255)                                        atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                      <  2:113473/139‑1 (MQ=255)                                             atTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTGGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCg                                                                                 <  1:160010/139‑1 (MQ=255)                                                  gCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGAtata                                                                            >  2:1294649/1‑139 (MQ=255)                                                  gCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGAtata                                                                            >  2:897146/1‑139 (MQ=255)                                                   cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAaca                                                                                                         >  2:1164194/1‑109 (MQ=255)                                                   cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAaca                                                                                                         <  1:1164194/109‑1 (MQ=255)                                                   cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATAc                                                                           >  1:153903/1‑139 (MQ=255)                                                                aaaaaaaTGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                              <  1:1294649/139‑1 (MQ=255)                                                                aaaaaaaTGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                              <  2:518319/139‑1 (MQ=255)                                                                        gCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCg                                                                                           <  1:906270/102‑1 (MQ=255)                                                                        gCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCg                                                                                           >  2:906270/1‑102 (MQ=255)                                                                             cggcggCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAg                                                 >  2:695147/1‑139 (MQ=255)                                                                              ggcggcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                <  2:1160247/139‑1 (MQ=255)                                                                                cggcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGgcg                                              <  1:897146/139‑1 (MQ=255)                                                                                  gcATTATCCGCATCGGTTAGCTTCCTGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCt                                            <  1:99617/139‑1 (MQ=255)                                                                                  gcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCt                                            <  1:714671/139‑1 (MQ=255)                                                                                  gcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCt                                            <  1:695147/139‑1 (MQ=255)                                                                                       aTCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAg                                       <  2:561754/139‑1 (MQ=255)                                                                                             aTCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGa                                 >  1:236751/1‑139 (MQ=255)                                                                                              tCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                              >  2:907358/1‑109 (MQ=255)                                                                                              tCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                              <  1:907358/109‑1 (MQ=255)                                                                                              tCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGAt                                <  2:261744/139‑1 (MQ=255)                                                                                              tCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGAt                                <  2:1038713/139‑1 (MQ=255)                                                                                                 gTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGAc                             <  2:20220/139‑1 (MQ=255)                                                                                                         cATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAAc                     <  2:153903/139‑1 (MQ=255)                                                                                                           tGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTg                                                                                    >  2:1269923/1‑74 (MQ=255)                                                                                                           tGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTg                                                                                    <  1:1269923/74‑1 (MQ=255)                                                                                                                aCCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                              >  2:814729/1‑91 (MQ=255)                                                                                                                aCCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGCCTTTTGGCGATATACAGAGCTTCATCgg                                                              <  1:814729/91‑1 (MQ=255)                                                                                                                      gCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGa        <  1:808502/139‑1 (MQ=255)                                                                                                                       cATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                      >  2:301871/1‑60 (MQ=255)                                                                                                                       cATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                      <  1:301871/60‑1 (MQ=255)                                                                                                                       cATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGAt       >  1:1081607/1‑139 (MQ=255)                                                                                                                            tGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGaaa  >  2:157610/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                    AACATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAA  >  NC_000913/1565627‑1565888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑