Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,090,046 G→A *17* (TAG→TAA hisL → his operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,090,0460GA99.7% 951.7 / NA 290*17* (TAG→TAAhisLhis operon leader peptide
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (135/153);  minor base T (1/0);  total (136/154)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

AATATATAAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGA  >  NC_000913/2089911‑2090180
                                                                                                                                        |                                                                                                                                      
    tataAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCa                                                                                                                                 >  2:567584/1‑139 (MQ=255)
    tataAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCa                                                                                                                                 >  1:1074596/1‑139 (MQ=255)
    tataAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCa                                                                                                                                 >  1:415681/1‑139 (MQ=255)
     ataAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAg                                                                                                                                <  1:977010/139‑1 (MQ=255)
          aaaGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                           <  1:1105279/139‑1 (MQ=255)
          aaaGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                           <  1:567584/139‑1 (MQ=255)
          aaaGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCAACATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                           <  2:850832/139‑1 (MQ=255)
           aaGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtg                                                                                                                          <  1:1047975/139‑1 (MQ=255)
            aGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgt                                                                                                                         >  2:825152/1‑139 (MQ=255)
            aGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgt                                                                                                                         >  1:177588/1‑139 (MQ=255)
              cccTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCg                                                                                                                             <  1:673735/133‑1 (MQ=255)
              cccTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtgt                                                                                                                       >  2:452898/1‑139 (MQ=255)
              cccTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtgt                                                                                                                       >  1:1120186/1‑139 (MQ=255)
              cccTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGCGAtgtgtt                                                                                                                      >  1:546745/1‑138 (MQ=255)
                    cTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGa                                                                                                                 <  2:396498/139‑1 (MQ=255)
                     tttCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                <  1:825152/139‑1 (MQ=255)
                     tttCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                <  1:917068/139‑1 (MQ=255)
                              tGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtg                                                                                                                        <  2:363400/122‑1 (MQ=255)
                               gAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGa                                                                                                      <  1:506288/139‑1 (MQ=255)
                                 aaGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCCTCACCATCATCCTGACTAATCTTTCAGGCGAtgtg                                                                                                                        >  2:454150/1‑119 (MQ=255)
                                 aaGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtg                                                                                                                        <  1:454150/119‑1 (MQ=255)
                                      ttttAGTTAAAAAGACACCATTTAATAAACCATCACCAGGAATTTTTAGGCCCCGCGTCCAATTAAACCCCCACCACCATCACCACCATCCTGCCTAACTTTTCGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                              <  2:546745/138‑1 (MQ=255)
                                       tttAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                              <  2:669353/139‑1 (MQ=255)
                                           ggTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAgtggt                                                                                          <  1:219622/139‑1 (MQ=255)
                                            gTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAgtggtg                                                                                         <  1:988052/139‑1 (MQ=255)
                                                aaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                <  2:584004/112‑1 (MQ=255)
                                                aaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCa                                                                                                        <  2:638186/120‑1 (MQ=255)
                                                aaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCa                                                                                                        >  1:638186/1‑120 (MQ=255)
                                                aaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATg                                                                                     >  1:1079437/1‑139 (MQ=255)
                                                 aaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGa                                                                                    >  1:723705/1‑139 (MQ=255)
                                                   gACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAAc                                                                                  <  1:254861/139‑1 (MQ=255)
                                                     cATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACAt                                                                                                           <  1:735888/112‑1 (MQ=255)
                                                     cATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACAt                                                                                                           >  2:735888/1‑112 (MQ=255)
                                                      aTCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCg                                                                                                                             <  1:369129/93‑1 (MQ=255)
                                                      aTCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCg                                                                                                                             >  2:369129/1‑93 (MQ=255)
                                                      aTCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                           >  1:1007002/1‑95 (MQ=255)
                                                       tCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCAt                                                                              >  2:798118/1‑139 (MQ=255)
                                                               aTAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGc                                                                      <  2:1120186/139‑1 (MQ=255)
                                                                tAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                           >  2:109220/1‑85 (MQ=255)
                                                                  aaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGcccc                                                                   <  1:798118/139‑1 (MQ=255)
                                                                   aCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGccccc                                                                  <  1:1301255/139‑1 (MQ=255)
                                                                   aCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATAATCCTGACTAATCTTTCAgg                                                                                                                               <  2:771836/78‑1 (MQ=255)
                                                                     aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAgg                                                                                                                               <  2:892942/76‑1 (MQ=255)
                                                                     aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAg                                                                                                                                <  1:243711/75‑1 (MQ=255)
                                                                     aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtg                                                                                                                          >  2:1068462/1‑81 (MQ=255)
                                                                        cacaGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAgg                                                                                                                               >  2:262157/1‑73 (MQ=255)
                                                                         acaGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGa                                                                                                                 <  1:331674/86‑1 (MQ=255)
                                                                         acaGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGa                                                                                                                 >  2:331674/1‑86 (MQ=255)
                                                                         acaGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGa                                                            <  2:73926/139‑1 (MQ=255)
                                                                             gagaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTc                                                                                                 <  2:161913/99‑1 (MQ=255)
                                                                             gagaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCa                                                         <  2:979942/139‑1 (MQ=255)
                                                                              agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAg                                                                                                               >  2:72691/1‑84 (MQ=255)
                                                                              agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCAc                                                        <  2:162470/139‑1 (MQ=255)
                                                                              agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCAc                                                        >  2:228247/1‑139 (MQ=255)
                                                                                      aTGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                <  2:373473/139‑1 (MQ=255)
                                                                                          cacGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGCCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATg                                                                                     >  2:1165822/1‑98 (MQ=255)
                                                                                          cacGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGaaa                                                                        <  2:599233/111‑1 (MQ=255)
                                                                                          cacGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGaaa                                                                        >  1:726983/1‑111 (MQ=255)
                                                                                            cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtg                                                                                                                          <  2:559258/59‑1 (MQ=255)
                                                                                            cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                <  1:162463/69‑1 (MQ=255)
                                                                                            cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                >  2:245237/1‑69 (MQ=255)
                                                                                            cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttt                                          >  1:1243726/1‑139 (MQ=255)
                                                                                                ttCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCttt                                                                                                                                   <  2:1027912/46‑1 (MQ=255)
                                                                                                     tttAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg                                 <  2:293565/139‑1 (MQ=255)
                                                                                                     tttAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg                                 <  2:1140696/139‑1 (MQ=255)
                                                                                                         aaCACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtg                                                                                                                        <  1:77330/48‑1 (MQ=255)
                                                                                                             ccaccaTCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                           <  2:191934/41‑1 (MQ=255)
                                                                                                             ccaccaTCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                <  1:270463/52‑1 (MQ=255)
                                                                                                             ccaccaTCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAg                                                                                                               >  1:1156657/1‑53 (MQ=255)
                                                                                                             ccaccaTCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGaa                                                                                   >  2:100604/1‑81 (MQ=255)
                                                                                                              caccatcatcaccatcatCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATgaga                                                                          >  2:212566/1‑89 (MQ=255)
                                                                                                              caccatcatcaccatcatCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                                 >  2:429150/1‑114 (MQ=255)
                                                                                                              caccatcatcaccatcatCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                                 <  1:429150/114‑1 (MQ=255)
                                                                                                              caccatcatcaccatcatCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATa                        <  2:1246807/139‑1 (MQ=255)
                                                                                                               accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                >  1:573487/1‑50 (MQ=255)
                                                                                                               accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGa                                                                                                      <  1:766908/60‑1 (MQ=255)
                                                                                                               accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg                                 <  2:1196774/129‑1 (MQ=255)
                                                                                                               accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg                                 >  1:1196774/1‑129 (MQ=255)
                                                                                                               accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATaa                       <  2:513188/139‑1 (MQ=255)
                                                                                                                      tcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATa                        <  1:1005620/131‑1 (MQ=255)
                                                                                                                      tcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCa                >  2:350667/1‑139 (MQ=255)
                                                                                                                        accatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGa              <  1:1179676/139‑1 (MQ=255)
                                                                                                                           atcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCg                                                  >  2:1079298/1‑100 (MQ=255)
                                                                                                                            tcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                              >  2:685860/1‑55 (MQ=255)
                                                                                                                             catcCTGACTAATCTTTCAGGCGATGTGTGCTGGa                                                                                                                 <  1:926610/35‑1 (MQ=255)
                                                                                                                             catcCTGACTAATCTTTCAGGCGATGTGTGCTGGa                                                                                                                 >  2:926610/1‑35 (MQ=255)
                                                                                                                             catcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGt         >  1:1092372/1‑139 (MQ=255)
                                                                                                                               tcCTGACTATTCTTTCAGGCGATGTGTGCTGTAAGACATCCAGATCGACCAGTGGTCCATGAACGCATTGGAAAGCCCCGGTAAGCGCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAGCGGTTCAGACAGGctt       >  2:501633/1‑139 (MQ=255)
                                                                                                                               tcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAg                                                                                                               <  1:87748/35‑1 (MQ=255)
                                                                                                                               tcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                              <  1:357578/52‑1 (MQ=255)
                                                                                                                               tcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGttt       >  2:751215/1‑139 (MQ=255)
                                                                                                                               tcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGttt       >  2:1183109/1‑139 (MQ=255)
                                                                                                                                ccTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                              >  1:59035/1‑51 (MQ=255)
                                                                                                                                    aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTc                                                                                                         >  1:192272/1‑36 (MQ=255)
                                                                                                                                    aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCCGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                <  2:869490/93‑1 (MQ=255)
                                                                                                                                    aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                              <  1:1140487/95‑1 (MQ=255)
                                                                                                                                    aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg                                 <  1:651372/108‑1 (MQ=255)
                                                                                                                                    aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg                                 >  2:651372/1‑108 (MQ=255)
                                                                                                                                    aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAaga  >  1:1189814/1‑139 (MQ=255)
                                                                                                                                    aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCGTCCggg                                                >  1:869490/1‑93 (MQ=255)
                                                                                                                                        |                                                                                                                                      
AATATATAAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGA  >  NC_000913/2089911‑2090180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: