Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,478,092	A→G	V82V (GTA→GTG)	dsdX →	D‑serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,478,092	0	A	G	100.0%	150.0 / NA	42	V82V (GTA→GTG)	dsdX	D‑serine transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/22);  total (20/22)

GAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTG  >  NC_000913/2477968‑2478215                                                                                                                             |                                                                                                                            gAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCaga                                                                                                               <  1:1194069/139‑1 (MQ=255)         atgGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTg                                                                                                       <  1:837787/139‑1 (MQ=255)            ggCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTc                                                                                                    >  1:368758/1‑139 (MQ=255)                 ggggCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGaca                                                                                               >  1:134218/1‑139 (MQ=255)                   ggCCACTGGATATGGTAAATTCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACt                                                                                             >  2:771893/1‑139 (MQ=255)                       aCTGGATATGGTAAATGCTATTGAAAGTGGTATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTACGGGGCCGCAGAAAGAATTGGTCTGACACTTCaa                                                                                         >  2:246539/1‑139 (MQ=255)                         tGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGTGCCGCAGAAAGAATTGGTCTGACACTTCAACg                                                                                       <  2:542332/139‑1 (MQ=255)                               tGGTCAATGTTATTTACAGTTGAATTGGCGGAATGCAGGGGTCCCTCGCAGCGCTTATCGGCCTTGGCACGATACCGGGAAAAATGATGCAAGTGTTCGGGGCCCCAGAAAGAATCCGTCTGACAATTCAACGCTGCCg                                                                                 <  2:1101339/139‑1 (MQ=255)                                     aTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCt                                                                           >  1:575727/1‑139 (MQ=255)                                              aaaGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGa                                                                  <  1:1041877/139‑1 (MQ=255)                                                 gTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGt                                                               >  1:520713/1‑139 (MQ=255)                                                    gAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGt                                                                     >  2:286821/1‑130 (MQ=255)                                                    gAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGt                                                                     <  1:286821/130‑1 (MQ=255)                                                     aaTTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGa                                                                  <  2:935263/132‑1 (MQ=255)                                                     aaTTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGa                                                                  >  1:935263/1‑132 (MQ=255)                                                     aaTTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCAtt                                                           >  2:645714/1‑139 (MQ=255)                                                          gCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGt                                                      <  1:246539/139‑1 (MQ=255)                                                                              gCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTtgtg                                  <  2:520713/139‑1 (MQ=255)                                                                                    gTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCg                                                                                                                   <  2:81978/52‑1 (MQ=255)                                                                                    gTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCg                                                                                                                   >  1:81978/1‑52 (MQ=255)                                                                                     ttATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCAc                           >  1:1077646/1‑139 (MQ=255)                                                                                      tATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGAGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                          <  1:547288/139‑1 (MQ=255)                                                                                        tCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCt                        <  1:645714/139‑1 (MQ=255)                                                                                        tCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCt                        <  2:368758/139‑1 (MQ=255)                                                                                            ccTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCtgttt                    >  1:278606/1‑139 (MQ=255)                                                                                               tGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGc                                                                                <  1:415722/76‑1 (MQ=255)                                                                                               tGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGc                                                                                >  2:415722/1‑76 (MQ=255)                                                                                               tGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                          <  1:481537/130‑1 (MQ=255)                                                                                               tGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                          >  2:481537/1‑130 (MQ=255)                                                                                               tGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCtgtttgtt                 >  1:212675/1‑139 (MQ=255)                                                                                                    cGATACTGGGAAAAATGATGGTAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGt            <  1:771893/139‑1 (MQ=255)                                                                                                      aTACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGtt                                               <  2:656708/102‑1 (MQ=255)                                                                                                      aTACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGtt                                               >  1:656708/1‑102 (MQ=255)                                                                                                       tACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCt                                                                                                   <  2:360847/49‑1 (MQ=255)                                                                                                       tACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCt                                                                                                   >  1:360847/1‑49 (MQ=255)                                                                                                          tGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGt                                                                     <  2:1262789/76‑1 (MQ=255)                                                                                                          tGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGt                                                                     >  1:1262789/1‑76 (MQ=255)                                                                                                              aaaaaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTg  <  2:134218/139‑1 (MQ=255)                                                                                                                 aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGa                                                                                                 <  1:885282/41‑1 (MQ=255)                                                                                                                 aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGa                                                                                                 >  2:885282/1‑41 (MQ=255)                                                                                                                      tgGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGcgtc     >  2:259127/1‑128 (MQ=255)                                                                                                                      tgGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGcgtc     <  1:259127/128‑1 (MQ=255)                                                                                                                             |                                                                                                                            GAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTG  >  NC_000913/2477968‑2478215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑