Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,789,047	C→T	A316V (GCT→GTT)  *345* (TAG→TAA)	yibQ → waaH ←	putative polysaccharide deacetylase LPS(HepIII)‑glucuronic acid glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,789,047	0	C	T	100.0%	197.0 / NA	60	A316V (GCT→GTT)  *345* (TAG→TAA)	yibQ waaH	putative polysaccharide deacetylase LPS(HepIII)‑glucuronic acid glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/29);  total (31/29)

TGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTA  >  NC_000913/3788931‑3789182                                                                                                                     |                                                                                                                                        tGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTAc                                                                                                                   >  1:740972/1‑139 (MQ=255)  gCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTAcg                                                                                                                  <  2:981087/139‑1 (MQ=255)  gCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTAcg                                                                                                                  >  1:319241/1‑139 (MQ=255)   cTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTAcgc                                                                                                                 >  2:603693/1‑139 (MQ=255)       tCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTaa                                                                                                             >  2:165234/1‑139 (MQ=255)       tCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTaa                                                                                                             >  2:417441/1‑139 (MQ=255)          cttcttTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGc                                                                                                          <  1:616143/139‑1 (MQ=255)                    gTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGa                                                                                                <  2:319241/139‑1 (MQ=255)                       ttAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACcg                                                                                             >  2:1097516/1‑139 (MQ=255)                       ttAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACcg                                                                                             >  1:630325/1‑139 (MQ=255)                         aaGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACcgcg                                                                                           <  1:165234/139‑1 (MQ=255)                             gAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGtttt                                                                                       >  2:355064/1‑139 (MQ=255)                               aaGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTAcgcg                                                                                                                >  2:415547/1‑112 (MQ=255)                               aaGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTAcgcg                                                                                                                <  1:415547/112‑1 (MQ=255)                                   aTCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTcgcg                                                                                 <  1:417441/139‑1 (MQ=255)                                   aTCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCATTTTGTcgcg                                                                                 <  2:740972/139‑1 (MQ=255)                                            agCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCa                                                                        >  1:1254054/1‑139 (MQ=255)                                               aCGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATaa                                                                     <  2:834223/139‑1 (MQ=255)                                                    gATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGa                                                                <  1:355064/139‑1 (MQ=255)                                                      tCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGTGATGGTTCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGaaa                                                              <  2:929886/139‑1 (MQ=255)                                                      tCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTTCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGaaa                                                              >  1:929886/1‑139 (MQ=255)                                                      tCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCgcgc                                                                                >  1:741085/1‑121 (MQ=255)                                                      tCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCgcgc                                                                                <  2:741085/121‑1 (MQ=255)                                                           tACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACca                                                         >  1:1034437/1‑139 (MQ=255)                                                               tCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATa                                                                      <  2:1050782/122‑1 (MQ=255)                                                               tCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATa                                                                      >  1:1050782/1‑122 (MQ=255)                                                               tCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGt                                                     >  1:1265017/1‑139 (MQ=255)                                                                  aGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCAATTGCCATAAGCGGAAAGACCACAGTAAc                                                  >  2:563179/1‑139 (MQ=255)                                                                   gCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGAc                                                           >  1:1129878/1‑129 (MQ=255)                                                                   gCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGAc                                                           <  2:1129878/129‑1 (MQ=255)                                                                        aGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAg                                                                                                                                      <  2:861043/49‑1 (MQ=255)                                                                        aGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAg                                                                                                                                      >  1:861043/1‑49 (MQ=255)                                                                        aGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTgg                                            >  2:698311/1‑139 (MQ=255)                                                                          tGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTa                                          >  1:1058346/1‑139 (MQ=255)                                                                           ggCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCAATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTaa                                         <  1:563179/139‑1 (MQ=255)                                                                                 gCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCa                                                                              <  2:1048287/96‑1 (MQ=255)                                                                                 gCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCa                                                                              >  1:1048287/1‑96 (MQ=255)                                                                                  cTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTcgcg                                                                                 >  2:308427/1‑92 (MQ=255)                                                                                  cTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTcgcg                                                                                 <  1:308427/92‑1 (MQ=255)                                                                                  cTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCt                                  <  2:630325/139‑1 (MQ=255)                                                                                    ggggCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGgcg                                                                                                                       <  2:1064654/52‑1 (MQ=255)                                                                                    ggggCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGgcg                                                                                                                       >  1:1064654/1‑52 (MQ=255)                                                                                    ggggCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCa                                                                              >  2:8322/1‑93 (MQ=255)                                                                                    ggggCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCa                                                                              <  1:8322/93‑1 (MQ=255)                                                                                    ggggCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTc                                >  2:871504/1‑139 (MQ=255)                                                                                          aaCAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTg                          <  1:603693/139‑1 (MQ=255)                                                                                           aCAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTcg                                                                                   <  1:1169359/81‑1 (MQ=255)                                                                                           aCAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTcg                                                                                   >  2:1169359/1‑81 (MQ=255)                                                                                           aCAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAAcc                                       >  2:280395/1‑125 (MQ=255)                                                                                           aCAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAAcc                                       <  1:280395/125‑1 (MQ=255)                                                                                              gCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGca                      <  2:1254054/139‑1 (MQ=255)                                                                                               cccGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGAcc                                                          >  1:489700/1‑102 (MQ=255)                                                                                               cccGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGAcc                                                          <  2:489700/102‑1 (MQ=255)                                                                                                ccGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGcaca                    >  1:1111249/1‑139 (MQ=255)                                                                                                         cgAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATc           >  2:1205595/1‑139 (MQ=255)                                                                                                          gAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCa          <  1:1097516/139‑1 (MQ=255)                                                                                                          gAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCa          <  2:1265017/139‑1 (MQ=255)                                                                                                              tttAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATa                                                                      >  2:1051263/1‑75 (MQ=255)                                                                                                              tttAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATa                                                                      <  1:1051263/75‑1 (MQ=255)                                                                                                                  aCGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTta  <  1:843664/139‑1 (MQ=255)                                                                                                                     |                                                                                                                                        TGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTA  >  NC_000913/3788931‑3789182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑