Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	309,358	C→T	*223* (TAG→TAA)	ecpB ←	ECP production pilus chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	309,358	0	C	T	100.0%	142.5 / NA	44	*223* (TAG→TAA)	ecpB	ECP production pilus chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/22);  total (22/22)

GCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTT  >  NC_000913/309230‑309483                                                                                                                                 |                                                                                                                              gcgcACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACgg                                                                                                                     >  2:880626/1‑139 (MQ=255)  cgcACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAGCAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACggg                                                                                                                    >  1:600901/1‑139 (MQ=255)  cgcACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACggg                                                                                                                    >  2:858710/1‑139 (MQ=255)  cgcACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACggg                                                                                                                    >  2:1215364/1‑139 (MQ=255)   gcACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAGCAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGa                                                                                                                   <  2:600901/139‑1 (MQ=255)      cTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATg                                                                                                                <  1:1215364/139‑1 (MQ=255)                 agcGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCAcc                                                                                                     >  1:105723/1‑139 (MQ=255)                   cgcTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCt                                                                                                   >  2:1033713/1‑139 (MQ=255)                   cgcTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCt                                                                                                   >  1:395255/1‑139 (MQ=255)                           tCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGt                                                                                           <  2:38352/139‑1 (MQ=255)                                  gaacgaacAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAAccc                                                                                    >  2:1233313/1‑139 (MQ=255)                                  gaacgaacAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAAccc                                                                                    <  1:880626/139‑1 (MQ=255)                                         cAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCttt                                                                             <  2:105723/139‑1 (MQ=255)                                                 ccATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCg                                                                     <  2:395255/139‑1 (MQ=255)                                                 ccATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCg                                                                     <  1:1033713/139‑1 (MQ=255)                                                  cATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGc                                                                    >  1:807163/1‑139 (MQ=255)                                                    tGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAg                                                                  >  2:183782/1‑139 (MQ=255)                                                    tGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAg                                                                  >  2:1078993/1‑139 (MQ=255)                                                          aGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCa                                                            >  1:1213892/1‑139 (MQ=255)                                                                      cTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGa                                                                                                                   >  1:1259761/1‑72 (MQ=255)                                                                      cTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGa                                                                                                                   <  2:1259761/72‑1 (MQ=255)                                                                      cTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCAcc                                                                                                     >  1:757935/1‑86 (MQ=255)                                                                      cTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTCATCCATCTGCACAGCCATTTATTTCACGGGAATGAACTTATCAcc                                                                                                     <  2:757935/86‑1 (MQ=255)                                                                              ccTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGAc                                        >  2:733429/1‑139 (MQ=255)                                                                                  gggAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGc                                    <  1:858710/139‑1 (MQ=255)                                                                                   ggAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGcc                                   <  1:41924/139‑1 (MQ=255)                                                                                       aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCaa                                                                                       <  2:172994/83‑1 (MQ=255)                                                                                       aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCaa                                                                                       >  1:172994/1‑83 (MQ=255)                                                                                       aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGcc                                   <  1:1221893/135‑1 (MQ=255)                                                                                       aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGcc                                   >  2:1221893/1‑135 (MQ=255)                                                                                       aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGc                               >  1:341115/1‑139 (MQ=255)                                                                                        aCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCa                              <  1:1078993/139‑1 (MQ=255)                                                                                        aCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCa                              <  2:971752/139‑1 (MQ=255)                                                                                        aCCGTCGTAAAGGCATTGTCGTTAGCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCa                              <  1:733429/139‑1 (MQ=255)                                                                                          cgtcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATc                            <  1:1233313/139‑1 (MQ=255)                                                                                            tcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGaa                                                                                                              >  2:1290293/1‑55 (MQ=255)                                                                                            tcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGaa                                                                                                              <  1:1290293/55‑1 (MQ=255)                                                                                            tcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTa                                                                         >  2:155161/1‑92 (MQ=255)                                                                                            tcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTa                                                                         <  1:155161/92‑1 (MQ=255)                                                                                                             ttATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTat         <  2:1213892/139‑2 (MQ=255)                                                                                                             ttATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTac         <  1:542833/139‑1 (MQ=255)                                                                                                               aTCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCAt                             <  2:1219842/117‑1 (MQ=255)                                                                                                               aTCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCAt                             >  1:1219842/1‑117 (MQ=255)                                                                                                                    tCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCtt  >  2:864208/1‑139 (MQ=255)                                                                                                                             ctatttatTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCAc                                                           <  2:640422/73‑1 (MQ=255)                                                                                                                             ctatttatTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCAc                                                           >  1:640422/1‑73 (MQ=255)                                                                                                                                 |                                                                                                                              GCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTT  >  NC_000913/309230‑309483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑