Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,819,569 A→G G79G (GGT→GGC)  chbA ← N,N'‑diacetylchitobiose‑specific enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,819,5690AG100.0% 169.2 / NA 47G79G (GGT→GGC) chbAN,N'‑diacetylchitobiose‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/20);  total (27/20)

TCATCGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGC  >  NC_000913/1819444‑1819703
                                                                                                                             |                                                                                                                                      
tcatcGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                           <  1:1294531/139‑1 (MQ=255)
          cTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAAt                                                                                                                 >  1:963855/1‑139 (MQ=255)
             aTGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAg                                                                                                              >  2:239784/1‑139 (MQ=255)
                ccTTCAGTTTTTCATGAAGCGCAATTGATCCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCCGttt                                                                                                           >  2:915188/1‑139 (MQ=255)
                    cAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTc                                                                                                       >  2:655835/1‑139 (MQ=255)
                                        ttaattCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCttcatt                                                                                   <  1:202737/139‑1 (MQ=255)
                                        ttaaatCAGTTATTCGTTCACCCGCCAACATGGGCGTCATTAAATTATCCTGAGCCTGGACCAGCCCCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCCATCAGTTTCGGCTGTACCAGATGCGCttcatt                                                                                   <  2:106719/139‑1 (MQ=255)
                                                gTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGcct                                                                           >  1:106719/1‑138 (MQ=255)
                                                gTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCa                                                                           >  2:288862/1‑139 (MQ=255)
                                                 tAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCAt                                                                          >  2:59451/1‑139 (MQ=255)
                                                 tAATCAGATCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCAt                                                                          >  2:150597/1‑139 (MQ=255)
                                                  aaTCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATc                                                                         <  1:59451/139‑1 (MQ=255)
                                                       gTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGa                                                                    >  2:1159031/1‑139 (MQ=255)
                                                         tCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCACACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACt                                                                  >  2:760361/1‑139 (MQ=255)
                                                           aCGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATc                                                                         <  2:103770/130‑1 (MQ=255)
                                                           aCGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATc                                                                         >  1:103770/1‑130 (MQ=255)
                                                           aCGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGa                                                                >  2:834167/1‑139 (MQ=255)
                                                           aCGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGa                                                                <  1:655835/139‑1 (MQ=255)
                                                                cAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGAt                                                                                             <  2:681116/105‑1 (MQ=255)
                                                                cAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGAt                                                                                             >  1:681116/1‑105 (MQ=255)
                                                                            tCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTtgc                                               <  1:915188/139‑1 (MQ=255)
                                                                             cATTAAATGATGCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCg                                                                                                                         >  2:762778/1‑64 (MQ=255)
                                                                             cATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCg                                                                                                                         <  1:762778/64‑1 (MQ=255)
                                                                             cATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTtgct                                              <  1:520853/139‑1 (MQ=255)
                                                                             cATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTtgct                                              >  2:520853/1‑139 (MQ=255)
                                                                                       tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAAt                                    >  1:829583/1‑139 (MQ=255)
                                                                                        ccTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATc                                   >  1:147184/1‑139 (MQ=255)
                                                                                           gAGCGTGGACCAGCACCAGACGCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCGTCATGGCTTTTGCTGCGGCAAAATCGcc                                <  2:765194/139‑1 (MQ=255)
                                                                                               gTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGc                            <  1:239784/139‑1 (MQ=255)
                                                                                                tGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGc                            >  1:188921/1‑138 (MQ=255)
                                                                                                tGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGc                            <  2:188921/138‑1 (MQ=255)
                                                                                                 ggACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                           <  2:1125403/42‑1 (MQ=255)
                                                                                                 ggACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                           >  1:1125403/1‑42 (MQ=255)
                                                                                                 ggACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGAc                                                                   >  1:1068339/1‑98 (MQ=255)
                                                                                                 ggACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGAc                                                                   <  2:1068339/98‑1 (MQ=255)
                                                                                                 ggACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCtt                          >  1:433871/1‑139 (MQ=255)
                                                                                                      aGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGAt                                                                                             >  1:1128103/1‑67 (MQ=255)
                                                                                                      aGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGAt                                                                                             <  2:1128103/67‑1 (MQ=255)
                                                                                                      aGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATg                                                                              <  2:625743/82‑1 (MQ=255)
                                                                                                      aGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATg                                                                              >  1:625743/1‑82 (MQ=255)
                                                                                                      aGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCt                     >  1:34364/1‑139 (MQ=255)
                                                                                                             gACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGAc                                                                   <  1:1053104/86‑1 (MQ=255)
                                                                                                             gACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGAc                                                                   >  2:1053104/1‑86 (MQ=255)
                                                                                                              aCTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGt             <  1:288862/139‑1 (MQ=255)
                                                                                                                  aCTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTc                                                                                                       >  1:19776/1‑45 (MQ=255)
                                                                                                                  aCTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTc                                                                                                       <  2:19776/45‑1 (MQ=255)
                                                                                                                         tCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGc  >  1:404429/1‑139 (MQ=255)
                                                                                                                             |                                                                                                                                      
TCATCGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGC  >  NC_000913/1819444‑1819703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑