Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,115,428	T→C	T1229A (ACC→GCC)	sslE ←	putative secreted and surface‑associated lipoprotein mucinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,115,428	0	T	C	100.0%	250.5 / NA	67	T1229A (ACC→GCC)	sslE	putative secreted and surface‑associated lipoprotein mucinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/33);  total (34/33)

CGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGTATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCGTCTGATTCCAGCTCACCCAGCGGAGCCGGTGAGTTCAGA  >  NC_000913/3115320‑3115552                                                                                                             |                                                                                                                             cGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTcc                                                                                                >  1:455130/1‑139 (MQ=255)   ttGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCg                                                                                               <  1:1041775/138‑1 (MQ=255)   ttGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCg                                                                                               >  2:1041775/1‑138 (MQ=255)   ttGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGc                                                                                              >  2:952721/1‑139 (MQ=255)   ttGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGc                                                                                              <  2:725279/139‑1 (MQ=255)   ttGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGc                                                                                              <  2:1250345/139‑1 (MQ=255)   ttGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGc                                                                                              <  1:29925/139‑1 (MQ=255)    tGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGcc                                                                                             >  2:157024/1‑139 (MQ=255)           aCGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTaa                                                                                      >  1:764059/1‑139 (MQ=255)                gTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTc                                                                                                      >  2:902719/1‑118 (MQ=255)                gTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTc                                                                                                      <  1:902719/118‑1 (MQ=255)                gTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGc                                                                                              <  1:1175821/126‑1 (MQ=255)                gTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGc                                                                                              >  2:1175821/1‑126 (MQ=255)                  ttGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTgg                                                                               <  1:499247/139‑1 (MQ=255)                   tgtgGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGc                                                                                  >  1:106001/1‑135 (MQ=255)                   tgtgGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGc                                                                                  <  2:106001/135‑1 (MQ=255)                     tgGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTc                                                                                                      <  2:125368/113‑1 (MQ=255)                     tgGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTc                                                                                                      >  1:125368/1‑113 (MQ=255)                     tgGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCAt                                                                            <  2:26405/139‑1 (MQ=255)                         ccGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCg                                                                                                              <  2:730673/101‑1 (MQ=255)                         ccGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCg                                                                                                              >  1:730673/1‑101 (MQ=255)                           ggCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGt                                                                      >  2:822580/1‑139 (MQ=255)                                    ttATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGTATTCAGGTTCTTCTTCg                                                             <  1:822580/139‑1 (MQ=255)                                    ttATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCg                                                             <  1:1206266/139‑1 (MQ=255)                                       taGGTAAACATCCGGTGCGTACCGTCGTCGCTATCACGGCCGTAGAAGGCATTCATCGAGCTGCAAAAGGCATCCAGATCGTTAGCGAATTGCTGCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGgtg                                                          <  2:564633/139‑1 (MQ=255)                                                    ggTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTTATTCATCGAGCTGGCAAAGGCATCCAGAt                                                                                                                      <  2:758553/66‑1 (MQ=255)                                                    ggTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGAt                                                                                                                      >  1:758553/1‑66 (MQ=255)                                                    ggTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGaa                                             >  2:1027593/1‑139 (MQ=255)                                                    ggTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGaa                                             >  1:38066/1‑139 (MQ=255)                                                        cTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCAGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCg                                         >  1:699195/1‑139 (MQ=255)                                                        cTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCACCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCg                                         >  2:939473/1‑139 (MQ=255)                                                          tACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAg                                                                                                   <  2:433449/79‑1 (MQ=255)                                                          tACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAg                                                                                                   >  1:433449/1‑79 (MQ=255)                                                          tACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCGTc                                       >  1:690342/1‑139 (MQ=255)                                                           aCCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCt                                                                                 >  2:1288557/1‑96 (MQ=255)                                                           aCCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCt                                                                                 <  1:1288557/96‑1 (MQ=255)                                                           aCCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCGTCt                                      >  1:1074822/1‑139 (MQ=255)                                                               tCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCGTCTGAtt                                  <  2:455130/139‑1 (MQ=255)                                                               tCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCGTCTGAtt                                  <  1:157024/139‑1 (MQ=255)                                                                cTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCt                                                                                 >  2:581461/1‑91 (MQ=255)                                                                cTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCt                                                                                 <  1:581461/91‑1 (MQ=255)                                                                  tCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGttcttctt                                                               >  2:893862/1‑107 (MQ=255)                                                                  tCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGttcttctt                                                               <  1:893862/107‑1 (MQ=255)                                                                         cACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCGTCTGATTCCAGCTCAcc                        >  1:865807/1‑139 (MQ=255)                                                                             gCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCg                                                             >  1:1180734/1‑98 (MQ=255)                                                                             gCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCg                                                             <  2:1180734/98‑1 (MQ=255)                                                                             gCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGgtg                                                          >  2:1110118/1‑101 (MQ=255)                                                                             gCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGgtg                                                          <  1:1110118/101‑1 (MQ=255)                                                                             gCCGTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCGTCTGATTCCAGCTCACCCAGc                    >  1:1226377/1‑139 (MQ=255)                                                                                gTAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCGTCTGATTCCAGCTCACCCAGCGGa                 >  2:539191/1‑139 (MQ=255)                                                                                 tAGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCGTCTGATTCCAGCTCACCCAGCGGAg                <  2:1226377/139‑1 (MQ=255)                                                                                  aGAAGTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCGTCTGATTCCAGCTCACCCAGCGGAGc               >  2:1284138/1‑139 (MQ=255)                                                                                      gTCATTCATCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCGTCTGATTCCAGCTCACCCAGCGGAGCCGGt           <  1:1284138/139‑1 (MQ=255)                                                                                        cattcatCGAGCTGGCAAAGGCATCCAGATCGTTAGCGaa                                                                                                            <  2:905034/40‑1 (MQ=255)                                                                                        cattcatCGAGCTGGCAAAGGCATCCAGATCGTTAGCGaa                                                                                                            >  1:905034/1‑40 (MQ=255)                                                                                         attcatCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGgtg                                                          >  1:34850/1‑89 (MQ=255)                                                                                         attcatCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGgtg                                                          <  2:34850/89‑1 (MQ=255)                                                                                         attcatCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCGTCTGATTCCAGCTCACCCAGCGGAGCCGGTGAg        >  1:1282992/1‑139 (MQ=255)                                                                                          ttcatCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAg                                           <  2:303936/103‑1 (MQ=255)                                                                                          ttcatCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAg                                           >  1:303936/1‑103 (MQ=255)                                                                                             atCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCGTCTGATTCCAGCTCACCCAGCGGAGCCGGTGAGTTCa    <  2:1074822/139‑1 (MQ=255)                                                                                              tCGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCGTCTGATTCCAGCTCACCCAGCGGAGCCGGTGAGTTCAg   <  2:699195/139‑1 (MQ=255)                                                                                               cGAGCTGGCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCGTCTGATTCCAGCTCACCCAGCGGAGCCGGTGAGTTCAGa  <  1:952721/139‑1 (MQ=255)                                                                                                     ggCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCAt                                                                            <  1:719332/59‑1 (MQ=255)                                                                                                     ggCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCAt                                                                            >  2:719332/1‑59 (MQ=255)                                                                                                     ggCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCGTCTGATTCCAGCTCACCCAGCGGAg                >  2:1071265/1‑119 (MQ=255)                                                                                                     ggCAAAGGCATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCGTCTGATTCCAGCTCACCCAGCGGAg                <  1:1071265/119‑1 (MQ=255)                                                                                                             |                                                                                                                             CGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGTATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGCTGGCATTCAGGTTCTTCTTCGGTGTGGTATAGACGAAAGCGTCTGATTCCAGCTCACCCAGCGGAGCCGGTGAGTTCAGA  >  NC_000913/3115320‑3115552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑