Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,106,905	T→C	F398L (TTC→CTC)	nupG →	nucleoside transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,106,905	0	T	C	100.0%	215.9 / NA	59	F398L (TTC→CTC)	nupG	nucleoside transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/35);  total (24/35)

TAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGT  >  NC_000913/3106772‑3107039                                                                                                                                      |                                                                                                                                       tAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATg                                                                                                                                   <  2:642838/139‑1 (MQ=255)  aaCGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATgg                                                                                                                                  <  1:518619/139‑1 (MQ=255)    cGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                <  2:599840/139‑1 (MQ=255)    cGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                <  2:4442/139‑1 (MQ=255)    cGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                <  1:45586/139‑1 (MQ=255)    cGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACAGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                <  1:427330/139‑1 (MQ=255)         tCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGtt                                                                                                                           >  1:684519/1‑139 (MQ=255)          cGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTc                                                                                                                          <  1:991926/139‑1 (MQ=255)                tATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                >  2:920495/1‑127 (MQ=255)                tATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                <  1:920495/127‑1 (MQ=255)                  tCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATaa                                                                                                                  <  2:684519/139‑1 (MQ=255)                            cATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACTGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAat                                                                                                                      <  1:706275/125‑1 (MQ=255)                            cATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCaa                                                                                                                        <  1:242619/123‑1 (MQ=255)                            cATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCaa                                                                                                                        >  2:242619/1‑123 (MQ=255)                            cATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAat                                                                                                                      >  2:706275/1‑125 (MQ=255)                                  gAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCg                                                                                                  <  2:298930/139‑1 (MQ=255)                                             ttgttgATATGTACACCCCAAACGGCATTACCGACTGGCAGACGGTATGGTTGCTTTTTGCTGGTTAATCCGAGGTTCTGGCCTTGGCGCTCATGGCGATGTTCAAATATAAACGCGTTCGTGTCCCGACAGGcacaca                                                                                       <  2:1274691/139‑1 (MQ=255)                                             ttgttgAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGcacaca                                                                                       <  1:798263/139‑1 (MQ=255)                                                ttgAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGAc                                                                                    >  2:376786/1‑139 (MQ=255)                                                   agaTGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGt                                                                                 <  1:1086130/139‑1 (MQ=255)                                                          cacCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCAc                                                                          >  2:681044/1‑139 (MQ=255)                                                             ccAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                <  1:410422/82‑1 (MQ=255)                                                             ccAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                >  2:410422/1‑82 (MQ=255)                                                               aaaaCGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCa                                                                                                                         <  2:1067202/87‑1 (MQ=255)                                                               aaaaCGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCa                                                                                                                         >  1:1067202/1‑87 (MQ=255)                                                                aaaCGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTa                                                                    >  2:1269052/1‑139 (MQ=255)                                                                  aCGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACg                                                                  <  2:825955/139‑1 (MQ=255)                                                                       aTTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTc                                                                                                                          <  2:22743/78‑1 (MQ=255)                                                                       aTTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTc                                                                                                                          >  1:22743/1‑78 (MQ=255)                                                                                 ggCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGt                                                   <  2:616801/139‑1 (MQ=255)                                                                                      aCCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCa                                              >  1:1121996/1‑139 (MQ=255)                                                                                      aCCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCa                                              >  2:1267053/1‑139 (MQ=255)                                                                                          tATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                <  2:1040269/53‑1 (MQ=255)                                                                                          tATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCg                                                                                                                                >  1:1040269/1‑53 (MQ=255)                                                                                                   ttttCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGt                                 <  2:1121996/139‑1 (MQ=255)                                                                                                   ttttCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGt                                 <  1:1269052/139‑1 (MQ=255)                                                                                                   ttttCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGt                                 <  1:1267053/139‑1 (MQ=255)                                                                                                    tttCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGtt                                                                                                                           <  2:74435/48‑1 (MQ=255)                                                                                                    tttCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGtt                                                                                                                           >  1:74435/1‑48 (MQ=255)                                                                                                        gCTGGTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGtttttt                            >  2:502358/1‑139 (MQ=255)                                                                                                            gTTACTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTAt                        >  1:339059/1‑139 (MQ=255)                                                                                                               aCTCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCt                     <  2:339059/139‑1 (MQ=255)                                                                                                                 tCCGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTa                   >  2:288404/1‑139 (MQ=255)                                                                                                                  ccGTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTAc                  >  2:60281/1‑139 (MQ=255)                                                                                                                    gTGGTTCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACtt                <  1:376786/139‑1 (MQ=255)                                                                                                                        ttCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAac            >  2:1260633/1‑139 (MQ=255)                                                                                                                         tCTGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAaca           <  1:60281/139‑1 (MQ=255)                                                                                                                           tGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGtttttttt                          >  2:569181/1‑122 (MQ=255)                                                                                                                           tGGCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGtttttttt                          <  1:569181/122‑1 (MQ=255)                                                                                                                            ggCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACAt        <  2:1066250/139‑1 (MQ=255)                                                                                                                            ggCCTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACAt        <  1:1260633/139‑1 (MQ=255)                                                                                                                              ccTTCGCGCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATaa      <  1:288404/139‑1 (MQ=255)                                                                                                                                  cgcgCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGt                                 >  2:348044/1‑108 (MQ=255)                                                                                                                                  cgcgCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGt                                 <  1:348044/108‑1 (MQ=255)                                                                                                                                  cgcgCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTTTTACTTCa              >  2:246576/1‑127 (MQ=255)                                                                                                                                  cgcgCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCa              <  1:246576/127‑1 (MQ=255)                                                                                                                                  cgcgCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCa              >  1:700684/1‑127 (MQ=255)                                                                                                                                  cgcgCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGt  >  2:830930/1‑139 (MQ=255)                                                                                                                                  cgcgCTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACAAAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCa              <  2:700684/127‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       TAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGT  >  NC_000913/3106772‑3107039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑