Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,334,336	C→T	pseudogene (67/4605 nt) *208* (TAG→TAA)	yfaS ← yfaT ←	pseudogene, bacterial alpha2‑macroglobulin YfaS variant family, putative membrane protein DUF1175 family protein, putative host defense protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,334,336	0	C	T	100.0%	63.2 / NA	21	pseudogene (67/4605 nt) *208* (TAG→TAA)	yfaS yfaT	pseudogene, bacterial alpha2‑macroglobulin YfaS variant family, putative membrane protein DUF1175 family protein, putative host defense protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/11);  total (10/11)

TGCTAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGCTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAGACTGACTGCGCGCATTCCGTTGTCAGT  >  NC_000913/2334210‑2334461                                                                                                                               |                                                                                                                              tgctAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGa                                                                                                                   <  2:650967/139‑1 (MQ=255)        aCTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTa                                                                                                            >  2:1135285/1‑139 (MQ=255)        aCTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTa                                                                                                            <  2:1019435/139‑1 (MQ=255)             tgTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGa                                                                                                       >  2:937875/1‑139 (MQ=255)                      gcaaAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCa                                                                                              <  1:322514/139‑1 (MQ=255)                        aaaaaGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAAt                                                                                            >  2:75641/1‑139 (MQ=255)                              aaTGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAAtt                                                                                      <  2:69181/139‑1 (MQ=255)                                tGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGa                                                                                                                   <  1:248578/108‑1 (MQ=255)                                tGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGa                                                                                                                   >  2:248578/1‑108 (MQ=255)                                 gTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTggg                                                                                   <  2:1044218/139‑1 (MQ=255)                                        ctgCAGGCTGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTATTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTg                                                                               <  1:648154/134‑1 (MQ=255)                                        cGGCAGGCTGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTATTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTg                                                                               >  2:648154/1‑136 (MQ=255)                                          gCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATc                                                                          <  1:97318/139‑1 (MQ=255)                                              gcggcgCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTg                                                                      >  1:386978/1‑139 (MQ=255)                                              gcggcgCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTg                                                                      >  2:918830/1‑139 (MQ=255)                                                                    aagCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTcc                                                <  1:918830/139‑1 (MQ=255)                                                                                      aGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAgttgt                                  >  1:726769/1‑135 (MQ=255)                                                                                      aGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAgttgt                                  <  2:726769/135‑1 (MQ=255)                                                                                                 ttCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAGACTGACTgcg                   >  1:193098/1‑139 (MQ=255)                                                                                                       caAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAGACTGACTGCGCGCAtt             <  1:1135285/139‑1 (MQ=255)                                                                                                                  gACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAGACTGACTGCGCGCATTCCGTTGTCAGt  >  2:447733/1‑139 (MQ=255)                                                                                                                               |                                                                                                                              TGCTAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGCTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAGACTGACTGCGCGCATTCCGTTGTCAGT  >  NC_000913/2334210‑2334461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑