Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,905,532 T→C intergenic (‑114/‑25) queE ← / → yqcG 7‑carboxy‑7‑deazaguanine synthase, queosine biosynthesis/membrane stress resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,905,5320TC100.0% 86.9 / NA 25intergenic (‑114/‑25)queE/yqcG7‑carboxy‑7‑deazaguanine synthase, queosine biosynthesis/membrane stress resistance protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/14);  total (11/14)

ATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTA  >  NC_000913/2905396‑2905634
                                                                                                                                        |                                                                                                      
aTCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaa                                                                                                      <  1:968926/139‑1 (MQ=255)
        aaTCGGGTACTGCATAGCATTCTCTGTGAAGTGGATGATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTaaa                                                                                              <  1:826605/139‑1 (MQ=255)
           cGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAggg                                                                                           <  1:1195456/139‑1 (MQ=255)
            gggTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGa                                                                                          <  1:977206/139‑1 (MQ=255)
                    cATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGta                                                                                  >  2:1260517/1‑139 (MQ=255)
                            tctcTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCa                                                                          >  2:492401/1‑139 (MQ=255)
                                       tGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATaa                                                               >  1:728505/1‑139 (MQ=255)
                                             aTTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGaaaa                                                         <  1:671667/139‑1 (MQ=255)
                                                   aaTTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaa                                                                                                      >  2:642078/1‑88 (MQ=255)
                                                   aaTTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaa                                                                                                      <  1:642078/88‑1 (MQ=255)
                                                         tGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGt                                                                                        >  1:225021/1‑96 (MQ=255)
                                                         tGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGt                                                                                        <  2:225021/96‑1 (MQ=255)
                                                                ccTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTaa                                                                                               <  1:240974/82‑1 (MQ=255)
                                                                ccTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTaa                                                                                               >  2:240974/1‑82 (MQ=255)
                                                                ccTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGa                                                                                          <  1:207298/87‑1 (MQ=255)
                                                                ccTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGa                                                                                          >  2:207298/1‑87 (MQ=255)
                                                                   gCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACa                                   <  1:1260517/139‑1 (MQ=255)
                                                                        aacaaTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTATTCATGTCAAAACATTCAc                              <  2:728505/139‑1 (MQ=255)
                                                                                  tCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCagaag                                                                      >  1:234055/1‑89 (MQ=255)
                                                                                  tCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCagaag                                                                      <  2:234055/89‑1 (MQ=255)
                                                                                                 gTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaa                                                                                                      >  1:232586/1‑42 (MQ=255)
                                                                                                 gTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaa                                                                                                      <  2:232586/42‑1 (MQ=255)
                                                                                                        gcaggTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGaaaa                                                         <  2:227851/80‑1 (MQ=255)
                                                                                                        gcaggTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGaaaa                                                         >  1:227851/1‑80 (MQ=255)
                                                                                                        gcaggTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAAt                                               <  2:643992/90‑1 (MQ=255)
                                                                                                        gcaggTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAAt                                               >  1:643992/1‑90 (MQ=255)
                                                                                                            gTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAgg                                                                                            <  1:657889/41‑1 (MQ=255)
                                                                                                            gTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAgg                                                                                            >  2:657889/1‑41 (MQ=255)
                                                                                                                                   atCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTa  <  2:1055532/108‑1 (MQ=255)
                                                                                                                                   atCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTa  >  1:1055532/1‑108 (MQ=255)
                                                                                                                                        |                                                                                                      
ATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTA  >  NC_000913/2905396‑2905634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: