Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,201,206	G→A	intergenic (‑241/‑1)	ygiF ← / → ygiM	inorganic triphosphatase/SH3 domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,201,206	0	G	A	100.0%	223.4 / NA	69	intergenic (‑241/‑1)	ygiF/ygiM	inorganic triphosphatase/SH3 domain protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/32);  total (37/32)

GTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTGATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGATCATTATC  >  NC_000913/3201085‑3201339                                                                                                                            |                                                                                                                                      gTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCt                                                                                                                      >  2:374836/1‑139 (MQ=255)           gAAGCAGTGGCGAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTa                                                                                                            >  2:1161661/1‑139 (MQ=255)                             gACACAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGcc                                                                                                                                  >  1:534159/1‑99 (MQ=255)                             gACACAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGcc                                                                                                                                  <  2:534159/99‑1 (MQ=255)                              acacAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAAc                                                                                                          >  2:1260753/1‑122 (MQ=255)                              acacAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAAc                                                                                                          <  1:1260753/122‑1 (MQ=255)                                 caGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGa                                                                                      <  1:1260404/139‑1 (MQ=255)                                   gagGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGAt                                                                                                                   >  1:927101/1‑108 (MQ=255)                                   gagGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACATTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGAt                                                                                                                   <  2:927101/108‑1 (MQ=255)                                    agGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGAt                                                                                                                   >  2:34493/1‑109 (MQ=255)                                    agGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGAt                                                                                                                   <  1:34493/109‑1 (MQ=255)                                    agGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGcc                                                                                                                                  >  1:139838/1‑92 (MQ=255)                                    agGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGcc                                                                                                                                  <  2:139838/92‑1 (MQ=255)                                    agGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGAt                                                                                                                   <  1:1104922/107‑1 (MQ=255)                                    agGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGAt                                                                                                                   >  2:1104922/1‑107 (MQ=255)                                    agGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTg                                                                                   <  1:32153/139‑1 (MQ=255)                                    agGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTg                                                                                   >  1:357145/1‑139 (MQ=255)                                    agGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTg                                                                                   >  1:1012949/1‑139 (MQ=255)                                        ttCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGt                                                                               >  1:1070219/1‑139 (MQ=255)                                         tCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGtc                                                                              >  2:131512/1‑139 (MQ=255)                                            gATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           <  2:1070219/139‑1 (MQ=255)                                                           cTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAg                                                                                           >  1:372008/1‑108 (MQ=255)                                                           cTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAg                                                                                           <  2:372008/108‑1 (MQ=255)                                                           cTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           >  1:358796/1‑124 (MQ=255)                                                           cTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           <  2:358796/124‑1 (MQ=255)                                                              tGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACgcgc                                                         <  1:374836/139‑1 (MQ=255)                                                                cGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTcaca                                                                         >  1:457539/1‑121 (MQ=255)                                                                cGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTcaca                                                                         <  2:457539/121‑1 (MQ=255)                                                                          cTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGa                                                                                                               >  1:1019756/1‑73 (MQ=255)                                                                          cTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGa                                                                                                               <  2:1019756/73‑1 (MQ=255)                                                                          cTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCcgac                                             <  1:131512/139‑1 (MQ=255)                                                                           tGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCcgacg                                            <  2:1101438/139‑1 (MQ=255)                                                                             aTGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGaa                                          <  1:1161661/139‑1 (MQ=255)                                                                             aTGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGaa                                          >  2:839260/1‑139 (MQ=255)                                                                             aTGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGaa                                          <  1:767606/139‑1 (MQ=255)                                                                                    actactATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCcgacg                                            >  2:26790/1‑132 (MQ=255)                                                                                    actactATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCcgacg                                            <  1:26790/132‑1 (MQ=255)                                                                                       actaTCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGa                                                                                                               <  2:168438/62‑1 (MQ=255)                                                                                       actaTCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGa                                                                                                               >  1:168438/1‑62 (MQ=255)                                                                                       actaTCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTcaca                                                                         >  1:213829/1‑100 (MQ=255)                                                                                       actaTCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTcaca                                                                         <  2:213829/100‑1 (MQ=255)                                                                                       actaTCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATAc                                  >  1:557252/1‑139 (MQ=255)                                                                                           tCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTgg                              >  1:710538/1‑139 (MQ=255)                                                                                              ttCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAAC‑TTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTcc                          >  1:669138/1‑139 (MQ=255)                                                                                                ccACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGAt                                                                                                                   <  1:36072/49‑1 (MQ=255)                                                                                                ccACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGAt                                                                                                                   >  2:36072/1‑49 (MQ=255)                                                                                                ccACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACt                                        >  2:1296214/1‑124 (MQ=255)                                                                                                ccACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACt                                        <  1:1296214/124‑1 (MQ=255)                                                                                                   cTTTCCATGACAATAACGACAGCCTAATGCCAAAAt                                                                                                                             >  2:84189/1‑36 (MQ=255)                                                                                                   cTTTCCATGACAATAACGACAGCCTAATGCCAAAAt                                                                                                                             <  1:84189/36‑1 (MQ=255)                                                                                                   cTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAg                      >  1:926006/1‑139 (MQ=255)                                                                                                     ttCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTAc                                                                                                     <  2:597014/58‑1 (MQ=255)                                                                                                     ttCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTAc                                                                                                     >  1:597014/1‑58 (MQ=255)                                                                                                         aTGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTcc                >  1:42823/1‑139 (MQ=255)                                                                                                              aaTAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGgag           <  1:839260/139‑1 (MQ=255)                                                                                                                tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACtt                                                                                                        >  1:577892/1‑44 (MQ=255)                                                                                                                tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           <  1:619106/73‑1 (MQ=255)                                                                                                                tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           >  2:619106/1‑73 (MQ=255)                                                                                                                tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCgg              >  1:406690/1‑134 (MQ=255)                                                                                                                tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCgg              <  2:406690/134‑1 (MQ=255)                                                                                                                tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGAt         <  2:42823/139‑1 (MQ=255)                                                                                                                tAACGACAGCCTAATGCCAAAATTACGCCTGAACGTATTAACtt                                                                                                        <  2:577892/44‑1 (MQ=255)                                                                                                                  aCGACAGCCTAATGCCAAAATTACGCCTGATCGGATTACTTTAACTCGCATTTAGCGCAACTGCCGTCTCCCACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACTTGGGCCGCCACCGGTCCGGaagacaa       >  1:688133/1‑136 (MQ=255)                                                                                                                       aGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGATCATTATc  >  1:204396/1‑139 (MQ=255)                                                                                                                       aGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGATCATTATc  >  1:53615/1‑139 (MQ=255)                                                                                                                       aGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGATCATTATc  >  2:196189/1‑139 (MQ=255)                                                                                                                       aGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTGACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGATCATTATc  <  2:926006/139‑1 (MQ=255)                                                                                                                        gCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCggg             >  1:1313862/1‑127 (MQ=255)                                                                                                                        gCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCggg             <  2:1313862/127‑1 (MQ=255)                                                                                                                            |                                                                                                                                      GTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGC‑‑CACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTGATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGATCATTATC  >  NC_000913/3201085‑3201339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑