Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,805	G→A	G40S (GGC→AGC)	mntP →	putative Mn(2+) efflux pump, mntR‑regulated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,805	0	G	A	100.0%	175.4 / NA	55	G40S (GGC→AGC)	mntP	putative Mn(2+) efflux pump, mntR‑regulated
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/25);  total (30/25)

CCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATG  >  NC_000913/1905670‑1905939                                                                                                                                        |                                                                                                                                         cccGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGcc                                                                                                                                       <  1:1008039/139‑1 (MQ=255)   cGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCtt                                                                                                                                     >  1:955394/1‑139 (MQ=255)      tCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTAtt                                                                                                                                  >  2:190740/1‑139 (MQ=255)           aCATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTgg                                                                                                                             >  1:420885/1‑139 (MQ=255)                    tGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGa                                                                                                                    >  2:788663/1‑139 (MQ=255)                          tCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCt                                                                                                              >  1:780227/1‑139 (MQ=255)                            aCTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGa                                                                                                            <  2:1118239/139‑1 (MQ=255)                                cTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgc                                                                                                         >  2:1256583/1‑138 (MQ=255)                                cTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgc                                                                                                         <  1:1256583/138‑1 (MQ=255)                                  aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgccg‑c                                                                                                     >  1:653003/1‑139 (MQ=255)                                      ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGAt                                                                                                 <  2:57184/139‑1 (MQ=255)                                      ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGAt                                                                                                 <  2:1139255/139‑1 (MQ=255)                                        cttcttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCg                                                                                               >  2:859231/1‑139 (MQ=255)                                          tcttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGc                                                                                             >  2:827502/1‑139 (MQ=255)                                           cttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCt                                                                                            >  2:850626/1‑139 (MQ=255)                                                        tGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCTTAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCAt                                                                               >  1:526843/1‑139 (MQ=255)                                                             atggatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTg                                                                                                   <  2:916470/114‑1 (MQ=255)                                                             atggatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTg                                                                                                   >  1:916470/1‑114 (MQ=255)                                                               ggatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTagcc                                                                        >  1:926712/1‑139 (MQ=255)                                                                 atgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTagccag                                                                      <  2:420885/139‑1 (MQ=255)                                                                    cATTTGCTGCGTCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCg                                                                   >  2:1303956/1‑139 (MQ=255)                                                                    cATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGAt                                                                                                 <  1:1138662/109‑1 (MQ=255)                                                                    cATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGAt                                                                                                 >  2:1138662/1‑109 (MQ=255)                                                                        tgctgcATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGAcg                                                                                                          >  1:307775/1‑97 (MQ=255)                                                                        tgctgcATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGAcg                                                                                                          <  2:307775/97‑1 (MQ=255)                                                                               tCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCtt                                                                                                                                     <  2:347138/63‑1 (MQ=255)                                                                               tCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCtt                                                                                                                                     >  1:347138/1‑63 (MQ=255)                                                                                 aaTCGGTAAAGGTGCCACCCTCCATAAACTGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGaa                                                     <  1:887584/139‑1 (MQ=255)                                                                                  aTCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGAAt                                                    <  2:653003/139‑1 (MQ=255)                                                                                             tGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGTTCCTGTC‑AAAACCTGGCCCCGCCGGCTCGCCT‑GGGTATGGGGCTGGTAGGCAACCCGTTTGTCCTTTAAATGAAACCCCTgg                                         >  2:245952/1‑139 (MQ=255)                                                                                             tGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGAATGGaa                                                <  1:477879/132‑1 (MQ=255)                                                                                             tGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGAATGGaa                                                >  2:477879/1‑132 (MQ=255)                                                                                             tGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGAATGGAACCACTgg                                         >  1:1185828/1‑139 (MQ=255)                                                                                               ccACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGAt                                                                                                 >  1:562827/1‑82 (MQ=255)                                                                                               ccACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGAt                                                                                                 <  2:562827/82‑1 (MQ=255)                                                                                               ccACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGAATGGAACCACTGGAt                                       >  2:1118752/1‑139 (MQ=255)                                                                                                      ccATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGAATGGaa                                                <  2:1209204/123‑1 (MQ=255)                                                                                                      ccATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGAATGGaa                                                >  1:1209204/1‑123 (MQ=255)                                                                                                        aTAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGAATGGAACCACTGGATTGCGTTtgt                              <  1:827502/139‑1 (MQ=255)                                                                                                            aCCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGAt                                                                                                 <  1:388829/69‑1 (MQ=255)                                                                                                            aCCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGAt                                                                                                 >  2:388829/1‑69 (MQ=255)                                                                                                            aCCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑t                                                         <  1:627997/108‑1 (MQ=255)                                                                                                            aCCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑t                                                         >  2:627997/1‑108 (MQ=255)                                                                                                            aCCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGAATGGAACCACTGGATTGCGTTTGtgctg                          >  1:286148/1‑139 (MQ=255)                                                                                                            aCCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGAATGGAACCACTGGATTGCGTTTGtgctg                          <  1:788663/139‑1 (MQ=255)                                                                                                               gAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTagccag                                                                      >  2:817786/1‑93 (MQ=255)                                                                                                               gAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTagccag                                                                      <  1:817786/93‑1 (MQ=255)                                                                                                                 aaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat                     >  2:268282/1‑139 (MQ=255)                                                                                                                                  cGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGaa    <  2:536247/139‑1 (MQ=255)                                                                                                                                  cGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGaa    >  2:1084861/1‑139 (MQ=255)                                                                                                                                   gAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAAt   <  1:859231/139‑1 (MQ=255)                                                                                                                                    aaCCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATg  <  2:631850/139‑1 (MQ=255)                                                                                                                                    aaCCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATg  <  2:526843/139‑1 (MQ=255)                                                                                                                                     aCCAGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgc                                                                                                         <  2:1118026/37‑1 (MQ=255)                                                                                                                                     aCCAGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgc                                                                                                         >  1:1118026/1‑37 (MQ=255)                                                                                                                                        |                                                                                                                                         CCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCG‑CTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCC‑TTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATG  >  NC_000913/1905670‑1905939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑