Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,789,804	G→A	*64* (TAG→TAA)	ydiE →	hemin uptake protein HemP homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,789,804	0	G	A	100.0%	129.9 / NA	42	*64* (TAG→TAA)	ydiE	hemin uptake protein HemP homolog
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/19);  total (23/19)

CTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAGCCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATC  >  NC_000913/1789671‑1789930                                                                                                                                      |                                                                                                                               cTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCttt                                                                                                                           >  1:1057613/1‑139 (MQ=255) cTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCttt                                                                                                                           >  2:76016/1‑139 (MQ=255) cTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCttt                                                                                                                           >  2:616868/1‑139 (MQ=255) cTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCttt                                                                                                                           >  2:440347/1‑139 (MQ=255)    ccccGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAAc                                                                                                                        >  1:454755/1‑139 (MQ=255)          aGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGagc                                                                                                                  <  2:454755/139‑1 (MQ=255)          aGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGagc                                                                                                                  <  2:1306345/139‑1 (MQ=255)             cTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGagcagc                                                                                                               <  2:684106/139‑1 (MQ=255)               aTTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTg                                                                                                             >  1:504872/1‑139 (MQ=255)                      gAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCa                                                                                                      <  1:616868/139‑1 (MQ=255)                       aaTTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAg                                                                                                     <  2:1057613/139‑1 (MQ=255)                         tttCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGcc                                                                                                   <  1:76016/139‑1 (MQ=255)                           tCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGt                                                                                                 <  2:504872/139‑1 (MQ=255)                                    acacTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAg                                                                                        >  1:266875/1‑139 (MQ=255)                                           tAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTg                                                                                 <  2:45384/139‑1 (MQ=255)                                                  ggATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGgacga                                                                            <  1:275646/137‑1 (MQ=255)                                                  ggATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGgacga                                                                            >  2:275646/1‑137 (MQ=255)                                                  ggATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACt                                                                          >  1:586557/1‑139 (MQ=255)                                                    aTGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGa                                                                        >  2:1239111/1‑139 (MQ=255)                                                      ggCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACa                                                                      <  2:588390/139‑1 (MQ=255)                                                                      aTCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACa                                                                      <  2:787304/123‑1 (MQ=255)                                                                      aTCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACa                                                                      >  1:787304/1‑123 (MQ=255)                                                                          tGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTg                                                                                 >  2:50230/1‑108 (MQ=255)                                                                          tGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTg                                                                                 <  1:50230/108‑1 (MQ=255)                                                                             cGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGaa                                               <  1:440347/139‑1 (MQ=255)                                                                                          cTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAAc                                  >  2:64502/1‑139 (MQ=255)                                                                                               ccGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTc                             >  2:632098/1‑139 (MQ=255)                                                                                                cGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAg                                                                                        >  1:1079999/1‑79 (MQ=255)                                                                                                cGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAg                                                                                        <  2:1079999/79‑1 (MQ=255)                                                                                                      aCTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAgg                      <  1:1051850/139‑1 (MQ=255)                                                                                                      aCTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAgg                      <  1:281754/139‑1 (MQ=255)                                                                                                          aGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTgg                                                                                <  1:608666/77‑1 (MQ=255)                                                                                                          aGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTgg                                                                                >  2:608666/1‑77 (MQ=255)                                                                                                          aGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGt                  >  1:1104309/1‑139 (MQ=255)                                                                                                            gCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAgg                      <  2:494907/133‑1 (MQ=255)                                                                                                            gCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAgg                      >  1:494907/1‑133 (MQ=255)                                                                                                            gCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCACAACGCCTCATGCAGGCGGTGc                >  2:576473/1‑139 (MQ=255)                                                                                                                gCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTg                                                                         <  1:1305288/78‑1 (MQ=255)                                                                                                                gCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTg                                                                         >  2:1305288/1‑78 (MQ=255)                                                                                                                      tgctgTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCa      >  1:342810/1‑139 (MQ=255)                                                                                                                          gTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATc  >  1:354756/1‑139 (MQ=255)                                                                                                                          gTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATc  >  1:354293/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                               CTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAGCCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATC  >  NC_000913/1789671‑1789930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑