Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,116,871 C→T A748T (GCA→ACA)  sslE ← putative secreted and surface‑associated lipoprotein mucinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,116,8710CT100.0% 211.5 / NA 65A748T (GCA→ACA) sslEputative secreted and surface‑associated lipoprotein mucinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/30);  total (35/30)

ACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTC  >  NC_000913/3116736‑3117004
                                                                                                                                       |                                                                                                                                     
aCGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGc                                                                                                                                    >  1:830945/1‑139 (MQ=255)
  ggCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCAt                                                                                                                                  >  1:204808/1‑139 (MQ=255)
       cgcACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAAc                                                                                                                             >  1:437380/1‑139 (MQ=255)
       cgcACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGATGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAAc                                                                                                                             >  2:51330/1‑139 (MQ=255)
         cACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAg                                                                                                                           >  1:1290330/1‑139 (MQ=255)
               cTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGcac                                                                                                                     <  1:51330/139‑1 (MQ=255)
                    gCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAAc                                                                                                                <  2:437380/139‑1 (MQ=255)
                       ggATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAg                                                                                                             <  2:291179/139‑1 (MQ=255)
                        gATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGa                                                                                                            >  1:933700/1‑139 (MQ=255)
                        gATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGa                                                                                                            >  1:537594/1‑139 (MQ=255)
                          tAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGAcg                                                                                                          <  2:1290330/139‑1 (MQ=255)
                                 cATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGctctc                                                                                                   <  2:814675/139‑1 (MQ=255)
                                 cATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGctctc                                                                                                   <  2:537594/139‑1 (MQ=255)
                                 cATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGctctc                                                                                                   <  1:496485/139‑1 (MQ=255)
                                  aTAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGctctct                                                                                                  >  1:654553/1‑139 (MQ=255)
                                           aaTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGctctc                                                                                                   <  2:1084553/129‑1 (MQ=255)
                                           aaTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGctctc                                                                                                   >  1:1084553/1‑129 (MQ=255)
                                                    tGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcatc                                                                                >  2:591316/1‑139 (MQ=255)
                                                      cgcgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGctctc                                                                                                   >  1:82223/1‑118 (MQ=255)
                                                      cgcgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGctctc                                                                                                   <  2:82223/118‑1 (MQ=255)
                                                      cgcgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCAc                                                                              >  1:278199/1‑139 (MQ=255)
                                                                     cgcgGTTCGGATACCCTTGCGGATCGTTATTTACTACGGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCATACGCGATCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGAt                                                                  <  1:879608/136‑1 (MQ=255)
                                                                     cgcgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGAt                                                                  >  2:879608/1‑136 (MQ=255)
                                                                       cgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCa                                                             >  1:999933/1‑139 (MQ=255)
                                                                        ggTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCAc                                                            <  1:262496/139‑1 (MQ=255)
                                                                                 aCCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCTCAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCAcc                                                   <  2:767277/139‑1 (MQ=255)
                                                                                 aCCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCAcc                                                   <  2:204808/139‑1 (MQ=255)
                                                                                 aCCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCAcc                                                   <  1:712386/139‑1 (MQ=255)
                                                                                  cccTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGTCAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcatc                                                                                >  2:927069/1‑109 (MQ=255)
                                                                                  cccTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcatc                                                                                <  1:927069/109‑1 (MQ=255)
                                                                                    cTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCttt                                                >  1:189863/1‑139 (MQ=255)
                                                                                      tGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGctct                                                                                                    >  1:759818/1‑85 (MQ=255)
                                                                                      tGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGctct                                                                                                    <  2:759818/85‑1 (MQ=255)
                                                                                      tGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGt                                              >  1:1048739/1‑139 (MQ=255)
                                                                                      tGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGt                                              <  2:999933/139‑1 (MQ=255)
                                                                                            tCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGAt                                        <  2:933700/139‑1 (MQ=255)
                                                                                             cGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATa                                       >  1:135759/1‑139 (MQ=255)
                                                                                                       tacCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcatc                                                                                >  2:1097260/1‑88 (MQ=255)
                                                                                                       tacCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcatc                                                                                <  1:1097260/88‑1 (MQ=255)
                                                                                                       tacCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATc                                                       >  2:489534/1‑113 (MQ=255)
                                                                                                       tacCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATc                                                       <  1:489534/113‑1 (MQ=255)
                                                                                                       tacCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGa                             >  1:1076116/1‑139 (MQ=255)
                                                                                                        acCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGAt                            <  2:1048739/139‑1 (MQ=255)
                                                                                                          cGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAg                                                                                                                           >  1:447238/1‑42 (MQ=255)
                                                                                                          cGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAg                                                                                                                           <  2:447238/42‑1 (MQ=255)
                                                                                                          cGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGCCGCGctct                                                                                                    >  2:230403/1‑65 (MQ=255)
                                                                                                          cGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGAcg                                                                                                          <  1:696141/59‑1 (MQ=255)
                                                                                                          cGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGAcg                                                                                                          >  2:696141/1‑59 (MQ=255)
                                                                                                          cGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGctct                                                                                                    <  1:230403/65‑1 (MQ=255)
                                                                                                            aCTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGAt                                        >  1:431759/1‑123 (MQ=255)
                                                                                                            aCTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGAt                                        <  2:431759/123‑1 (MQ=255)
                                                                                                            aCTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGt                        >  1:153164/1‑139 (MQ=255)
                                                                                                            aCTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGt                        >  1:1274184/1‑139 (MQ=255)
                                                                                                                 ttCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACAt                   <  2:612792/139‑1 (MQ=255)
                                                                                                                    aGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCct                <  1:591316/139‑1 (MQ=255)
                                                                                                                           tCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGctct                                                                                                    >  2:454665/1‑48 (MQ=255)
                                                                                                                           tCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGctct                                                                                                    <  1:454665/48‑1 (MQ=255)
                                                                                                                           tCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGttt                                                                          <  2:747054/74‑1 (MQ=255)
                                                                                                                           tCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGttt                                                                          >  1:747054/1‑74 (MQ=255)
                                                                                                                           tCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGt         >  1:725497/1‑139 (MQ=255)
                                                                                                                           tCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGt         >  1:1055461/1‑139 (MQ=255)
                                                                                                                           tCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGt         >  2:634164/1‑139 (MQ=255)
                                                                                                                                  aCCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATc                                <  1:449195/109‑1 (MQ=255)
                                                                                                                                  aCCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATc                                >  2:449195/1‑109 (MQ=255)
                                                                                                                                  aCCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTc  >  1:656410/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                     
ACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTC  >  NC_000913/3116736‑3117004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑