Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,226,509	C→T	*254* (TAG→TAA)	yohF ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,226,509	0	C	T	100.0%	101.8 / NA	33	*254* (TAG→TAA)	yohF	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/19);  total (14/19)

CCATTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCAAAACGC  >  NC_000913/2226382‑2226644                                                                                                                                |                                                                                                                                        ccATTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGtt                                                                                                                              <  1:127699/139‑1 (MQ=255)           ggATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGa                                                                                                                    <  1:472175/139‑1 (MQ=255)                 ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTAtt                                                                                                                                     <  2:6164/116‑1 (MQ=255)                 ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTAtt                                                                                                                                     >  1:6164/1‑116 (MQ=255)                 ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCAACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGcc                                                                                                              >  2:840855/1‑139 (MQ=255)                                    aCATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCa                                                                                           <  1:619717/139‑1 (MQ=255)                                        ccGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAgccgc                                                                                                 >  2:556925/1‑129 (MQ=255)                                        ccGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAgccgc                                                                                                 <  1:556925/129‑1 (MQ=255)                                              tACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGa                                                                                 <  2:1311004/139‑1 (MQ=255)                                                  aTGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGc                                                                             >  1:998849/1‑139 (MQ=255)                                                        gATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCAt                                                                                              >  2:585491/1‑116 (MQ=255)                                                        gATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCAt                                                                                              <  1:585491/116‑1 (MQ=255)                                                        gATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCggtg                                                                       >  1:14900/1‑139 (MQ=255)                                                              acgCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTaa                                                                 >  1:774529/1‑139 (MQ=255)                                                               cgCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCa                                                                                           <  1:798463/112‑1 (MQ=255)                                                               cgCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCa                                                                                           >  2:798463/1‑112 (MQ=255)                                                               cgCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAAc                                                                                   >  2:347158/1‑120 (MQ=255)                                                               cgCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAAc                                                                                   <  1:347158/120‑1 (MQ=255)                                                                   tgtgCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTgc                                                            <  2:14900/139‑1 (MQ=255)                                                                   tgtgCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTgc                                                            <  1:1270175/139‑1 (MQ=255)                                                                             ttCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGaa                                                  <  1:522933/139‑1 (MQ=255)                                                                                    cGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCa                                                                                               <  2:111637/87‑1 (MQ=255)                                                                                    cGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCa                                                                                               >  1:111637/1‑87 (MQ=255)                                                                                                tACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATCTGCGCCCTCCGAACAAAGCCACACCACCAGGc                               <  1:241047/139‑1 (MQ=255)                                                                                                 aCCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACaa                                               <  1:624663/122‑1 (MQ=255)                                                                                                 aCCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACaa                                               >  2:624663/1‑122 (MQ=255)                                                                                                       tGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTgc                                                            >  1:727824/1‑103 (MQ=255)                                                                                                       tGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTgc                                                            <  2:727824/103‑1 (MQ=255)                                                                                                       tGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAAt                        >  1:611080/1‑139 (MQ=255)                                                                                                               ccACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCg                <  1:366077/139‑1 (MQ=255)                                                                                                                   gCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCgg                                                                         <  2:19378/78‑1 (MQ=255)                                                                                                                   gCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCgg                                                                         >  1:19378/1‑78 (MQ=255)                                                                                                                          aCTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCaaaa     <  2:611080/139‑1 (MQ=255)                                                                                                                             gcgtTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCAAAAcgc  <  1:29723/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                        CCATTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCAAAACGC  >  NC_000913/2226382‑2226644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑