Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,232,174 C→T N170N (AAC→AAT)  fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,232,1740CT100.0% 152.5 / NA 48N170N (AAC→AAT) fadH2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/26);  total (22/26)

ACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGA  >  NC_000913/3232038‑3232309
                                                                                                                                        |                                                                                                                                       
aCCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGa                                                                                                                                       <  1:1043460/139‑1 (MQ=255)
               aTGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTgcg                                                                                                                        >  1:256623/1‑139 (MQ=255)
                       aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGACGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATc                                                                                                                <  2:939263/139‑1 (MQ=255)
                            tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGt                                                                                                           >  2:916596/1‑139 (MQ=255)
                                   aTCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGAcc                                                                                                    >  2:1114676/1‑139 (MQ=255)
                                       tGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACg                                                                                                                             <  1:632732/110‑1 (MQ=255)
                                       tGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACg                                                                                                                             >  2:632732/1‑110 (MQ=255)
                                       tGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCa                                                                                                                      >  1:726068/1‑117 (MQ=255)
                                       tGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCa                                                                                                                      <  2:726068/117‑1 (MQ=255)
                                         cAACTGATCGACAATTTCGCCCGCAGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTggg                                                                                              >  2:891744/1‑139 (MQ=255)
                                               aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCa                                                                                                                      >  1:882820/1‑109 (MQ=255)
                                               aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCa                                                                                                                      <  2:882820/109‑1 (MQ=255)
                                               aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGgcggcg                                                                                        >  2:1127650/1‑139 (MQ=255)
                                               aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGgcggcg                                                                                        >  2:102688/1‑139 (MQ=255)
                                                        ttCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCa                                                                               <  1:705797/139‑1 (MQ=255)
                                                         tCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCaa                                                                              >  1:266980/1‑139 (MQ=255)
                                                            cccGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCt                                                                                                                           <  2:617531/91‑1 (MQ=255)
                                                            cccGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCt                                                                                                                           >  1:617531/1‑91 (MQ=255)
                                                                cTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATg                                                                       >  2:303625/1‑139 (MQ=255)
                                                                cTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATg                                                                       >  1:1051089/1‑139 (MQ=255)
                                                                 tGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCt                                                                                                                           <  1:275460/86‑1 (MQ=255)
                                                                 tGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCt                                                                                                                           >  2:275460/1‑86 (MQ=255)
                                                                   cgcgcAACTGGCGCGGCAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCCATTACCGCAACCGGATCCGa                                                                    <  1:891744/139‑1 (MQ=255)
                                                                                 gggAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAagt                                                      <  1:303625/139‑1 (MQ=255)
                                                                                  ggAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAAt                                                                                                                 <  1:959355/79‑1 (MQ=255)
                                                                                  ggAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAAt                                                                                                                 >  2:959355/1‑79 (MQ=255)
                                                                                         ggATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                                              <  2:256623/139‑1 (MQ=255)
                                                                                                    tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAtt                                                                  >  1:1216410/1‑108 (MQ=255)
                                                                                                    tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAtt                                                                  <  2:1216410/108‑1 (MQ=255)
                                                                                                    tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGaa                                   <  1:916596/139‑1 (MQ=255)
                                                                                                    tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGaa                                   <  2:266980/139‑1 (MQ=255)
                                                                                                         agGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCa                                                                               >  2:396162/1‑90 (MQ=255)
                                                                                                         agGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCa                                                                               <  1:396162/90‑1 (MQ=255)
                                                                                                              aTGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCa                                                                               >  2:199412/1‑85 (MQ=255)
                                                                                                              aTGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCa                                                                               <  1:199412/85‑1 (MQ=255)
                                                                                                              aTGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGcg                         <  1:966/139‑2 (MQ=255)
                                                                                                               tGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        <  1:1114676/139‑1 (MQ=255)
                                                                                                                gggTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGTGCGAACGCGTCGGCAAc                       >  1:688761/1‑139 (MQ=255)
                                                                                                                gggTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAAc                       <  1:275686/139‑1 (MQ=255)
                                                                                                                      cGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGc                                                               <  1:842354/93‑1 (MQ=255)
                                                                                                                      cGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGc                                                               >  2:842354/1‑93 (MQ=255)
                                                                                                                       gAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCa                <  1:1085426/139‑1 (MQ=255)
                                                                                                                           ggTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCattat            <  2:1051089/139‑1 (MQ=255)
                                                                                                                           ggTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCattat            >  2:907141/1‑139 (MQ=255)
                                                                                                                                     cAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACtt                  <  1:1036839/123‑1 (MQ=255)
                                                                                                                                     cAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACtt                  >  2:1036839/1‑123 (MQ=255)
                                                                                                                                     cAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGa  <  1:1205880/139‑1 (MQ=255)
                                                                                                                                     cAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGa  <  1:102688/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
ACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGA  >  NC_000913/3232038‑3232309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑