Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,655,057	T→C	T29A (ACG→GCG)	rspA ←	bifunctional D‑altronate/D‑mannonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,655,057	0	T	C	100.0%	144.7 / NA	41	T29A (ACG→GCG)	rspA	bifunctional D‑altronate/D‑mannonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/20);  total (21/20)

ACCTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAA  >  NC_000913/1654923‑1655187                                                                                                                                       |                                                                                                                                   aCCTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATa                                                                                                                                <  1:1115405/139‑1 (MQ=255)          gAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCa                                                                                                                       >  2:852332/1‑139 (MQ=255)                                      tGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTAc                                                                                           >  1:56323/1‑139 (MQ=255)                                        cgcATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGc                                                                                         >  1:1115431/1‑139 (MQ=255)                                                gACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACa                                                                                 >  1:575508/1‑139 (MQ=255)                                                                  aaaGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAgtggt                                                                                                                  <  1:29391/88‑1 (MQ=255)                                                                  aaaGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAgtggt                                                                                                                  >  2:29391/1‑88 (MQ=255)                                                                    aGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCa                                                                                                                       >  2:602891/1‑81 (MQ=255)                                                                    aGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCa                                                                                                                       <  1:602891/81‑1 (MQ=255)                                                                     ggTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCtt                                                            >  1:1127012/1‑139 (MQ=255)                                                                       tGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTa                                                          <  2:1094354/139‑1 (MQ=255)                                                                       tGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTa                                                          <  2:621221/139‑1 (MQ=255)                                                                       tGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTa                                                          <  1:852332/139‑1 (MQ=255)                                                                         aTCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACg                                                        >  2:418056/1‑139 (MQ=255)                                                                         aTCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACg                                                        <  1:418056/139‑1 (MQ=255)                                                                                aaaTAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGa                                                                                   <  2:393745/105‑1 (MQ=255)                                                                                aaaTAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGa                                                                                   >  1:393745/1‑105 (MQ=255)                                                                                aaaTAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCa                                                 >  1:723098/1‑139 (MQ=255)                                                                                         gCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACg                                                        <  2:698950/123‑1 (MQ=255)                                                                                         gCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACg                                                        >  1:698950/1‑123 (MQ=255)                                                                                         gCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTAAGCCTTTACGATCTTCATGTTCGAtt                                        >  2:874286/1‑139 (MQ=255)                                                                                           cACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAg                                                                                                                      >  1:1809/1‑59 (MQ=255)                                                                                           cACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAg                                                                                                                      <  2:1809/59‑1 (MQ=255)                                                                                           cACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGa                                                                                   <  1:30353/94‑1 (MQ=255)                                                                                           cACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGa                                                                                   >  2:30353/1‑94 (MQ=255)                                                                                             cGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCtt                                    >  2:1156621/1‑139 (MQ=255)                                                                                                             aTTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGa                    <  2:575508/139‑1 (MQ=255)                                                                                                             aTTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGa                    <  1:874286/139‑1 (MQ=255)                                                                                                                 aGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACGTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCa                <  2:29198/139‑1 (MQ=255)                                                                                                                   ggTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCa                <  2:703099/137‑1 (MQ=255)                                                                                                                   ggTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCa                >  1:703099/1‑137 (MQ=255)                                                                                                                   ggTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATg              >  2:1281085/1‑139 (MQ=255)                                                                                                                      ggCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGc                                  <  2:23105/116‑1 (MQ=255)                                                                                                                      ggCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGc                                  >  1:23105/1‑116 (MQ=255)                                                                                                                       gCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCg                              >  1:933667/1‑119 (MQ=255)                                                                                                                       gCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCg                              <  2:933667/119‑1 (MQ=255)                                                                                                                           ccccAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTAc                                                         >  2:443535/1‑88 (MQ=255)                                                                                                                           ccccAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTAc                                                         <  1:443535/88‑1 (MQ=255)                                                                                                                             ccAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACtt                                                                   >  1:911340/1‑76 (MQ=255)                                                                                                                             ccAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACtt                                                                   <  2:911340/76‑1 (MQ=255)                                                                                                                               aaGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCaa  <  2:208533/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                   ACCTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAA  >  NC_000913/1654923‑1655187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑