Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	242,255	T→C	D350G (GAT→GGT)	fadE ←	acyl coenzyme A dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	242,255	0	T	C	100.0%	101.3 / NA	29	D350G (GAT→GGT)	fadE	acyl coenzyme A dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/16);  total (13/16)

AGCGCTACCGATTTCACGCCGCCGGTTGAGTTGGAAGGCAGGGTGATGCCGCGGCCTACCGAGAGGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATG  >  NC_000913/242132‑242377                                                                                                                            |                                                                                                                           aGCGCTACCGATTTCACGCCGCCGGTTGAGTTGGAAGGCAGGGTGATGCCGCGGCCTACCGAGAGGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGTCCGCCGATGATGTAACCGATCGGCACGAAGa                                                                                                             <  2:1015224/139‑1 (MQ=255)   cgcTACCGATTTCACGCCGCCGGTTGAGTTGGAAGGCAGGGTGATGCCGCGGCCTACCGAGAGGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACa                                                                                                           >  2:1196775/1‑139 (MQ=255)            tttCACGCCGCCGGTTGAGTTGGAAGGCAGGGTGATGCCGCGGCCTACCGAGAGGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACcg                                                                                                  <  1:1196775/139‑1 (MQ=255)                 cgccgcCGGTTGAGTTGGAAGGCAGGGTGATGCCGCGGCCTACCGAGAGGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGt                                                                                             <  2:471124/139‑1 (MQ=255)                    cgccgGTTGAGTTGGAAGGCAGGGTGATGCCGCGGCCTACGGAGAGGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCgg                                                                                          >  2:977769/1‑139 (MQ=255)                           tGAGTTGGAAGGCAGGGTGATGCCGCGGCCTACCGAGAGGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTc                                                                                   <  1:38003/139‑1 (MQ=255)                                       cAGGGTGATGCCGCGGCCTACCGAGAGGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGAAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACg                                                                       >  2:1236850/1‑139 (MQ=255)                                                gCCGCGGCCTACCGAGAGGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCggg                                                              >  1:983876/1‑139 (MQ=255)                                                  cgcgGCCTACCGAGAGGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGaa                                                            <  2:983876/139‑1 (MQ=255)                                                           ccGAGAGGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGcgacg                                                   <  1:977769/139‑1 (MQ=255)                                                            cGAGAGGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGcgacga                                                  <  2:599344/139‑1 (MQ=255)                                                               gagGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCa                                               <  2:693533/139‑1 (MQ=255)                                                                     cTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGa                                                                                                             >  2:235613/1‑71 (MQ=255)                                                                     cTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGa                                                                                                             <  1:235613/71‑1 (MQ=255)                                                                                tCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCgt                              >  2:519741/1‑139 (MQ=255)                                                                                 ccGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCgtg                             <  2:956732/139‑1 (MQ=255)                                                                                  cGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGaa                                                                                                               >  1:681218/1‑56 (MQ=255)                                                                                  cGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGaa                                                                                                               <  2:681218/56‑1 (MQ=255)                                                                                  cGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCgtgg                            >  2:514415/1‑139 (MQ=255)                                                                                     cAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGtggtgg                         >  1:259623/1‑139 (MQ=255)                                                                                         cTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTgg                     <  2:1194363/139‑1 (MQ=255)                                                                                              ccGGCCATTTTCGGCCCGCCGATGATGAAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCa                <  1:1236850/139‑1 (MQ=255)                                                                                                  ccATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCgtgg                            <  2:605107/123‑1 (MQ=255)                                                                                                  ccATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCgtgg                            >  1:605107/1‑123 (MQ=255)                                                                                                  ccATTTTCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAgcgc            >  2:438099/1‑139 (MQ=255)                                                                                                      tttCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTAc                                                                        <  2:1275622/75‑1 (MQ=255)                                                                                                      tttCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTAc                                                                        >  1:1275622/1‑75 (MQ=255)                                                                                                       ttCGGCCCGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAgg       >  2:462618/1‑139 (MQ=255)                                                                                                            cccGCCGATGATGTAACCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATg  <  1:438099/139‑1 (MQ=255)                                                                                                                            |                                                                                                                           AGCGCTACCGATTTCACGCCGCCGGTTGAGTTGGAAGGCAGGGTGATGCCGCGGCCTACCGAGAGGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATG  >  NC_000913/242132‑242377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑