Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	335,970	C→T	Q16* (CAA→TAA)	yahE →	DUF2877 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	335,970	0	C	T	100.0%	188.8 / NA	58	Q16* (CAA→TAA)	yahE	DUF2877 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/31);  total (27/31)

AACGGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTTG  >  NC_000913/335836‑336102                                                                                                                                       |                                                                                                                                     aaCGGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAgg                                                                                                                                  <  1:1029104/139‑1 (MQ=255)      ggCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAgg                                                                                                                             <  1:206009/139‑1 (MQ=255)                          gTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCaga                                                                                                         <  2:131012/139‑1 (MQ=255)                           ttACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAAc                                                                                                                       <  2:299532/124‑1 (MQ=255)                           ttACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAAc                                                                                                                       >  1:299532/1‑124 (MQ=255)                                 aTTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCaga                                                                                                         <  1:940459/132‑1 (MQ=255)                                 aTTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCaga                                                                                                         >  2:940459/1‑132 (MQ=255)                                   tGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAat                                                                                                >  2:148106/1‑139 (MQ=255)                                     ccGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                           <  2:578394/126‑1 (MQ=255)                                     ccGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                           >  1:578394/1‑126 (MQ=255)                                     ccGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAatat                                                                                              >  1:156707/1‑139 (MQ=255)                                     ccGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAatat                                                                                              >  2:631216/1‑139 (MQ=255)                                     ccGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAatat                                                                                              >  1:482189/1‑139 (MQ=255)                                       gCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCg                                                                                            <  2:482189/139‑1 (MQ=255)                                               aTAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCg                                                                                    >  1:1186351/1‑139 (MQ=255)                                                     cGGGAGGCTTGCTATCAACACACCAGATAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                           >  2:476776/1‑110 (MQ=255)                                                     cGGGAGGCTTGCTATCAACACACCAGATAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                           <  1:476776/110‑1 (MQ=255)                                                     cGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGAGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGc                                                                              >  2:524449/1‑139 (MQ=255)                                                        gAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCg                                                                                    <  1:408968/130‑1 (MQ=255)                                                        gAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCg                                                                                    >  2:408968/1‑130 (MQ=255)                                                        gAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCcag                                                                           <  2:89522/139‑1 (MQ=255)                                                           gCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAgg                                                                                                                                  <  2:188924/81‑1 (MQ=255)                                                           gCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAgg                                                                                                                                  >  1:188924/1‑81 (MQ=255)                                                                  aTCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCg                                                                               <  1:857658/125‑1 (MQ=255)                                                                  aTCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCg                                                                               >  2:857658/1‑125 (MQ=255)                                                                    cAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACg                                                               <  2:1186351/139‑1 (MQ=255)                                                                    cAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCGTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACg                                                               <  1:148106/139‑1 (MQ=255)                                                                    cAACACACCAGAAAGACGGTGTGTGTGAGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACg                                                               <  1:524449/139‑1 (MQ=255)                                                                          acCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAgg                                                                                                                                  <  1:500723/66‑1 (MQ=255)                                                                          acCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAgg                                                                                                                                  >  2:500723/1‑66 (MQ=255)                                                                              gAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                                     <  1:219101/139‑1 (MQ=255)                                                                              gAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATATTCTGGCGCAGCGGGGGTAAGGACAGCTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCAGACGCTGCTTtgtg                                                     <  1:798559/139‑1 (MQ=255)                                                                               aaaGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCaga                                                                                                         >  2:577905/1‑86 (MQ=255)                                                                               aaaGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCaga                                                                                                         <  1:577905/86‑1 (MQ=255)                                                                               aaaGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgctgct                                                          <  1:1264086/133‑1 (MQ=255)                                                                               aaaGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgctgct                                                          >  2:1264086/1‑133 (MQ=255)                                                                                aaGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGaa                                                   <  2:992378/139‑1 (MQ=255)                                                                                      gtgtgtgtGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTAc                                             >  1:940069/1‑139 (MQ=255)                                                                                      gtgtgtgtGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTAc                                             >  2:153558/1‑139 (MQ=255)                                                                                        gtgtgtgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                           <  2:330725/75‑1 (MQ=255)                                                                                        gtgtgtgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                           >  1:330725/1‑75 (MQ=255)                                                                                         tgtgtgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgctgc                                                           >  1:611829/1‑122 (MQ=255)                                                                                         tgtgtgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgctgc                                                           <  2:611829/122‑1 (MQ=255)                                                                                           tgtgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGt                                                                       <  1:591948/108‑1 (MQ=255)                                                                                           tgtgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGt                                                                       >  2:591948/1‑108 (MQ=255)                                                                                              gggCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCcag                                                                           <  2:469614/101‑1 (MQ=255)                                                                                              gggCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCcag                                                                           >  1:469614/1‑101 (MQ=255)                                                                                                 cgcTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGcc                                  >  1:1101871/1‑139 (MQ=255)                                                                                                   cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCaa                                >  1:999578/1‑139 (MQ=255)                                                                                                    tAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCaaa                               <  2:878317/139‑1 (MQ=255)                                                                                                                    ttttCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGca               <  2:999578/139‑1 (MQ=255)                                                                                                                        cTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactca           <  2:1103085/139‑1 (MQ=255)                                                                                                                        cTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactca           <  1:385477/139‑1 (MQ=255)                                                                                                                            cgcAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcactca       <  2:940069/139‑1 (MQ=255)                                                                                                                             gcAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcactcac      >  2:1104815/1‑139 (MQ=255)                                                                                                                                 cGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTtg  >  2:864985/1‑139 (MQ=255)                                                                                                                                 cGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTtg  <  1:752772/139‑1 (MQ=255)                                                                                                                                 cGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTtg  >  1:457327/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                     AACGGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTTG  >  NC_000913/335836‑336102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑