Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,183,006	G→A	G153S (GGT→AGT)	zupT →	zinc transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,183,006	0	G	A	100.0%	107.0 / NA	35	G153S (GGT→AGT)	zupT	zinc transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/20);  total (15/20)

AGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGATTTCCC  >  NC_000913/3182873‑3183141                                                                                                                                      |                                                                                                                                        aGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGTATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCtg                                                                                                                                    >  2:1183378/1‑139 (MQ=255)    cATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGTATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCtggca                                                                                                                                 <  1:1183378/139‑1 (MQ=255)        cTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCtggcagtgg                                                                                                                             <  1:1134041/139‑1 (MQ=255)        cTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCtggcagtgg                                                                                                                             <  1:1134042/139‑1 (MQ=255)              aCTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGcc                                                                                                                       <  1:131276/139‑1 (MQ=255)               ctctCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCtggcagtgg                                                                                                                             <  1:150147/132‑1 (MQ=255)               ctctCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCtggcagtgg                                                                                                                             >  2:150147/1‑132 (MQ=255)                          aGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCgg                                                                                                           >  2:574237/1‑139 (MQ=255)                               gCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACg                                                                                                      <  2:230812/139‑1 (MQ=255)                                      ttCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTa                                                                                               >  2:1029340/1‑139 (MQ=255)                                      ttCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTa                                                                                               >  2:988898/1‑139 (MQ=255)                                      ttCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTa                                                                                               >  2:408316/1‑139 (MQ=255)                                          cGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACg                                                                                           <  2:559329/139‑1 (MQ=255)                                          cGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACg                                                                                           >  1:503841/1‑139 (MQ=255)                                                            ttGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGc                                                                         <  2:1224543/139‑1 (MQ=255)                                                               tCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCggg                                                                      <  1:1029340/139‑1 (MQ=255)                                                                    gCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGAttt                                                                 >  1:1272247/1‑139 (MQ=255)                                                                                      cTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGa                                                                                   <  2:200638/103‑1 (MQ=255)                                                                                      cTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGa                                                                                   >  1:200638/1‑103 (MQ=255)                                                                                         ggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCg                                                                                                                     >  1:1094530/1‑66 (MQ=255)                                                                                         ggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCg                                                                                                                     <  2:1094530/66‑1 (MQ=255)                                                                                            tttGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtggtg                                         <  1:988898/139‑1 (MQ=255)                                                                                            tttGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtggtg                                         <  1:408316/139‑1 (MQ=255)                                                                                               ggCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGgtggtg                                      >  1:1111216/1‑139 (MQ=255)                                                                                                 cATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGtgtg                                    <  1:574237/139‑1 (MQ=255)                                                                                                 cATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGtgtg                                    <  1:328463/139‑1 (MQ=255)                                                                                                     gCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTgg                                <  2:1272247/139‑1 (MQ=255)                                                                                                      cACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGc                               <  2:503841/139‑1 (MQ=255)                                                                                                           gCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGt                          >  1:409106/1‑139 (MQ=255)                                                                                                                  cgTTTCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCt                   <  2:596704/139‑1 (MQ=255)                                                                                                                               cccTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGAtt      >  1:1224036/1‑139 (MQ=255)                                                                                                                                 cTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTgg                                <  2:374474/111‑1 (MQ=255)                                                                                                                                 cTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTgg                                >  1:374474/1‑111 (MQ=255)                                                                                                                                   gAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGATTTccc  <  2:1111216/139‑1 (MQ=255)                                                                                                                                   gAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGATTTccc  >  1:517672/1‑139 (MQ=255)                                                                                                                                   gAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGATTTccc  <  2:498704/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        AGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGATTTCCC  >  NC_000913/3182873‑3183141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑