Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 30,616 T→C P322P (CCT→CCC)  carA → carbamoyl phosphate synthetase small subunit, glutamine amidotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091330,6160TC100.0% 114.4 / NA 32P322P (CCT→CCC) carAcarbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/12);  total (20/12)

GGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACACGACGCCGCG  >  NC_000913/30481‑30742
                                                                                                                                       |                                                                                                                              
ggcgAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCa                                                                                                                             <  2:25131/139‑1 (MQ=255)
             aaGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCa                                                                                                                <  1:981484/139‑1 (MQ=255)
                              aTTTGGTCACCACGGCGGCAGCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTc                                                                                               >  1:120864/1‑139 (MQ=255)
                                ttGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa                                                                                             <  1:249210/139‑1 (MQ=255)
                                  ggTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACg                                                                                           <  1:325500/139‑1 (MQ=255)
                                        cacGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACg                                                                                           >  1:562187/1‑133 (MQ=255)
                                        cacGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACg                                                                                           <  2:562187/133‑1 (MQ=255)
                                        cacGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGt                                                                                     >  2:473652/1‑139 (MQ=255)
                                              ggcAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAgg                                                                               <  2:1112192/139‑1 (MQ=255)
                                                       ccGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATc                                                                      >  2:1305909/1‑139 (MQ=255)
                                                       ccGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATc                                                                      >  2:925401/1‑139 (MQ=255)
                                                         ggTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGc                                                                    <  1:449702/139‑1 (MQ=255)
                                                          gTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCa                                                                   >  1:1218849/1‑139 (MQ=255)
                                                                    tgGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGtt                                                                                    <  1:709715/112‑1 (MQ=255)
                                                                    tgGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGtt                                                                                    >  2:709715/1‑112 (MQ=255)
                                                                    tgGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAAcc                                                         >  2:793914/1‑139 (MQ=255)
                                                                                           aCCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCAcc                                  >  1:840879/1‑139 (MQ=255)
                                                                                             cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGAACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCt                                >  1:824759/1‑139 (MQ=255)
                                                                                               cccTGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGATGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAg                                         <  1:806563/128‑1 (MQ=255)
                                                                                               cccAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAg                                         >  2:806563/1‑128 (MQ=255)
                                                                                               cccAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGa                              >  1:1064413/1‑139 (MQ=255)
                                                                                                 cAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAag                            >  1:1300980/1‑139 (MQ=255)
                                                                                                  aGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAagc                           >  2:548217/1‑139 (MQ=255)
                                                                                                         cGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGc                                                                    <  1:151048/91‑1 (MQ=255)
                                                                                                         cGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGc                                                                    >  2:151048/1‑91 (MQ=255)
                                                                                                               tGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTc                                                                                                                 >  1:1126332/1‑40 (MQ=255)
                                                                                                               tGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTc                                                                                                                 <  2:1126332/40‑1 (MQ=255)
                                                                                                                      gACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACacgacg       >  1:84753/1‑139 (MQ=255)
                                                                                                                      gACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACacgacg       >  1:1275329/1‑139 (MQ=255)
                                                                                                                           aGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACACGAcgccgcg  >  2:1001028/1‑139 (MQ=255)
                                                                                                                                 aTTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCAc                                                                  <  2:213662/69‑1 (MQ=255)
                                                                                                                                 aTTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCAc                                                                  >  1:213662/1‑69 (MQ=255)
                                                                                                                                       |                                                                                                                              
GGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACACGACGCCGCG  >  NC_000913/30481‑30742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑