Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,984,021	T→C	Y35C (TAC→TGC)	kduI ←	hexuronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,984,021	0	T	C	100.0%	231.6 / NA	63	Y35C (TAC→TGC)	kduI	hexuronate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/31);  total (32/31)

ACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCGTGAAA  >  NC_000913/2983888‑2984155                                                                                                                                      |                                                                                                                                       acCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCa                                                                                                                                   >  2:756607/1‑139 (MQ=255)   cTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATc                                                                                                                                 >  2:1084027/1‑139 (MQ=255)      aCTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGc                                                                                                                              >  2:1243509/1‑139 (MQ=255)           cgACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACaa                                                                                                                         >  1:1128457/1‑139 (MQ=255)           cgACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACaa                                                                                                                         >  2:1239013/1‑139 (MQ=255)           cgACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACaa                                                                                                                         >  2:1260931/1‑139 (MQ=255)                    aGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCtttt                                                                                                                <  1:30453/139‑1 (MQ=255)                          tAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCgg                                                                                                                               >  2:1268911/1‑118 (MQ=255)                          tAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCgg                                                                                                                               <  1:1268911/118‑1 (MQ=255)                          tAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCa                                                                                                          >  2:987410/1‑139 (MQ=255)                          tAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCa                                                                                                          >  2:1280133/1‑139 (MQ=255)                             cTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGGGCACTCATCGGCGACAAATACCTTTTCAACCaaaa                                                                                                       >  1:795123/1‑139 (MQ=255)                                 cGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCTCTCATCGGCGACAAATACCTTTTCAACCAAAAATTc                                                                                                   >  1:323500/1‑139 (MQ=255)                                     gAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTg                                                                                               >  2:32649/1‑139 (MQ=255)                                       gttgttTACCAACTTCCCCGCCAACGGAAGCCGTTTTAGGTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             <  2:1050916/139‑1 (MQ=255)                                       gttgttTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCTCTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             <  2:323500/139‑1 (MQ=255)                                       gttgttTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             <  1:315402/139‑1 (MQ=255)                                       gttgttTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             <  1:756607/139‑1 (MQ=255)                                        ttgttTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcgc                                                                                            <  1:32649/139‑1 (MQ=255)                                          gttTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAg                                                                                          <  2:925428/139‑1 (MQ=255)                                            ttACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGcc                                                                                        <  2:1128457/139‑1 (MQ=255)                                                   cTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTGAACCATGGTGCACTCATCGGCGACAATTACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGt                                                                                 >  1:797950/1‑139 (MQ=255)                                                   cTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGAc                                                                                                                           <  2:1095255/97‑1 (MQ=255)                                                   cTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGAc                                                                                                                           >  1:1095255/1‑97 (MQ=255)                                                     tCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACa                                                                                                                          >  2:9061/1‑96 (MQ=255)                                                     tCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACa                                                                                                                          <  1:9061/96‑1 (MQ=255)                                                     tCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             >  2:300581/1‑125 (MQ=255)                                                     tCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             <  1:300581/125‑1 (MQ=255)                                                       cccGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATcc                                                                             >  1:267588/1‑139 (MQ=255)                                                               cGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGtttt                                                                     <  1:461177/139‑1 (MQ=255)                                                                    aCCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTtgcgt                                                                >  2:244/1‑139 (MQ=255)                                                                       gTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTtgcgtgcg                                                             <  1:649836/139‑1 (MQ=255)                                                                               tATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTgg                                                     <  1:457899/139‑1 (MQ=255)                                                                                aTCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCa                                                                                                  >  1:1252128/1‑93 (MQ=255)                                                                                aTCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCa                                                                                                  <  2:1252128/93‑1 (MQ=255)                                                                                aTCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGa                                                    >  1:299065/1‑139 (MQ=255)                                                                                 tCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGAt                                                   <  1:549351/139‑1 (MQ=255)                                                                                 tCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGAt                                                   <  2:267588/139‑1 (MQ=255)                                                                                  cGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATg                                                  <  1:1084027/139‑1 (MQ=255)                                                                                   ggcttcaTGCCGTCGACAATCATCCGGTTAATGTGGCTGTAAACCACGGTGCACTCATCGGCGACAAATACCATTTCACCCACAAAATCGTTAGGCATGACCTGGGTATACAGCGTATTTGCGTGCGCCCTGCGGacgt                                                 <  2:188290/133‑4 (MQ=255)                                                                                   ggCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGc                                                 <  1:1243509/139‑1 (MQ=255)                                                                                          tGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCa                                                                                                  >  1:1283403/1‑83 (MQ=255)                                                                                          tGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCa                                                                                                  <  2:1283403/83‑1 (MQ=255)                                                                                          tGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCt                                          <  1:1239013/139‑1 (MQ=255)                                                                                               ccAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGt                                     >  2:234203/1‑139 (MQ=255)                                                                                                         ttCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAAc                           <  2:299065/139‑1 (MQ=255)                                                                                                             gTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTcc                       >  1:546801/1‑139 (MQ=255)                                                                                                                      gCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAg              <  2:1113763/139‑1 (MQ=255)                                                                                                                      gCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAg              >  1:938907/1‑139 (MQ=255)                                                                                                                       cTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGt             >  2:834119/1‑139 (MQ=255)                                                                                                                          tAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             >  2:579141/1‑56 (MQ=255)                                                                                                                          tAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             >  2:1216958/1‑56 (MQ=255)                                                                                                                          tAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             <  1:579141/56‑1 (MQ=255)                                                                                                                          tAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             <  1:1216958/56‑1 (MQ=255)                                                                                                                          tAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGcc                                                                                        >  1:310430/1‑61 (MQ=255)                                                                                                                          tAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGcc                                                                                        <  2:310430/61‑1 (MQ=255)                                                                                                                           aaaCCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAg         <  1:234203/139‑1 (MQ=255)                                                                                                                            aaCCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTa                                                                                <  2:246099/67‑1 (MQ=255)                                                                                                                            aaCCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTa                                                                                >  1:246099/1‑67 (MQ=255)                                                                                                                                 tGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCGTGaa   >  2:735348/1‑139 (MQ=255)                                                                                                                                 tGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCGTGaa   <  1:244/139‑1 (MQ=255)                                                                                                                                  ggTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCGTGaaa  >  1:1191146/1‑139 (MQ=255)                                                                                                                                  ggTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCGTGaaa  >  1:1095404/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                       ACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCGTGAAA  >  NC_000913/2983888‑2984155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑