Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,438,548 T→C V200A (GTG→GCG)  flk → putative flagella assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,438,5480TC100.0% 178.8 / NA 50V200A (GTG→GCG) flkputative flagella assembly protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/27);  total (23/27)

CAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTA  >  NC_000913/2438412‑2438678
                                                                                                                                        |                                                                                                                                  
cagcagCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGa                                                                                                                                  >  1:250594/1‑139 (MQ=255)
      cgcgAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGATGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCGGGAACTTTCCGGGGCGAAAGATg                                                                                                                            >  2:866447/1‑139 (MQ=255)
       gcgAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAATCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATgg                                                                                                                           >  1:870000/1‑139 (MQ=255)
        cgAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGc                                                                                                                          <  1:213794/139‑1 (MQ=255)
                ccgaccgTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAAt                                                                                                                  <  1:530292/139‑1 (MQ=255)
                     cgTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAg                                                                                                             >  2:957263/1‑139 (MQ=255)
                      gTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGc                                                                                                            >  2:990636/1‑139 (MQ=255)
                        ccTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGa                                                                                                          >  1:710497/1‑139 (MQ=255)
                            tattaCCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACt                                                                                                      <  1:957263/139‑1 (MQ=255)
                               taCCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGtt                                                                                                   <  2:580129/139‑1 (MQ=255)
                                  cggcggAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGTAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTaa                                                                                                >  2:907317/1‑139 (MQ=255)
                                  cggcggAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTaa                                                                                                >  1:46071/1‑139 (MQ=255)
                                   ggcggAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAAc                                                                                               >  2:1283150/1‑139 (MQ=255)
                                    gcggAGCACAATGCGCTAAAACAGCTGGTGACCAAATTTGCGGCGGCGACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAAcc                                                                                              >  1:836359/1‑139 (MQ=255)
                                        aGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCt                                                                                          >  2:1051701/1‑139 (MQ=255)
                                          cacaATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTgg                                                                                        >  1:497520/1‑139 (MQ=255)
                                              aTGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGAc                                                                                    <  2:1256133/139‑1 (MQ=255)
                                              aTGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGAc                                                                                    <  2:1312268/139‑1 (MQ=255)
                                              aTGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGAc                                                                                    <  1:907317/139‑1 (MQ=255)
                                               tGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGt                                                                                       >  1:1191758/1‑135 (MQ=255)
                                               tGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGt                                                                                       <  2:1191758/135‑1 (MQ=255)
                                                gcgcTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCt                                                                                  <  2:870000/139‑1 (MQ=255)
                                                           gCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGgcg                                                                       >  1:1071992/1‑139 (MQ=255)
                                                                tGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCa                                                                  <  2:46071/139‑1 (MQ=255)
                                                                  aCCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAg                                                                                                             <  1:786815/94‑1 (MQ=255)
                                                                  aCCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAg                                                                                                             >  2:786815/1‑94 (MQ=255)
                                                                          tGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGc                                                        >  2:947540/1‑139 (MQ=255)
                                                                          tGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGc                                                        <  2:1071992/139‑1 (MQ=255)
                                                                            cggcggCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATgg                                                                                                                           >  1:134423/1‑70 (MQ=255)
                                                                            cggcggCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATgg                                                                                                                           <  2:134423/70‑1 (MQ=255)
                                                                                            aCCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACg                                      <  1:947540/139‑1 (MQ=255)
                                                                                              ccAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCt                                    <  1:435737/139‑1 (MQ=255)
                                                                                                 gCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCTGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGa                                 <  1:866447/139‑1 (MQ=255)
                                                                                                 gCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGa                                 <  2:1193653/139‑1 (MQ=255)
                                                                                                 gCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGa                                 <  2:250594/139‑1 (MQ=255)
                                                                                                  cAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTa                                                                                                 <  1:756477/74‑1 (MQ=255)
                                                                                                  cAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTa                                                                                                 >  2:756477/1‑74 (MQ=255)
                                                                                                   aaaCAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAAt                               <  1:1178148/139‑1 (MQ=255)
                                                                                                        gATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGAc                                                                                    >  1:919713/1‑81 (MQ=255)
                                                                                                        gATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGAc                                                                                    <  2:919713/81‑1 (MQ=255)
                                                                                                        gATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCt                                    <  2:251879/129‑1 (MQ=255)
                                                                                                        gATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCt                                    >  1:251879/1‑129 (MQ=255)
                                                                                                        gATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTa                          <  1:990636/139‑1 (MQ=255)
                                                                                                              gCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCt                                    >  2:61636/1‑123 (MQ=255)
                                                                                                              gCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCt                                    <  1:61636/123‑1 (MQ=255)
                                                                                                                       gCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTaaaa           <  1:445815/139‑1 (MQ=255)
                                                                                                                           gAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAaacaac       <  2:710497/139‑1 (MQ=255)
                                                                                                                                ttCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGt                                                                                       <  1:917785/54‑1 (MQ=255)
                                                                                                                                ttCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGt                                                                                       >  2:917785/1‑54 (MQ=255)
                                                                                                                                ttCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTa  >  1:983516/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                  
CAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTA  >  NC_000913/2438412‑2438678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑