Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,176,721 G→A T38I (ACC→ATC)  cpdA ← 3',5' cAMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,176,7210GA100.0% 131.4 / NA 41T38I (ACC→ATC) cpdA3',5' cAMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/21);  total (20/21)

CTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCAC  >  NC_000913/3176586‑3176853
                                                                                                                                       |                                                                                                                                    
cTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGatgt                                                                                                                                   <  1:196562/139‑1 (MQ=255)
  tGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGttt                                                                                                                                 <  1:1092615/139‑1 (MQ=255)
     gATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTAcc                                                                                                                              <  2:547544/139‑1 (MQ=255)
     gATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTAcc                                                                                                                              <  2:637517/139‑1 (MQ=255)
       tGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTAcccc                                                                                                                            >  1:815804/1‑139 (MQ=255)
               gCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAggg                                                                                                                    <  2:815804/139‑1 (MQ=255)
                        tGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCt                                                                                                           <  1:724751/139‑1 (MQ=255)
                                cGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGatgt                                                                                                                                   <  2:59341/107‑1 (MQ=255)
                                cGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGatgt                                                                                                                                   >  1:59341/1‑107 (MQ=255)
                                        aTTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTg                                                                                           >  2:1143172/1‑139 (MQ=255)
                                                 gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAg                                                                                                                      <  1:725079/103‑1 (MQ=255)
                                                 gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAg                                                                                                                      >  2:725079/1‑103 (MQ=255)
                                                 gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGt                                                                                  >  2:549522/1‑139 (MQ=255)
                                                 gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGt                                                                                  >  2:925447/1‑139 (MQ=255)
                                                 gcgaTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTTACTCTCCCAGATGTTTAGCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGt                                                                                  >  2:844900/1‑139 (MQ=255)
                                                    cTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAAt                                                                               >  2:701041/1‑139 (MQ=255)
                                                       aaTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGatgt                                                                                                                                   >  1:128594/1‑84 (MQ=255)
                                                       aaTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGatgt                                                                                                                                   <  2:128594/84‑1 (MQ=255)
                                                       aaTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTg                                                                            >  1:177820/1‑139 (MQ=255)
                                                                   cgACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCaa                                                                                                  <  2:133713/105‑1 (MQ=255)
                                                                   cgACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCaa                                                                                                  >  1:133713/1‑105 (MQ=255)
                                                                   cgACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGAc                                                                >  2:503334/1‑139 (MQ=255)
                                                                    gACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGAct                                                               <  1:991691/139‑1 (MQ=255)
                                                                          cAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGcc                                                         >  1:392605/1‑139 (MQ=255)
                                                                            ggTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTc                                                       >  1:1299756/1‑139 (MQ=255)
                                                                                  aTTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccagc                                                 >  2:841276/1‑139 (MQ=255)
                                                                                  aTTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccagc                                                 <  2:177820/139‑1 (MQ=255)
                                                                                   ttCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccagcc                                                <  1:552588/139‑1 (MQ=255)
                                                                                        gCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAgg                                           <  1:1143172/139‑1 (MQ=255)
                                                                                         cTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGa                                          <  1:844900/139‑1 (MQ=255)
                                                                                                      aTCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccagcca                                               <  1:330684/121‑1 (MQ=255)
                                                                                                      aTCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccagcca                                               >  2:330684/1‑121 (MQ=255)
                                                                                                      aTCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGc                             >  1:1122626/1‑139 (MQ=255)
                                                                                                      aTCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGc                             >  2:630546/1‑139 (MQ=255)
                                                                                                       tCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGc                                                                                                    >  2:1239994/1‑67 (MQ=255)
                                                                                                       tCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGc                                                                                                    <  1:1239994/67‑1 (MQ=255)
                                                                                                                gCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATg                   <  2:392605/139‑1 (MQ=255)
                                                                                                                 cACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATgg                  <  2:1122626/139‑1 (MQ=255)
                                                                                                                  aCCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGgt                 <  2:35820/139‑1 (MQ=255)
                                                                                                                    cGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAggg                                                                                                                    >  2:16370/1‑38 (MQ=255)
                                                                                                                    cGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAggg                                                                                                                    <  1:16370/38‑1 (MQ=255)
                                                                                                                      ccTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTcc             >  2:670064/1‑139 (MQ=255)
                                                                                                                                ctcCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGa                                          <  2:231339/100‑1 (MQ=255)
                                                                                                                                ctcCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGa                                          >  1:231339/1‑100 (MQ=255)
                                                                                                                                 tcCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCac  <  2:1299756/139‑1 (MQ=255)
                                                                                                                                 tcCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCac  <  1:670064/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                    
CTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCAC  >  NC_000913/3176586‑3176853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑