Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,068,952 C→T *302* (TAG→TAA insD1 ← IS2 transposase TnpB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,068,9520CT99.4% 1077.1 / ‑3.0 329*302* (TAG→TAAinsD1IS2 transposase TnpB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (159/168);  minor base A (1/1);  total (160/169)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 329 total aligned reads displayed.

TATTGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCC  >  NC_000913/2068817‑2069072
                                                                                                                                       |                                                                                                                        
tATTGACCAACAAACATTGCAGAACTCCACCAATGCCACCATAACCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGACCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTtat                                                                                                                       >  1:879086/1‑139 (MQ=255)
   tGATCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATGACAGACAACTCCTTGGCTGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATAtt                                                                                                                    >  2:846437/1‑139 (MQ=255)
   tGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATAtt                                                                                                                    >  1:104456/1‑139 (MQ=255)
   tGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATAtt                                                                                                                    >  1:1054970/1‑139 (MQ=255)
   tGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATAtt                                                                                                                    >  2:266985/1‑139 (MQ=255)
   tGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATAtt                                                                                                                    >  2:455376/1‑139 (MQ=255)
   tGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGAAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATAtt                                                                                                                    >  2:896610/1‑139 (MQ=255)
        aacaaacaTTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAg                                                                                                               >  2:962871/1‑139 (MQ=255)
           aaacaTTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACa                                                                                                            <  2:588983/139‑1 (MQ=255)
                       aCTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGACGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                <  1:267205/139‑1 (MQ=255)
                         tCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTa                                                                                              >  2:506223/1‑139 (MQ=255)
                          ccaccaATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCACATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTaa                                                                                             <  2:687900/139‑1 (MQ=255)
                           caccaATGCCTCCATAGTCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAc                                                                                            >  1:823068/1‑139 (MQ=255)
                           caccaATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAc                                                                                            >  2:1292085/1‑139 (MQ=255)
                             ccaATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                >  2:1303651/1‑133 (MQ=255)
                                 tGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTa                                                                                      >  2:554422/1‑139 (MQ=255)
                                     tCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAg                                                                                  <  1:1164297/139‑1 (MQ=255)
                                         tAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGcc                                                                                >  1:1248047/1‑137 (MQ=255)
                                         tAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                              <  2:1036862/139‑1 (MQ=255)
                                          aGCAGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGc                                                                             <  1:846437/139‑1 (MQ=255)
                                              gATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGcc                                                                         <  2:802877/139‑1 (MQ=255)
                                               aTAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCg                                                                        <  2:974767/139‑1 (MQ=255)
                                                tAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGc                                                                       >  2:1043541/1‑139 (MQ=255)
                                                   tttGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGa                                                                    <  1:506223/139‑1 (MQ=255)
                                                      gCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGcc                                                                         <  1:983604/131‑1 (MQ=255)
                                                           aCATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACa                                                                                                            <  1:337340/91‑1 (MQ=255)
                                                            cATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                           <  2:94650/107‑1 (MQ=255)
                                                            cATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                           >  1:94650/1‑107 (MQ=255)
                                                               ccccGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                           >  1:1129148/1‑104 (MQ=255)
                                                                cccGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcg                                                       <  1:631582/139‑1 (MQ=255)
                                                                     aGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATAttt                                                                                                                   <  2:1005515/74‑1 (MQ=255)
                                                                     aGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcgagcga                                                  <  2:1054970/139‑1 (MQ=255)
                                                                       tAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATa                                                <  2:1071610/139‑1 (MQ=255)
                                                                         gagaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTc                                                                                                                  >  2:659113/1‑71 (MQ=255)
                                                                         gagaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGAc                                                                                                             <  2:1174413/76‑1 (MQ=255)
                                                                         gagaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACa                                                                                                            >  2:169568/1‑77 (MQ=255)
                                                                         gagaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACAt                                                                                                           >  2:1025993/1‑78 (MQ=255)
                                                                         gagaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAAc                                              >  1:296503/1‑139 (MQ=255)
                                                                          agaTGACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGGCCTTATATTTCCAGACa                                                                                                            <  2:752968/76‑1 (MQ=255)
                                                                          agaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACa                                                                                                            >  1:752968/1‑76 (MQ=255)
                                                                          agaTAACAGACAACGCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAAACATCTGTTATCACtt                                                                                               >  1:799201/1‑89 (MQ=255)
                                                                               aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCa                                                                                                                <  2:320790/67‑1 (MQ=255)
                                                                               aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTaa                                                                                             >  1:285006/1‑86 (MQ=255)
                                                                               aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                              <  2:1274634/101‑1 (MQ=255)
                                                                               aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAAcc                                             >  1:1095756/1‑134 (MQ=255)
                                                                               aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAAcc                                             <  2:1095756/134‑1 (MQ=255)
                                                                               aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                        >  1:732871/1‑139 (MQ=255)
                                                                               aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                        >  1:1177030/1‑139 (MQ=255)
                                                                               aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                        <  2:444026/139‑1 (MQ=255)
                                                                               aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                        <  2:296503/139‑1 (MQ=255)
                                                                               aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                        <  1:208655/139‑1 (MQ=255)
                                                                                 aGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGc                                                                       <  2:771600/106‑1 (MQ=255)
                                                                                 aGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGc                                                                       >  1:771600/1‑106 (MQ=255)
                                                                                 aGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCa                                      <  1:1011315/139‑1 (MQ=255)
                                                                                   aCAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCa                                                                                                                >  1:1082979/1‑63 (MQ=255)
                                                                                   aCAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACa                                                                                                            >  2:1185493/1‑67 (MQ=255)
                                                                                   aCAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                              >  2:618282/1‑97 (MQ=255)
                                                                                   aCAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCg                                                                        >  2:1045963/1‑103 (MQ=255)
                                                                                   aCAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACt                                    >  2:609478/1‑139 (MQ=255)
                                                                                     aaCTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTaa                                                                                             <  1:320398/80‑1 (MQ=255)
                                                                                      aCTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATa                                                <  1:640506/124‑1 (MQ=255)
                                                                                      aCTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATa                                                >  2:640506/1‑124 (MQ=255)
                                                                                      aCTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                        <  1:1032913/132‑1 (MQ=255)
                                                                                       cTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGcc                                                                         <  1:1197745/98‑1 (MQ=255)
                                                                                       cTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGcc                                                                         >  2:1197745/1‑98 (MQ=255)
                                                                                        tCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCg                               <  1:533469/139‑1 (MQ=255)
                                                                                          cTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCg                                                                        <  1:696008/96‑1 (MQ=255)
                                                                                          cTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTgg                                                         <  1:967163/111‑1 (MQ=255)
                                                                                           ttGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGAt                            <  2:1265149/139‑1 (MQ=255)
                                                                                            tGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                <  1:657715/70‑1 (MQ=255)
                                                                                            tGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                           >  2:1143680/1‑75 (MQ=255)
                                                                                            tGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATg                           <  1:1286936/139‑1 (MQ=255)
                                                                                            tGGCAGGCCGTAAACAAATCCGCATTCGCGACTGGATTTGCCCTGATATTTCCAGACATCTGTTATCACTTAAGCCATTACAAGCCCGCTTCCGCAGATCTTCCCGTGGCCAGCGATCACCCAGCGCACTATGCAGATg                           >  2:120573/1‑139 (MQ=17)
                                                                                             ggCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTATAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGc                          >  2:671052/1‑139 (MQ=255)
                                                                                             ggCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGc                          >  1:382152/1‑139 (MQ=255)
                                                                                                aGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAt                       <  2:382152/139‑1 (MQ=255)
                                                                                                aGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAt                       <  1:243662/139‑1 (MQ=255)
                                                                                                 ggCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                           <  1:869332/70‑1 (MQ=255)
                                                                                                 ggCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                           >  2:869332/1‑70 (MQ=255)
                                                                                                 ggCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGa                                                                    >  2:278084/1‑93 (MQ=255)
                                                                                                 ggCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAtt                      >  1:924902/1‑139 (MQ=255)
                                                                                                  gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCa                                                                                                                >  1:612805/1‑48 (MQ=255)
                                                                                                  gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                           >  1:18788/1‑69 (MQ=255)
                                                                                                  gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACCAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCa                        >  1:143285/1‑136 (MQ=255)
                                                                                                  gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGc                                                                       <  1:1005032/89‑1 (MQ=255)
                                                                                                  gCCGTAAACAAATCCGCATTCGTAGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGcc                                                                         <  1:470326/87‑1 (MQ=255)
                                                                                                    cGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATc                                                                                                          <  1:129752/52‑1 (MQ=255)
                                                                                                    cGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                <  2:230470/62‑1 (MQ=255)
                                                                                                    cGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                           >  2:702333/1‑67 (MQ=255)
                                                                                                    cGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                              >  2:192216/1‑80 (MQ=255)
                                                                                                    cGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAACCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAg                                          <  2:1153188/116‑1 (MQ=255)
                                                                                                    cGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAACCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAg                                          >  1:1153188/1‑116 (MQ=255)
                                                                                                             aTCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACAt                                                                                                           <  1:712287/42‑1 (MQ=255)
                                                                                                              tCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGcc                                                                         <  1:427173/75‑1 (MQ=255)
                                                                                                              tCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGcc                                                                         >  2:427173/1‑75 (MQ=255)
                                                                                                                 gCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTATCCCATTGCAAGCCCGCTGCCGGAGATATTCCCGTGGCGAGCAATAACCCAGCGCACTATTCGGATGCCATTCGTTATAATGCTCGaa      <  1:120573/139‑1 (MQ=11)
                                                                                                                 gCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGaa      <  2:732871/139‑1 (MQ=255)
                                                                                                                  cATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAAc                                              >  2:824381/1‑98 (MQ=255)
                                                                                                                  cATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGcc                         <  1:439123/119‑1 (MQ=255)
                                                                                                                     tCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGcc  >  2:339845/1‑139 (MQ=21)
                                                                                                                                       |                                                                                                                        
TATTGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCC  >  NC_000913/2068817‑2069072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: