Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,958,309	T→C	A192A (GCA→GCG)	ptrA ←	protease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,958,309	0	T	C	100.0%	123.8 / NA	35	A192A (GCA→GCG)	ptrA	protease III
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/16);  total (19/16)

TAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTC  >  NC_000913/2958178‑2958434                                                                                                                                    |                                                                                                                              tAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGt                                                                                                                        >  1:583418/1‑139 (MQ=255)        ccGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGt                                                                                                                 >  1:531366/1‑139 (MQ=255)           ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTgcgc                                                                                                                             >  1:1059344/1‑124 (MQ=255)           ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTc                                                                                                              >  2:1133005/1‑139 (MQ=255)           ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTc                                                                                                              >  1:422781/1‑139 (MQ=255)           ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTc                                                                                                              >  1:586243/1‑139 (MQ=255)           ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGAGTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTc                                                                                                              >  1:780384/1‑139 (MQ=255)                      ttGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCt                                                                                                   >  2:443990/1‑139 (MQ=255)                                       ctcGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCg                                                                                  >  1:256664/1‑139 (MQ=255)                                         cGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCAt                                                                                      >  1:100373/1‑133 (MQ=255)                                         cGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCAt                                                                                      <  2:100373/133‑1 (MQ=255)                                         cGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGt                                                                                 >  2:329900/1‑138 (MQ=255)                                         cGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGt                                                                                 <  1:329900/138‑1 (MQ=255)                                         cGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTc                                                                                >  1:157510/1‑139 (MQ=255)                                         cGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTc                                                                                >  2:190571/1‑139 (MQ=255)                                            ggAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCAcg                                                                             >  1:1032681/1‑139 (MQ=255)                                                aTCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTa                                                                         <  2:1016810/139‑1 (MQ=255)                                                    ttCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGa                                                                                                      <  2:518887/106‑1 (MQ=255)                                                    ttCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGa                                                                                                      >  1:518887/1‑106 (MQ=255)                                                           ccTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGttt                                                                                                               >  1:401565/1‑90 (MQ=255)                                                           ccTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGttt                                                                                                               <  2:401565/90‑1 (MQ=255)                                                                    ccGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGc                                                     <  1:443990/139‑1 (MQ=255)                                                                       gATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCAt                                                                                            <  1:2047/97‑1 (MQ=255)                                                                       gATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCAt                                                                                            >  2:2047/1‑97 (MQ=255)                                                                       gATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGtt                                                  <  1:326244/139‑1 (MQ=255)                                                                        aTTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTc                                                 <  2:586243/139‑1 (MQ=255)                                                                                    gTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGc                                     >  2:499791/1‑139 (MQ=255)                                                                                     tCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCt                                    <  2:750009/139‑1 (MQ=255)                                                                                            aaGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTc                             <  2:256664/139‑1 (MQ=255)                                                                                               tttCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGc                          <  1:190571/139‑1 (MQ=255)                                                                                                   gAGGTTACCACCAGAAAACTTTGAACCGGGGTgcgc                                                                                                                             <  2:659769/36‑1 (MQ=255)                                                                                                          ccaccaGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCATCGCATTACGCTCACGTTCGGCATATTTCTTGt               <  2:780384/139‑1 (MQ=255)                                                                                                                  aaaCTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCaaa       <  2:531366/139‑1 (MQ=255)                                                                                                                     cTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGt    >  2:1098972/1‑139 (MQ=255)                                                                                                                       ttGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTc  <  2:1032681/139‑1 (MQ=255)                                                                                                                        tGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGcc                                                                   <  1:1178543/73‑1 (MQ=255)                                                                                                                        tGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGcc                                                                   >  2:1178543/1‑73 (MQ=255)                                                                                                                                    |                                                                                                                              TAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTC  >  NC_000913/2958178‑2958434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑