Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	197,574	T→C	S343S (AGT→AGC)	rseP →	inner membrane zinc RIP metalloprotease, RpoE activator, by degrading RseA, cleaved signal peptide endoprotease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	197,574	0	T	C	100.0%	259.0 / NA	71	S343S (AGT→AGC)	rseP	inner membrane zinc RIP metalloprotease, RpoE activator, by degrading RseA, cleaved signal peptide endoprotease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/35);  total (36/35)

GATTGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGTATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTG  >  NC_000913/197439‑197709                                                                                                                                         |                                                                                                                                        gATTGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATg                                                                                                                                      <  1:470140/139‑1 (MQ=255)   ttGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCt                                                                                                                                    >  1:797252/1‑139 (MQ=255)               gTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGTCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGAt                                                                                                                        <  2:797252/139‑1 (MQ=255)                     aGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGACAGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCg                                                                                                                   >  2:1256030/1‑139 (MQ=255)                      gCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGa                                                                                                                                >  1:908446/1‑124 (MQ=255)                      gCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGa                                                                                                                                <  2:908446/124‑1 (MQ=255)                       ccGAAAGTCATTTCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTg                                                                                                                <  2:782948/139‑1 (MQ=255)                       ccGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCAc                                                                                                                     <  1:821179/134‑1 (MQ=255)                       ccGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCAc                                                                                                                     >  2:821179/1‑134 (MQ=255)                          aaaGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGAtg                                                                                                             >  2:1027995/1‑139 (MQ=255)                                aTTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAAc                                                                                                       <  1:247624/139‑1 (MQ=255)                                    cTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaa                                                                                                   <  1:1302643/139‑1 (MQ=255)                                     tttGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGACAGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaa                                                                                                   <  1:1256030/139‑1 (MQ=255)                                       tGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaacaa                                                                                                <  1:855970/139‑1 (MQ=255)                                       tGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaacaa                                                                                                >  2:806845/1‑139 (MQ=255)                                        gCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTCCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaacaac                                                                                               >  2:591260/1‑139 (MQ=255)                                             aTGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAg                                                                                          >  2:1198231/1‑139 (MQ=255)                                                      aaGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGAt                                                                                 <  2:598679/139‑1 (MQ=255)                                                      aaGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGAt                                                                                 <  1:667533/139‑1 (MQ=255)                                                      aaGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGAt                                                                                 <  1:1214127/139‑1 (MQ=255)                                                          tGTACGCCAGTATGGGCCGTTCAACGCCACCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGAGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATctct                                                                             <  1:1177890/139‑1 (MQ=255)                                                            tACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAAc                                                                                                       >  2:698823/1‑111 (MQ=255)                                                            tACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAAc                                                                                                       <  1:698823/111‑1 (MQ=255)                                                            tACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTAt                                                                           >  2:423312/1‑139 (MQ=255)                                                            tACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTAt                                                                           <  1:1022550/139‑1 (MQ=255)                                                            tACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTAt                                                                           >  1:1124194/1‑139 (MQ=255)                                                            tACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTAt                                                                           >  1:525654/1‑139 (MQ=255)                                                            tACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTAATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTAt                                                                           >  1:784411/1‑139 (MQ=255)                                                              cGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCGGATCTCTATCg                                                                         >  1:855535/1‑139 (MQ=255)                                                                         gCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCt                                                              >  1:645096/1‑139 (MQ=255)                                                                           cGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGaa                                                                                                                               >  1:1305333/1‑72 (MQ=255)                                                                           cGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGaa                                                                                                                               <  2:1305333/72‑1 (MQ=255)                                                                           cGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTgg                                                            >  1:1274333/1‑139 (MQ=255)                                                                             ttCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGa                                                                                                                                >  1:687162/1‑69 (MQ=255)                                                                             ttCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGa                                                                                                                                <  2:687162/69‑1 (MQ=255)                                                                             ttCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTg                                                                                                                <  1:1149804/85‑1 (MQ=255)                                                                             ttCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTg                                                                                                                >  2:1149804/1‑85 (MQ=255)                                                                             ttCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGa                                                          >  2:123442/1‑139 (MQ=255)                                                                             ttCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGa                                                          >  1:78729/1‑139 (MQ=255)                                                                             ttCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGa                                                          >  1:115333/1‑139 (MQ=255)                                                                             ttCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGa                                                          >  2:809461/1‑139 (MQ=255)                                                                              tCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGAtg                                                                                                             <  1:580231/87‑1 (MQ=255)                                                                              tCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGAtg                                                                                                             >  2:580231/1‑87 (MQ=255)                                                                              tCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGAt                                                         >  2:404445/1‑139 (MQ=255)                                                                               cAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATg                                                        <  2:681869/139‑1 (MQ=255)                                                                                       tcgtcgAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGc                                                                                                                                     <  1:977375/54‑1 (MQ=255)                                                                                       tcgtcgAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGc                                                                                                                                     >  2:977375/1‑54 (MQ=255)                                                                                       tcgtcgAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGa                                                >  2:569862/1‑139 (MQ=255)                                                                                        cgtcgAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTggg                                                                                      <  2:741447/100‑1 (MQ=255)                                                                                        cgtcgAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTggg                                                                                      >  1:741447/1‑100 (MQ=255)                                                                                                    ggACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAggggg                                                                >  1:599433/1‑110 (MQ=255)                                                                                                    ggACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAggggg                                                                <  2:599433/110‑1 (MQ=255)                                                                                                    ggACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                        >  2:1239413/1‑134 (MQ=255)                                                                                                    ggACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                        <  1:1239413/134‑1 (MQ=255)                                                                                                          aaCGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTAc                             >  1:548370/1‑139 (MQ=255)                                                                                                          aaCGTGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTAc                             >  1:434530/1‑139 (MQ=255)                                                                                                              tGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAg                                                                    <  1:993461/96‑1 (MQ=255)                                                                                                              tGGCAGCTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAg                                                                    >  2:993461/1‑96 (MQ=255)                                                                                                                    cTGA‑TGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTc                   <  2:1274333/139‑1 (MQ=255)                                                                                                                      ga‑tgaAGCTTACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTttat                                >  1:896880/1‑124 (MQ=255)                                                                                                                      ga‑tgaAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACGACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTttat                                <  2:896880/124‑1 (MQ=255)                                                                                                                           aaGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTgcgc             <  1:393666/139‑1 (MQ=255)                                                                                                                              cTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCtta          <  1:1027995/139‑1 (MQ=255)                                                                                                                                 aCGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAgg                                                                   >  2:525179/1‑79 (MQ=255)                                                                                                                                 aCGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAgg                                                                   <  1:525179/79‑1 (MQ=255)                                                                                                                                  cGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGgt                                       >  2:779035/1‑106 (MQ=255)                                                                                                                                  cGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGgt                                       <  1:779035/106‑1 (MQ=255)                                                                                                                                   ggTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGc     <  1:1198231/139‑1 (MQ=255)                                                                                                                                    gTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCg    <  2:78729/139‑1 (MQ=255)                                                                                                                                     tCAGCATGCTGGCAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTTCCTGCCGTTTCTTGCGCTTATTAGCGt   <  2:784411/139‑1 (MQ=255)                                                                                                                                      cAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTg  <  1:569862/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GATTGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGA‑TGAAGCTGACGGTCAGTATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTG  >  NC_000913/197439‑197709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑