Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,277,726	C→T	F130F (TTC→TTT)	yhaV →	toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,277,726	0	C	T	100.0%	236.6 / NA	72	F130F (TTC→TTT)	yhaV	toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/31);  total (41/31)

GGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTC  >  NC_000913/3277601‑3277861                                                                                                                              |                                                                                                                                        ggTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTaa                                                                                                                            >  1:1175011/1‑139 (MQ=255)     cTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAag                                                                                                                        <  2:372999/139‑1 (MQ=255)                   ctctTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTg                                                                                                          <  2:1175011/139‑1 (MQ=255)                    tcttctTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAag                                                                                                                        >  1:530241/1‑124 (MQ=255)                    tcttctTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAag                                                                                                                        <  2:530241/124‑1 (MQ=255)                      ttcttTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCg                                                                                                       >  1:224763/1‑139 (MQ=255)                       tcttTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGa                                                                                                      <  2:705067/139‑1 (MQ=255)                                ataGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAc                                                                                             >  1:610192/1‑139 (MQ=255)                                      aaaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAc                                                                                       >  1:130870/1‑139 (MQ=255)                                      aaaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAc                                                                                       >  2:1053367/1‑139 (MQ=255)                                       aaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                                                                                         >  1:1215529/1‑136 (MQ=255)                                       aaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                                                                                         <  2:1215529/136‑1 (MQ=255)                                         aaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGGAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCg                                                                                    <  2:1024674/139‑1 (MQ=255)                                             gaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGaa                                                                                <  2:311725/139‑1 (MQ=255)                                                aGTCATCATTCTGAGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACa                                                                             >  1:771127/1‑139 (MQ=255)                                                     tcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCa                                                                                                                                     <  1:1261057/78‑1 (MQ=255)                                                     tcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCa                                                                                                                                     >  2:1261057/1‑78 (MQ=255)                                                     tcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACagaaga                                                                        >  2:1114305/1‑139 (MQ=255)                                                     tcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACagaaga                                                                        >  1:447756/1‑139 (MQ=255)                                                      catTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGAc                                                                                                     <  1:468902/109‑1 (MQ=255)                                                      catTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGAc                                                                                                     >  2:468902/1‑109 (MQ=255)                                                            gggATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCaaa                                                                                                                                   <  1:242676/73‑1 (MQ=255)                                                            gggATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCaaa                                                                                                                                   >  2:242676/1‑73 (MQ=255)                                                                  gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATGCCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATgg                                                           >  1:887340/1‑139 (MQ=255)                                                                  gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAAc                                                                              >  1:396213/1‑120 (MQ=255)                                                                  gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAAc                                                                              <  2:396213/120‑1 (MQ=255)                                                                  gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATgg                                                           >  2:1232557/1‑139 (MQ=255)                                                                  gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATgg                                                           >  1:752278/1‑139 (MQ=255)                                                                  gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATgg                                                           >  1:1270794/1‑139 (MQ=255)                                                                  gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATgg                                                           >  2:35238/1‑139 (MQ=255)                                                                       aCGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTg                                                                                                          >  1:233112/1‑87 (MQ=255)                                                                       aCGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTg                                                                                                          <  2:233112/87‑1 (MQ=255)                                                                       aCGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa                                                                      >  2:440107/1‑123 (MQ=255)                                                                       aCGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa                                                                      <  1:346406/123‑1 (MQ=255)                                                                       aCGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa                                                                      <  1:440107/123‑1 (MQ=255)                                                                       aCGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa                                                                      >  2:346406/1‑123 (MQ=255)                                                                       aCGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTc                                                      >  1:525965/1‑139 (MQ=255)                                                                       aCGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCATCACCCGAGAAAc                                                                              >  1:1244450/1‑115 (MQ=255)                                                                       aCGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCATATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAAc                                                                              <  2:1244450/115‑1 (MQ=255)                                                                        cGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGa                                                                                                                    >  1:778087/1‑76 (MQ=255)                                                                        cGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGa                                                                                                                    <  2:778087/76‑1 (MQ=255)                                                                            gAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGTCTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAAt                                                 >  1:702613/1‑139 (MQ=255)                                                                                   ctctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCccgcc                                          <  2:887340/139‑1 (MQ=255)                                                                                     ctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATg                                                            <  1:563331/119‑1 (MQ=255)                                                                                     ctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATg                                                            >  2:563331/1‑119 (MQ=255)                                                                                     ctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGa                                        >  1:1308356/1‑139 (MQ=255)                                                                                     ctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCACCGa                                        >  1:823039/1‑139 (MQ=255)                                                                                      tGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGaa                                       <  1:416845/139‑1 (MQ=255)                                                                                      tGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGaa                                       <  2:130870/139‑1 (MQ=255)                                                                                        cgcATCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACg                                     >  1:371448/1‑139 (MQ=255)                                                                                          cACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATcctcct                                                                                                           >  1:584829/1‑67 (MQ=255)                                                                                          cACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATcctcct                                                                                                           <  2:584829/67‑1 (MQ=255)                                                                                          cACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCa                                   <  1:497613/139‑1 (MQ=255)                                                                                           aCCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAt                                  >  2:1249141/1‑139 (MQ=255)                                                                                           aCCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAt                                  <  1:620958/139‑1 (MQ=255)                                                                                            ccTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAtt                                 >  2:928924/1‑139 (MQ=255)                                                                                             cTACGGTAAAGAACCAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTc                                >  2:880859/1‑139 (MQ=255)                                                                                                     aaaaaaCAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAAc                                                                              >  1:1051050/1‑85 (MQ=255)                                                                                                     aaaaaaCAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAAc                                                                              <  2:1051050/85‑1 (MQ=255)                                                                                                           cAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                                                                                                 >  1:715728/1‑60 (MQ=255)                                                                                                           cAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                                                                                                 <  2:715728/60‑1 (MQ=255)                                                                                                             gATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGc                >  1:1301264/1‑139 (MQ=255)                                                                                                              aTGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGAACAGCGGGCa               <  1:880859/139‑1 (MQ=255)                                                                                                                     taCCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGa                                        <  2:552543/107‑1 (MQ=255)                                                                                                                     taCCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGa                                        >  1:552543/1‑107 (MQ=255)                                                                                                                       ccGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTtctc      <  2:284364/139‑1 (MQ=255)                                                                                                                       ccGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTtctc      <  2:771127/139‑1 (MQ=255)                                                                                                                          tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                                                                                         <  1:170841/53‑1 (MQ=255)                                                                                                                          tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                                                                                         >  2:170841/1‑53 (MQ=255)                                                                                                                          tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCa               >  1:1180981/1‑127 (MQ=255)                                                                                                                          tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCa               <  2:1180981/127‑1 (MQ=255)                                                                                                                           aTTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCCCAATGCCCGCCGAACGCATTTGTGTTGACCAGCGGGCATCCTTTCTTACTc  >  1:938711/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                        GGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTC  >  NC_000913/3277601‑3277861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑