Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,876,944	G→A	A122T (GCC→ACC)	iap →	aminopeptidase in alkaline phosphatase isozyme conversion

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,876,944	0	G	A	100.0%	143.2 / NA	45	A122T (GCC→ACC)	iap	aminopeptidase in alkaline phosphatase isozyme conversion
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/20);  total (25/20)

GTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTA  >  NC_000913/2876816‑2877067                                                                                                                                 |                                                                                                                            gTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAg                                                                                                                   >  2:28950/1‑139 (MQ=255) gTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAg                                                                                                                   >  1:949171/1‑139 (MQ=255)   cGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCg                                                                                                                 >  2:187219/1‑139 (MQ=255)   cGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCg                                                                                                                 >  1:977338/1‑139 (MQ=255)     gTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGAt                                                                                                               >  1:1216589/1‑139 (MQ=255)              taCCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTAACACCCCGCTGAGCGATGCTGACGcc                                                                                                      >  2:835381/1‑139 (MQ=255)                  gcccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATg                                                                                                  <  2:977338/139‑1 (MQ=255)                  gcccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATg                                                                                                  >  1:1066862/1‑139 (MQ=255)                  gcccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATg                                                                                                  <  1:28950/139‑1 (MQ=255)                   cccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAGAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGc                                                                                                 >  1:178265/1‑139 (MQ=255)                   cccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGc                                                                                                 >  1:770405/1‑139 (MQ=255)                    ccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCg                                                                                                                        <  1:1046648/115‑1 (MQ=255)                    ccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCg                                                                                                                        >  2:1046648/1‑115 (MQ=255)                    ccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGcc                                                                                                >  1:478477/1‑139 (MQ=255)                                   gagCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg                                                                                 <  2:739796/139‑1 (MQ=255)                                       tGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGt                                                                             >  1:885728/1‑139 (MQ=255)                                           aTAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACa                                                                         <  2:885728/139‑1 (MQ=255)                                              aCGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAgg                                                                      >  2:870597/1‑139 (MQ=255)                                                        aaGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgatgat                                                            >  2:7819/1‑139 (MQ=255)                                                        aaGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgatgat                                                            <  1:308362/139‑1 (MQ=255)                                                                tGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccgc                                                    >  1:403964/1‑139 (MQ=255)                                                                  aTTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAg                                                  >  1:663318/1‑139 (MQ=255)                                                                   ttGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAgg                                                 >  2:442333/1‑139 (MQ=255)                                                                                aGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAAtctc                                                                                          >  1:1020981/1‑85 (MQ=255)                                                                                aGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAAtctc                                                                                          <  2:1020981/85‑1 (MQ=255)                                                                                      aGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGaa                              <  1:863558/139‑1 (MQ=255)                                                                                            gcagcaGATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCa                        >  1:490945/1‑139 (MQ=255)                                                                                             cagcagATCATCATTATGGCGCATCTGGATACTTACACCCCg                                                                                                                        >  2:801119/1‑42 (MQ=255)                                                                                             cagcagATCATCATTATGGCGCATCTGGATACTTACACCCCg                                                                                                                        <  1:801119/42‑1 (MQ=255)                                                                                               gcagATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGATTGGATGATAATGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGaa                     <  1:835381/139‑1 (MQ=255)                                                                                                cagATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAAc                    <  2:490945/139‑1 (MQ=255)                                                                                                  gATCATCATTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGc                  <  1:680787/139‑1 (MQ=255)                                                                                                     catcatTATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTg               <  2:949171/139‑1 (MQ=255)                                                                                                          ttATGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGaaaaa          <  2:478477/139‑1 (MQ=255)                                                                                                             tGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgcc                                                      <  1:721599/92‑1 (MQ=255)                                                                                                             tGGCGCATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgcc                                                      >  2:721599/1‑92 (MQ=255)                                                                                                               gcgcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGc     <  1:7819/139‑1 (MQ=255)                                                                                                                cgcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGc                                                                                                       <  1:409790/40‑1 (MQ=255)                                                                                                                cgcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGc                                                                                                       >  2:409790/1‑40 (MQ=255)                                                                                                                cgcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATg                                    <  1:108594/107‑1 (MQ=255)                                                                                                                cgcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATg                                    >  2:108594/1‑107 (MQ=255)                                                                                                                cgcATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGcc    >  1:1155698/1‑139 (MQ=255)                                                                                                                  cATCTGGATACTTACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTa  <  2:403964/139‑1 (MQ=255)                                                                                                                             ttACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATa        <  1:1130090/122‑1 (MQ=255)                                                                                                                             ttACACCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATa        >  2:1130090/1‑122 (MQ=255)                                                                                                                                 |                                                                                                                            GTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTA  >  NC_000913/2876816‑2877067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑