Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,440,950 G→A T27I (ACC→ATC)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,440,9500GA100.0% 298.7 / NA 92T27I (ACC→ATC) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (50/42);  total (50/42)

TTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGT  >  NC_000913/3440814‑3441083
                                                                                                                                        |                                                                                                                                     
ttcttTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATc                                                                                                                                     >  1:109867/1‑139 (MQ=255)
        tGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGgc                                                                                                                             >  1:1199617/1‑139 (MQ=255)
         gCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGgcg                                                                                                                            <  2:619224/139‑1 (MQ=255)
             tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCAc                                                                                                                                   >  1:629038/1‑128 (MQ=255)
             tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCAc                                                                                                                                   <  2:629038/128‑1 (MQ=255)
             tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGgcgtgcg                                                                                                                        >  1:678954/1‑139 (MQ=255)
                 cATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGa                                                                                                                    >  1:460920/1‑139 (MQ=255)
                  aTGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGaa                                                                                                                   >  2:532370/1‑139 (MQ=255)
                  aTGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGaa                                                                                                                   >  2:1310941/1‑139 (MQ=255)
                       gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc                                                                                                              <  1:532370/139‑1 (MQ=255)
                       gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc                                                                                                              >  1:16791/1‑139 (MQ=255)
                       gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc                                                                                                              >  2:731976/1‑139 (MQ=255)
                       gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc                                                                                                              >  2:1004437/1‑139 (MQ=255)
                        taCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACt                                                                                                             <  2:460920/139‑1 (MQ=255)
                          cacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTg                                                                                                           >  2:364446/1‑139 (MQ=255)
                            ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                         >  2:66194/1‑139 (MQ=255)
                            ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                         <  2:678027/139‑1 (MQ=255)
                            ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                         <  1:356967/139‑1 (MQ=255)
                            ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                         >  2:1286254/1‑139 (MQ=255)
                             cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                                        >  2:1176193/1‑139 (MQ=255)
                              aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACACACTTGCTCg                                                                                                       >  2:1291876/1‑139 (MQ=255)
                               tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGa                                                                                                      >  1:33743/1‑139 (MQ=255)
                               tCAATCTCAACCTCGGTCACAGCGCCACCGGACGTCGATGAGAGAAGAATACGAATCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTTTCACGGCTCAAGGATCACCTTGGCGTCAGTCGAACTCACTTGGTCGa                                                                                                      >  2:949205/1‑139 (MQ=255)
                                  aTCTCAACCTCGGTCACCGCGCAACCCGGGATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATTACTTTGGCGTGCGTCGAACTCACTTGATCGatat                                                                                                   >  2:1261369/1‑139 (MQ=255)
                                  aTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGatat                                                                                                   >  1:136017/1‑139 (MQ=255)
                                  aTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGatat                                                                                                   >  1:548964/1‑139 (MQ=255)
                                  aTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGatat                                                                                                   >  2:903210/1‑139 (MQ=255)
                                  aTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGatat                                                                                                   >  1:640833/1‑139 (MQ=255)
                                         ccTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACtt                                                                                                            <  2:639472/123‑1 (MQ=255)
                                         ccTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACtt                                                                                                            >  1:639472/1‑123 (MQ=255)
                                            cGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                                         <  1:916030/139‑1 (MQ=255)
                                            cGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                                         <  2:109867/139‑1 (MQ=255)
                                                   cgcgCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGtttt                                                                                  >  2:952294/1‑139 (MQ=255)
                                                        aaCCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGaaa                                                                             <  1:405803/139‑1 (MQ=255)
                                                             ggCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTg                                                                        <  1:1113465/139‑1 (MQ=255)
                                                                    aTGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGa                                                                 <  1:1176193/139‑1 (MQ=255)
                                                                    aTGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAC‑TGCTCGATATCAACCAGGCGCGGTTTTAGAAActc                                                                          <  2:1095452/129‑1 (MQ=255)
                                                                    aTGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAC‑TGCTCGATATCAACCAGGCGCGGTTTTAGAAActc                                                                          >  1:1095452/1‑129 (MQ=255)
                                                                             gAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCAt                                                        <  1:903210/139‑1 (MQ=255)
                                                                              aaTACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAAGCGCGGTTTTAGAAActc                                                                          >  1:883490/1‑120 (MQ=255)
                                                                              aaTACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAAGCGCGGTTTTAGAAActc                                                                          <  2:883490/120‑1 (MQ=255)
                                                                                 actgcgcAGTGCGTTTCGCAGAGAATGTCAAATGCGTCTCTGTAAAGGCTCAAAGATCGCCTTGGCTTGGGTCGAACTCTCTTGCTCGATATCAACCAGGCACGGTTTTAGAAACTCTTTCTCAGAACCCTGCATtgtg                                                    <  2:33743/136‑1 (MQ=255)
                                                                                   ggCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTc                                                  <  2:548964/139‑1 (MQ=255)
                                                                                        aGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                         >  2:160873/1‑79 (MQ=255)
                                                                                        aGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                         <  1:160873/79‑1 (MQ=255)
                                                                                        aGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATtgt                                                     >  1:165277/1‑131 (MQ=255)
                                                                                        aGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATtgt                                                     <  2:165277/131‑1 (MQ=255)
                                                                                          tGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                           <  1:1158379/139‑1 (MQ=255)
                                                                                          tGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                           <  1:952294/139‑1 (MQ=255)
                                                                                          tGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                           >  2:1158379/1‑139 (MQ=255)
                                                                                                    agagTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGaa                                                                                                                   <  1:554372/57‑1 (MQ=255)
                                                                                                    agagTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGaa                                                                                                                   >  2:554372/1‑57 (MQ=255)
                                                                                                    agagTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                           <  2:1210740/129‑1 (MQ=255)
                                                                                                    agagTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                           >  1:1210740/1‑129 (MQ=255)
                                                                                                    agagTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAg                                 >  2:1277373/1‑139 (MQ=255)
                                                                                                    agagTATGGACAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                                   <  2:853063/105‑1 (MQ=255)
                                                                                                    agagTATGCCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                                   >  1:853063/1‑105 (MQ=255)
                                                                                                      agTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                         >  1:540629/1‑65 (MQ=255)
                                                                                                      agTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                         <  2:540629/65‑1 (MQ=255)
                                                                                                      agTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                                         <  2:1011569/81‑1 (MQ=255)
                                                                                                      agTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                                         >  1:1011569/1‑81 (MQ=255)
                                                                                                      agTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                           >  1:487874/1‑127 (MQ=255)
                                                                                                      agTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                           <  2:487874/127‑1 (MQ=255)
                                                                                                           ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt                          >  2:1207451/1‑139 (MQ=255)
                                                                                                           ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt                          >  2:834746/1‑139 (MQ=255)
                                                                                                           ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt                          >  2:383180/1‑139 (MQ=255)
                                                                                                                aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTc                                                                                                                      <  2:964324/42‑1 (MQ=255)
                                                                                                                aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTc                                                                                                                      >  1:964324/1‑42 (MQ=255)
                                                                                                                aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc                                                                                                              <  1:1110811/50‑1 (MQ=255)
                                                                                                                aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc                                                                                                              >  2:1110811/1‑50 (MQ=255)
                                                                                                                aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                                         <  1:569138/71‑1 (MQ=255)
                                                                                                                aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                                         >  2:569138/1‑71 (MQ=255)
                                                                                                                 aGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTGACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGt                    >  1:832608/1‑139 (MQ=255)
                                                                                                                 aGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGt                    >  1:873182/1‑139 (MQ=255)
                                                                                                                    cACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTaaa                 <  1:834746/139‑1 (MQ=255)
                                                                                                                      cGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcgc                                                                                        >  1:1110384/1‑66 (MQ=255)
                                                                                                                      cGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcgc                                                                                        <  2:1110384/66‑1 (MQ=255)
                                                                                                                      cGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGaaa                                                                             >  2:96146/1‑77 (MQ=255)
                                                                                                                      cGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGaaa                                                                             <  1:96146/77‑1 (MQ=255)
                                                                                                                         tctAAAGGCTCAAGGATCACCTTGTCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTcg            <  2:640833/139‑1 (MQ=255)
                                                                                                                             aaGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTcgacga        <  1:416700/139‑1 (MQ=255)
                                                                                                                                 cTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGc                                                          <  1:1291303/85‑1 (MQ=255)
                                                                                                                                 cTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGc                                                          <  1:1299631/85‑1 (MQ=255)
                                                                                                                                 cTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGc                                                          >  2:1291303/1‑85 (MQ=255)
                                                                                                                                 cTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGc                                                          >  2:1299631/1‑85 (MQ=255)
                                                                                                                                 cTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAg    <  2:1199617/139‑1 (MQ=255)
                                                                                                                                   cAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                                   <  2:752998/74‑1 (MQ=255)
                                                                                                                                   cAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                                   >  1:752998/1‑74 (MQ=255)
                                                                                                                                   cAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                           <  1:467419/98‑1 (MQ=255)
                                                                                                                                   cAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                           >  2:467419/1‑98 (MQ=255)
                                                                                                                                   cAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGAt       >  2:443244/1‑134 (MQ=255)
                                                                                                                                   cAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGAt       <  1:443244/134‑1 (MQ=255)
                                                                                                                                   cAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGgt  <  2:873182/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                     
TTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGT  >  NC_000913/3440814‑3441083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑