Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,328,103 A→C intergenic (+20/+136) yhbY → / ← greA RNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,328,1030AC100.0% 106.7 / NA 30intergenic (+20/+136)yhbY/greARNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (13/17);  total (13/17)

CCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTA  >  NC_000913/3327968‑3328234
                                                                                                                                        |                                                                                                                                   
ccTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCAt                                                                                                                                  <  1:1211907/139‑1 (MQ=255)
ccTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCAt                                                                                                                                  <  1:887292/139‑1 (MQ=255)
                       cgcgAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGATAAGATTAACCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGttt                                                                                                           >  2:359159/1‑139 (MQ=255)
                             aCCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCgct                                                                                                     <  1:772453/139‑2 (MQ=255)
                                 gcgcCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCCATTTGCTGTGTAAAACGAGGGGTTTTCCgcaggc                                                                                                  <  2:1161294/139‑1 (MQ=255)
                                                 ggTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCa                                                                                 <  2:959007/139‑1 (MQ=255)
                                                  gTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTg                                                                                                                               <  1:285358/92‑1 (MQ=255)
                                                  gTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTg                                                                                                                               >  2:285358/1‑92 (MQ=255)
                                                  gTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCAc                                                                                >  2:724157/1‑139 (MQ=255)
                                                  gTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCAc                                                                                >  2:1157721/1‑139 (MQ=255)
                                                       cGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGctct                                                                           >  2:978145/1‑139 (MQ=255)
                                                           aaaaCGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTc                                                                       <  2:998972/139‑1 (MQ=255)
                                                             aaCGCTGGTGCTTTATCGCGCAACTAAAGAACGTAAAATCGCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCAGTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGt                                                                     <  2:1278200/139‑1 (MQ=255)
                                                                 cTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGAt                                                                 >  2:131520/1‑139 (MQ=255)
                                                                               cccAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAg                                                   >  2:61203/1‑139 (MQ=255)
                                                                                 cAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAGAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCa                                                                                         <  2:201618/99‑1 (MQ=255)
                                                                                 cAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAGAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCa                                                                                         >  1:201618/1‑99 (MQ=255)
                                                                                       aaGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCttt                                           <  1:1157721/139‑1 (MQ=255)
                                                                                            cGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCct                                      <  1:978145/139‑1 (MQ=255)
                                                                                            cGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCct                                      <  1:61203/139‑1 (MQ=255)
                                                                                              tAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCcttt                                    >  2:306289/1‑139 (MQ=255)
                                                                                               aaaaTCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCctttc                                   >  1:318393/1‑139 (MQ=255)
                                                                                                      cGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACaa                            <  1:306289/139‑1 (MQ=255)
                                                                                                         tGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATAc                         >  1:73966/1‑139 (MQ=255)
                                                                                                                    gATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCtt              <  2:318393/139‑1 (MQ=255)
                                                                                                                         tCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTa         >  2:1165230/1‑139 (MQ=255)
                                                                                                                         tCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTa         <  1:724157/139‑1 (MQ=255)
                                                                                                                         tCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTa         <  1:1165230/139‑1 (MQ=255)
                                                                                                                               aGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGt   >  1:1215276/1‑139 (MQ=255)
                                                                                                                                gTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTa  <  2:1215276/139‑1 (MQ=255)
                                                                                                                                   acacccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGctc                                                                            <  2:571478/62‑1 (MQ=255)
                                                                                                                                   acacccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGctc                                                                            >  1:571478/1‑62 (MQ=255)
                                                                                                                                   acacccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCttttt                                         >  2:428649/1‑97 (MQ=255)
                                                                                                                                   acacccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCttttt                                         <  1:428649/97‑1 (MQ=255)
                                                                                                                                   acacccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCct                                      <  1:662744/100‑1 (MQ=255)
                                                                                                                                   acacccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCct                                      >  2:47685/1‑100 (MQ=255)
                                                                                                                                   acacccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCct                                      >  2:662744/1‑100 (MQ=255)
                                                                                                                                   acacccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCct                                      <  1:47685/100‑1 (MQ=255)
                                                                                                                                   acacccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACaa                            <  1:971980/110‑1 (MQ=255)
                                                                                                                                   acacccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACaa                            >  2:971980/1‑110 (MQ=255)
                                                                                                                                    cacccaTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGcc                                              <  2:249876/91‑1 (MQ=255)
                                                                                                                                    cacccaTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGt   <  2:566865/134‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                   
CCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTA  >  NC_000913/3327968‑3328234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: