Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,068,173	C→T	*212* (TAG→TAA)	argO ←	arginine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,068,173	0	C	T	100.0%	181.3 / NA	56	*212* (TAG→TAA)	argO	arginine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/26);  total (30/26)

TAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGTGCCAGCC  >  NC_000913/3068038‑3068303                                                                                                                                        |                                                                                                                                   tAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTaa                                                                                                                                 <  2:408562/139‑1 (MQ=255)              tGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAg                                                                                                                        >  1:702822/1‑135 (MQ=255)              tGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAg                                                                                                                        <  2:702822/135‑1 (MQ=255)              tGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCtt                                                                                                                    >  2:144565/1‑139 (MQ=255)                ccgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGAGGTAATTGACTGGATCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAAGTGAAAAAGACTtgt                                                                                                                  >  2:337525/1‑139 (MQ=255)                  gccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGc                                                                                                                <  1:694812/139‑1 (MQ=255)                  gccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGc                                                                                                                <  1:325269/139‑1 (MQ=255)                    cAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCAt                                                                                                              >  1:320374/1‑139 (MQ=255)                       ggCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAg                                                                                                           >  2:1215751/1‑139 (MQ=255)                                        tCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTaa                                                                                                                                 >  2:464144/1‑100 (MQ=255)                                        tCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTaa                                                                                                                                 <  1:464144/100‑1 (MQ=255)                                                gTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGc                                                                                                                      <  2:1264258/103‑1 (MQ=255)                                                gTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGc                                                                                                                      >  1:1264258/1‑103 (MQ=255)                                                 tctctcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAg                                                                                 >  1:117931/1‑139 (MQ=255)                                                   tctcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGG‑GGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCa                                                                              <  2:1014846/139‑1 (MQ=255)                                                      ccGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAAt                                                                            >  2:476332/1‑139 (MQ=255)                                                       cGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                                           <  1:667140/139‑1 (MQ=255)                                                             aaTTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACcac                                                                     >  2:935506/1‑139 (MQ=255)                                                                   cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAaca                                                               <  2:978483/139‑1 (MQ=255)                                                                   cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAaca                                                               <  1:144565/139‑1 (MQ=255)                                                                   cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAaca                                                               >  1:978483/1‑139 (MQ=255)                                                                    ttGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCa                                                                                   >  1:253384/1‑118 (MQ=255)                                                                    ttGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCa                                                                                   <  2:253384/118‑1 (MQ=255)                                                                        ccAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                                          >  2:511870/1‑139 (MQ=255)                                                                            aaTTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCcaca                                                      <  1:1213727/139‑1 (MQ=255)                                                                                      ctctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGa                                            >  2:685126/1‑139 (MQ=255)                                                                                       tctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACcaca                                                                    >  2:350654/1‑114 (MQ=255)                                                                                       tctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACcaca                                                                    <  1:350654/114‑1 (MQ=255)                                                                                              gCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATgcgc                                    <  1:337525/139‑1 (MQ=255)                                                                                              gCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATgcgc                                    <  1:165134/139‑1 (MQ=255)                                                                                              gCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATgcgc                                    <  2:33129/139‑1 (MQ=255)                                                                                                aaGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                                          >  2:662704/1‑115 (MQ=255)                                                                                                aaGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                                          <  1:662704/115‑1 (MQ=255)                                                                                                aaGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtg                                  >  2:892838/1‑139 (MQ=255)                                                                                                aaGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtg                                  >  2:962711/1‑139 (MQ=255)                                                                                                 aGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtgt                                 >  2:1053649/1‑139 (MQ=255)                                                                                                 aGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtgt                                 >  2:1227007/1‑139 (MQ=255)                                                                                                 aGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtgt                                 >  1:965829/1‑139 (MQ=255)                                                                                                 aGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtgt                                 >  1:611854/1‑139 (MQ=255)                                                                                                  gCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtgtg                                >  2:995527/1‑139 (MQ=255)                                                                                                   cTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAAc                                                    >  2:470810/1‑118 (MQ=255)                                                                                                   cTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAAc                                                    <  1:470810/118‑1 (MQ=255)                                                                                                          ccTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGcc                        <  2:320374/139‑1 (MQ=255)                                                                                                            tctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGt                      >  1:1151001/1‑139 (MQ=255)                                                                                                            tctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGt                      >  1:22185/1‑139 (MQ=255)                                                                                                             ctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCa                                                                              <  2:535419/82‑1 (MQ=255)                                                                                                             ctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCa                                                                              >  1:535419/1‑82 (MQ=255)                                                                                                             ctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                                              <  1:665091/98‑1 (MQ=255)                                                                                                             ctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                                              >  2:665091/1‑98 (MQ=255)                                                                                                                        ttCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGc                                                                                                                <  1:888104/37‑1 (MQ=255)                                                                                                                        ttCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGc                                                                                                                >  2:888104/1‑37 (MQ=255)                                                                                                                        ttCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                                           >  2:665057/1‑74 (MQ=255)                                                                                                                        ttCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                                           <  1:665057/74‑1 (MQ=255)                                                                                                                         tCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGt         <  1:476332/139‑1 (MQ=255)                                                                                                                                gATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGTgccagcc  <  1:962711/139‑1 (MQ=255)                                                                                                                                gATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGTgccagcc  <  2:22185/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   TAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGTGCCAGCC  >  NC_000913/3068038‑3068303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑