Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,651,537 G→A pseudogene (693/693 nt) insD1 → IS2 transposase TnpB,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,651,5370GA100.0% 103.0 / NA 33pseudogene (693/693 nt)insD1IS2 transposase TnpB,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/15);  total (18/15)

AGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAAC  >  NC_000913/1651425‑1651666
                                                                                                                |                                                                                                                                 
aGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGct                                                                                                         <  1:98266/139‑1 (MQ=255)
 gCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGctc                                                                                                        >  2:267949/1‑139 (MQ=255)
            aaTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCtgtttgtt                                                                                             >  1:409546/1‑139 (MQ=255)
              tGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATACACAGGTGAACTACGCTCCTCTGTTTGTTAc                                                                                           >  2:742084/1‑139 (MQ=255)
               ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGaa                                                                                                                <  1:552826/117‑1 (MQ=37)
               ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGaa                                                                                                                >  2:552826/1‑117 (MQ=37)
                   tCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAAt                                                                                      >  2:998136/1‑139 (MQ=255)
                              cgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGctcc                                                                                                       >  2:714234/1‑111 (MQ=255)
                              cgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGctcc                                                                                                       <  1:714234/111‑1 (MQ=255)
                                      tATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGa                                                                    >  1:50252/1‑138 (MQ=255)
                                      tATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGa                                                                    <  2:50252/138‑1 (MQ=255)
                                        tcgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTa                                                                 >  2:875664/1‑139 (MQ=255)
                                             cgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGct                                                                                                         >  2:788926/1‑94 (MQ=255)
                                             cgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGct                                                                                                         <  1:788926/94‑1 (MQ=255)
                                                                        cTTGTAATGGGTTAAGTGATAACAGGTGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGctcct                                                                                                      <  2:930662/70‑1 (MQ=18)
                                                                        cTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGctcct                                                                                                      >  1:930662/1‑70 (MQ=255)
                                                                                 ggTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTg                        >  1:904660/1‑139 (MQ=255)
                                                                                   ttAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGt                      <  1:720232/139‑1 (MQ=255)
                                                                                      aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGa                                <  1:184533/126‑1 (MQ=255)
                                                                                      aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGa                                >  2:184533/1‑126 (MQ=255)
                                                                                      aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAg                   >  1:1050306/1‑139 (MQ=255)
                                                                                           tAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCg              >  1:521433/1‑139 (MQ=255)
                                                                                            aaCAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGa             <  1:998136/139‑1 (MQ=255)
                                                                                             aCAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGAc            <  1:742084/139‑1 (MQ=255)
                                                                                                gATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCctct                                                                                                    >  1:271021/1‑48 (MQ=255)
                                                                                                gATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCctct                                                                                                    <  2:271021/48‑1 (MQ=255)
                                                                                                  tGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGAt       >  1:1078801/1‑139 (MQ=255)
                                                                                                    tCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGc                 <  2:1223962/127‑1 (MQ=255)
                                                                                                    tCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGc                 >  1:1223962/1‑127 (MQ=255)
                                                                                                       ggAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAAc  <  1:875664/139‑1 (MQ=255)
                                                                                                       ggAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAAc  <  2:1078801/139‑1 (MQ=255)
                                                                                                        gAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGa                                                                    <  2:445328/72‑1 (MQ=255)
                                                                                                        gAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGa                                                                    >  1:445328/1‑72 (MQ=255)
                                                                                                                |                                                                                                                                 
AGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAAC  >  NC_000913/1651425‑1651666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: