| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,353,155 | G→A | A299V (GCG→GTG) | yhcC ← | putative Fe‑S oxidoreductase, Radical SAM superfamily protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,353,155 | 0 | G | A | 97.8% | 142.1 / ‑4.9 | 46 | A299V (GCG→GTG) | yhcC | putative Fe‑S oxidoreductase, Radical SAM superfamily protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (22/23); total (22/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACCTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACT > NC_000913/3353022‑3353286 | tCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACCTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGAt < 1:531106/139‑1 (MQ=255) ttAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCAGTTGGAGGTAGCCACGGACGTCCCAGCACCGATccc > 1:824515/1‑139 (MQ=255) ggAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTa > 2:918176/1‑139 (MQ=255) ttCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGcc > 2:329784/1‑139 (MQ=255) ttCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGcc < 2:1171037/139‑1 (MQ=255) ttCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGcc > 2:653564/1‑139 (MQ=255) tCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCa < 1:1297434/139‑1 (MQ=255) cTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGa < 1:31065/124‑1 (MQ=255) cTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGa > 2:31065/1‑124 (MQ=255) tCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGTAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGtt < 2:928217/139‑1 (MQ=255) tCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGtt < 1:653564/139‑1 (MQ=255) cGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGttcat > 2:103724/1‑139 (MQ=255) aataCCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGc < 2:1004302/139‑1 (MQ=255) aCCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGGGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGc < 1:619911/110‑1 (MQ=255) aCCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGGGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGc > 2:619911/1‑110 (MQ=255) aCCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGttcat < 2:139806/119‑1 (MQ=255) aCCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGttcat > 1:139806/1‑119 (MQ=255) aCCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCg > 2:651284/1‑139 (MQ=255) aCCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCg > 2:699311/1‑139 (MQ=255) ccAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGAc < 1:918176/139‑1 (MQ=255) aaTTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCa > 2:786971/1‑139 (MQ=255) tACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATc > 2:724055/1‑139 (MQ=255) taAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGttcatt > 1:1251043/1‑97 (MQ=255) taAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGttcatt < 2:1251043/97‑1 (MQ=255) aaaTTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCg < 2:159658/139‑1 (MQ=255) aaTTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGc > 2:1116119/1‑139 (MQ=255) gtgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcacca < 1:103724/139‑1 (MQ=255) tgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAg < 2:279656/97‑1 (MQ=255) tgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAg > 1:279656/1‑97 (MQ=255) gaAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCgg < 1:651284/139‑1 (MQ=255) aaTTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGc > 2:382945/1‑128 (MQ=255) aaTTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGc < 1:382945/128‑1 (MQ=255) aCTCCGTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCt > 1:341696/1‑139 (MQ=255) gTTGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCgg > 2:557033/1‑139 (MQ=255) ttGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGa < 2:52400/139‑1 (MQ=255) tGGAGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGaa > 1:926566/1‑139 (MQ=255) aGGTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATg < 1:1116119/139‑1 (MQ=255) ggTAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGc < 2:341696/139‑1 (MQ=255) tAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCgg < 1:699311/139‑1 (MQ=255) tAGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCgg < 1:329784/139‑1 (MQ=255) aGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGa > 1:45888/1‑64 (MQ=255) aGCCACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGa < 2:45888/64‑1 (MQ=255) ccACGGACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCgctgg < 1:894563/139‑1 (MQ=255) gACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGTCGACGGGCGCTGGCGGAAATGCGGTGGTAGAt < 2:781631/139‑1 (MQ=255) gACGTCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGAt > 1:974285/1‑139 (MQ=255) tCCCAGCACCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACt > 2:908119/1‑139 (MQ=255) | TCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACCTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACT > NC_000913/3353022‑3353286 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |