Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,694,840	G→A	H133Y (CAT→TAT)	bcsA ←	cellulose synthase, catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,694,840	0	G	A	100.0%	192.0 / NA	59	H133Y (CAT→TAT)	bcsA	cellulose synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/29);  total (30/29)

TAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATGCTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCACCGATCAATA  >  NC_000913/3694705‑3694971                                                                                                                                        |                                                                                                                                    tAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACtg                                                                                                                                  <  2:998776/139‑1 (MQ=255) tAAACTGCGCCAGCGGGTTAAACGGCCGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAAACGCAGCGCCCGCGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACtg                                                                                                                                  >  2:67382/1‑139 (MQ=255)       gcgcCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGtttt                                                                                                                            >  1:1086651/1‑139 (MQ=255)       gcgcCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGtttt                                                                                                                            >  2:312307/1‑139 (MQ=255)        cgcCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTa                                                                                                                           >  2:304043/1‑139 (MQ=255)          ccAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATg                                                                                                                         >  1:576272/1‑139 (MQ=255)               gggTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    <  2:408659/139‑1 (MQ=255)               gggTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    <  1:312307/139‑1 (MQ=255)               gggTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    <  2:1151347/139‑1 (MQ=255)                  ttAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAAcgc                                                                                                                 >  2:445192/1‑139 (MQ=255)                          cTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGc                                                                                                         >  1:634257/1‑139 (MQ=255)                           tGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCa                                                                                                        >  2:1263085/1‑139 (MQ=255)                           tGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCa                                                                                                        <  1:997107/139‑1 (MQ=255)                            gAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAg                                                                                                       <  2:404656/139‑1 (MQ=255)                              gTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCt                                                                                                     <  1:1263085/139‑1 (MQ=255)                                    cAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCa                                                                                               >  2:316483/1‑139 (MQ=255)                                    cAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCa                                                                                               >  2:1120937/1‑139 (MQ=255)                                      gATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATc                                                                                             <  1:1120937/139‑1 (MQ=255)                                       aTTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATcc                                                                                            >  1:84123/1‑139 (MQ=255)                                            cGCAAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATAATGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGa                                                                                       >  2:847197/1‑139 (MQ=255)                                                 gAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGttt                                                                                                                             >  1:829320/1‑96 (MQ=255)                                                 gAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGttt                                                                                                                             <  2:829320/96‑1 (MQ=255)                                                         gagaAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCAc                                                                          <  1:903734/139‑1 (MQ=255)                                                         gagaAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCAc                                                                          <  1:304043/139‑1 (MQ=255)                                                         gagaAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCAc                                                                          >  2:573980/1‑139 (MQ=255)                                                            aaGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGaa                                                                       >  1:845242/1‑139 (MQ=255)                                                                  aCGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    <  2:994343/88‑1 (MQ=255)                                                                  aCGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    >  1:994343/1‑88 (MQ=255)                                                                    gatgatACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCGAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGc                                                               <  2:845242/139‑1 (MQ=255)                                                                        atACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGAc                                                           <  1:316483/139‑1 (MQ=255)                                                                         tACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTa                                                                                                                           >  2:276383/1‑74 (MQ=255)                                                                         tACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTa                                                                                                                           <  1:276383/74‑1 (MQ=255)                                                                         tACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTcc                                                                            >  1:251140/1‑121 (MQ=255)                                                                         tACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTcc                                                                            <  2:251140/121‑1 (MQ=255)                                                                              aGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCAt                                                                                              >  2:705049/1‑98 (MQ=255)                                                                              aGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCAt                                                                                              <  1:705049/98‑1 (MQ=255)                                                                              aGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGaaa                                                     >  1:619099/1‑139 (MQ=255)                                                                                     aCCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAg                                                                                                       <  1:1191896/82‑1 (MQ=255)                                                                                     aCCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAg                                                                                                       >  2:1191896/1‑82 (MQ=255)                                                                                        gccgcGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGtt                                                                                                 <  2:413140/85‑1 (MQ=255)                                                                                        gccgcGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGtt                                                                                                 >  1:413140/1‑85 (MQ=255)                                                                                                      cTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCg                             <  2:634257/139‑1 (MQ=255)                                                                                                         aaaTGACCGAGCTCTGTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGttt                          >  2:879201/1‑139 (MQ=255)                                                                                                           aTGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCGGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCt                        >  1:251900/1‑139 (MQ=255)                                                                                                              aCCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTg                     >  2:703350/1‑139 (MQ=255)                                                                                                                cGAACTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTgcg                   <  1:847197/139‑1 (MQ=255)                                                                                                                  aGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGcgcg                 <  1:703350/139‑1 (MQ=255)                                                                                                                  aGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATA‑TTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGa                <  2:1086651/139‑1 (MQ=255)                                                                                                                   gCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTgcg                                        >  1:290574/1‑115 (MQ=255)                                                                                                                   gCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTgcg                                        <  2:290574/115‑1 (MQ=255)                                                                                                                       ttttttCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGTAAATGTCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCa            <  1:879201/139‑1 (MQ=255)                                                                                                                         ttttCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCAcc          <  2:619099/139‑1 (MQ=255)                                                                                                                         ttttCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCAcc          <  2:1132077/139‑1 (MQ=255)                                                                                                                             ctcATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCACCGATc      >  1:354467/1‑139 (MQ=255)                                                                                                                                 tCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGc                                                               >  2:735744/1‑78 (MQ=255)                                                                                                                                 tCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGc                                                               <  1:735744/78‑1 (MQ=255)                                                                                                                                 tCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTgcg                   >  1:1256587/1‑122 (MQ=255)                                                                                                                                 tCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTgcg                   <  2:1256587/122‑1 (MQ=255)                                                                                                                                 tCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCACCGATCAATa  >  1:627411/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                    TAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATGCTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCACCGATCAATA  >  NC_000913/3694705‑3694971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑