Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,085,461 A→G S151P (TCC→CCC)  speA ← biosynthetic arginine decarboxylase, PLP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,085,4610AG100.0% 222.6 / NA 62S151P (TCC→CCC) speAbiosynthetic arginine decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/30);  total (32/30)

CCAGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGC  >  NC_000913/3085326‑3085596
                                                                                                                                       |                                                                                                                                       
ccAGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGAcc                                                                                                                                      >  2:629765/1‑139 (MQ=255)
  aGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCgg                                                                                                                                    <  1:629765/139‑1 (MQ=255)
         ccTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAg                                                                                                                             >  2:310605/1‑139 (MQ=255)
           ttGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGAc                                                                                                                           <  1:984467/139‑1 (MQ=255)
            tgtgCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGAcc                                                                                                                          >  1:831483/1‑139 (MQ=255)
            tgtgCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGAcc                                                                                                                          >  2:1269145/1‑139 (MQ=255)
             gtgCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGAccc                                                                                                                         >  2:1070297/1‑139 (MQ=255)
              tgCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCa                                                                                                                        >  1:212924/1‑139 (MQ=255)
                       ttctCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGc                                                                                                               <  1:460287/139‑1 (MQ=255)
                           cGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCga                                                                                                           <  2:1087689/139‑1 (MQ=255)
                             ccAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCgaat                                                                                                         <  1:1121466/139‑1 (MQ=255)
                                          ggCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTc                                                                                            >  1:419710/1‑139 (MQ=255)
                                            cGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCaa                                                                                          <  1:80963/139‑1 (MQ=255)
                                               atatatTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCa                                                                                       <  2:419710/139‑1 (MQ=255)
                                                tatatTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCAc                                                                                      >  1:465252/1‑139 (MQ=255)
                                                    ttCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACgcgg                                                                                  <  1:1269145/139‑1 (MQ=255)
                                                    ttCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACgcgg                                                                                  <  1:1070297/139‑1 (MQ=255)
                                                         ggTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                             <  2:714362/139‑1 (MQ=255)
                                                         ggTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                             >  1:1033277/1‑139 (MQ=255)
                                                         ggTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                             >  2:651268/1‑139 (MQ=255)
                                                               tataACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGtt                                                                       >  2:683517/1‑139 (MQ=255)
                                                                     cGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACttt                                                                 >  1:507538/1‑139 (MQ=255)
                                                                       ttGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCga                                                                                                           <  2:680954/95‑1 (MQ=255)
                                                                       ttGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCga                                                                                                           >  1:680954/1‑95 (MQ=255)
                                                                       ttGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGa                                                               <  1:310605/139‑1 (MQ=255)
                                                                       ttGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGa                                                               <  2:212924/139‑1 (MQ=255)
                                                                       ttGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGa                                                               >  1:894864/1‑139 (MQ=255)
                                                                        tGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGAt                                                              <  2:507538/139‑1 (MQ=255)
                                                                         gCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGAt                                                              >  2:364960/1‑138 (MQ=255)
                                                                         gCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGAt                                                              <  1:364960/138‑1 (MQ=255)
                                                                         gCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATc                                                             >  1:1287375/1‑139 (MQ=255)
                                                                           aGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCgg                                                           <  1:264036/139‑1 (MQ=255)
                                                                                  gACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTg                                                                >  2:874273/1‑127 (MQ=255)
                                                                                  gACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTg                                                                <  1:874273/127‑1 (MQ=255)
                                                                                  gACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACa                                                    <  2:371806/139‑1 (MQ=255)
                                                                                    cGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGa                                                               >  1:1234678/1‑126 (MQ=255)
                                                                                    cGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGa                                                               <  2:1234678/126‑1 (MQ=255)
                                                                                         cGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCg                                                                                                                                     >  2:58556/1‑51 (MQ=255)
                                                                                         cGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCg                                                                                                                                     <  1:58556/51‑1 (MQ=255)
                                                                                         cGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGt                                             >  2:1176605/1‑139 (MQ=255)
                                                                                           ggTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACgcgg                                                                                  >  2:183957/1‑100 (MQ=255)
                                                                                           ggTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACgcgg                                                                                  <  1:183957/100‑1 (MQ=255)
                                                                                           ggTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTaa                                           >  1:1085749/1‑139 (MQ=255)
                                                                                            gTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAAt                                          <  2:1085749/139‑1 (MQ=255)
                                                                                               aTGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACt                                                                                             >  2:966481/1‑85 (MQ=255)
                                                                                               aTGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACt                                                                                             <  1:966481/85‑1 (MQ=255)
                                                                                               aTGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTaa                                           <  2:604062/135‑1 (MQ=255)
                                                                                               aTGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTaa                                           >  1:604062/1‑135 (MQ=255)
                                                                                                     gCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTtat                                 <  1:1045902/139‑1 (MQ=255)
                                                                                                      cATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTtata                                <  2:465252/139‑1 (MQ=255)
                                                                                                      cATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTtata                                >  2:96136/1‑139 (MQ=255)
                                                                                                          tgCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCg                            >  1:1077838/1‑139 (MQ=255)
                                                                                                           gCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAg                                                                                                  <  2:1189243/68‑1 (MQ=255)
                                                                                                           gCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAg                                                                                                  >  1:1189243/1‑68 (MQ=255)
                                                                                                           gCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                             >  2:106508/1‑89 (MQ=255)
                                                                                                           gCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                             <  1:106508/89‑1 (MQ=255)
                                                                                                                    gCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                             >  2:175696/1‑80 (MQ=255)
                                                                                                                    gCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                             <  1:175696/80‑1 (MQ=255)
                                                                                                                     ccATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCt                 >  1:239947/1‑139 (MQ=255)
                                                                                                                               ggCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTg       >  2:516173/1‑139 (MQ=255)
                                                                                                                               ggCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTg       >  2:745176/1‑139 (MQ=255)
                                                                                                                                    tGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAAcgc  <  1:651268/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CCAGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGC  >  NC_000913/3085326‑3085596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑