Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,834,111 G→A *237* (TAG→TAA)  ydjX → TVP38/TMEM64 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,834,1110GA100.0% 173.6 / NA 54*237* (TAG→TAA) ydjXTVP38/TMEM64 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/26);  total (28/26)

TATTTTACAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATACGACGGACT  >  NC_000913/1833976‑1834240
                                                                                                                                       |                                                                                                                                 
tATTTTACAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAAc                                                                                                                                <  1:396366/139‑1 (MQ=255)
       cAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg                                                                                                                         <  1:743338/139‑1 (MQ=255)
       cAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg                                                                                                                         <  2:399302/139‑1 (MQ=255)
          ctctGGCTGGAGTGCCTGGCGCTGTTTATTCTCGTCCACCCCGCAAAATTCTACGTCCGACTCAAACGCCACGATGTATCTTCTTCGCCCCTCAGCCTACATACTCACCCAAAAAATGAAGAATAAAACGATGTTGCac                                                                                                                      >  2:1301767/1‑138 (MQ=255)
            ctgtctgGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACTCAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCa                                                                                                                       <  2:942025/136‑1 (MQ=255)
            ctgtctgGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCa                                                                                                                       >  1:942025/1‑136 (MQ=255)
            ctgtctgGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACa                                                                                                                    >  2:344875/1‑139 (MQ=255)
                    cGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACGCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAg                                                                                                            >  2:829964/1‑139 (MQ=255)
                                 gTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACa                                                                                                                    <  2:329807/118‑1 (MQ=255)
                                 gTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACa                                                                                                                    >  1:329807/1‑118 (MQ=255)
                                  tttATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCa                                                                                                                       <  2:696632/114‑1 (MQ=255)
                                  tttATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCAACCAAAAAATGAAGGATAAAACGATGTTGCa                                                                                                                       >  1:696632/1‑114 (MQ=255)
                                     aTTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGa                                                                                           >  1:1044891/1‑139 (MQ=255)
                                           gTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCt                                                                                     >  2:192920/1‑139 (MQ=255)
                                            tCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATg                                                                                                     >  2:882520/1‑122 (MQ=255)
                                            tCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATg                                                                                                     <  1:882520/122‑1 (MQ=255)
                                            tCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTg                                                                                    >  1:682578/1‑139 (MQ=255)
                                              cAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCa                                                                                  <  1:192920/139‑1 (MQ=255)
                                               aGCTCGCAAAACTCTACGCACGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAACAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCAc                                                                                 <  2:860009/139‑1 (MQ=255)
                                                gCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAGTCGTTGTTGCAACATTATTCAGTGTTATGGAAAAAAGGACTGGCTGCAct                                                                                <  2:890598/139‑1 (MQ=255)
                                                gCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCAct                                                                                <  1:829964/139‑1 (MQ=255)
                                                 cTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg                                                                                                                         <  2:609180/97‑1 (MQ=255)
                                                 cTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg                                                                                                                         >  1:609180/1‑97 (MQ=255)
                                                            ctACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGc                                                                    <  2:1137112/139‑1 (MQ=255)
                                                               cGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGt                                                                 >  1:647905/1‑139 (MQ=255)
                                                                    gACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTg                                                            >  2:45060/1‑139 (MQ=255)
                                                                     acacAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCa                                                                                                                       >  2:661912/1‑79 (MQ=255)
                                                                     acacAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCa                                                                                                                       <  1:661912/79‑1 (MQ=255)
                                                                     acacAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCa                                                                                                             >  2:979156/1‑89 (MQ=255)
                                                                     acacAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCa                                                                                                             <  1:979156/89‑1 (MQ=255)
                                                                         aaaCATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCtt                                                       >  1:1215778/1‑139 (MQ=255)
                                                                                 ggATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACattatt                                                                                                               >  1:437708/1‑75 (MQ=255)
                                                                                 ggATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACattatt                                                                                                               <  2:437708/75‑1 (MQ=255)
                                                                                       gtctgCTTCTCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAGCATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATca                                         >  2:546075/1‑139 (MQ=255)
                                                                                       gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACa                                                                                                                    >  1:630080/1‑64 (MQ=255)
                                                                                       gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACa                                                                                                                    <  2:630080/64‑1 (MQ=255)
                                                                                       gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGc                                                                                   >  2:148481/1‑97 (MQ=255)
                                                                                       gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGc                                                                                   <  1:148481/97‑1 (MQ=255)
                                                                                       gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATca                                         >  1:1297843/1‑139 (MQ=255)
                                                                                       gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATca                                         >  1:85479/1‑139 (MQ=255)
                                                                                       gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATca                                         >  2:696610/1‑139 (MQ=255)
                                                                                        tctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg                                                                                                                         >  2:183920/1‑58 (MQ=255)
                                                                                        tctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg                                                                                                                         <  1:183920/58‑1 (MQ=255)
                                                                                        tctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATcaa                                        <  2:647905/139‑1 (MQ=255)
                                                                                                  gccgcAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCAc                                                                                 >  2:1015592/1‑88 (MQ=255)
                                                                                                  gccgcAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCAc                                                                                 <  1:1015592/88‑1 (MQ=255)
                                                                                                        gCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGcggcgg                        <  2:1215778/139‑1 (MQ=255)
                                                                                                           cACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCaa                     <  2:85479/139‑1 (MQ=255)
                                                                                                                      ccAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATac          >  1:81192/1‑139 (MQ=255)
                                                                                                                        aaaaaaTGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGAt                                           <  2:703774/104‑1 (MQ=255)
                                                                                                                        aaaaaaTGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGAt                                           >  1:703774/1‑104 (MQ=255)
                                                                                                                        aaaaaaTGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATacga        <  2:1044891/139‑1 (MQ=255)
                                                                                                                          aaaaTGAAGGATAAAACGATGTTGCAACATTTTTCCGGGTCATGGAAAAAAGGGCTGGCTGCACCCCGTTTAATGGCTGTTGCTGGGCTTTGCGGCTGCGATTAACAAAAGAATGCGGCGGCAAAAGTGGAATacgacg      <  1:480288/139‑1 (MQ=255)
                                                                                                                              tGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGGGGCAAAAGTGAATAAGGACGGACt  >  2:480288/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                 
TATTTTACAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATACGACGGACT  >  NC_000913/1833976‑1834240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑