Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	354,592	G→A	*629* (TAG→TAA)	prpE →	propionate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,592	0	G	A	100.0%	221.4 / NA	68	*629* (TAG→TAA)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/30);  total (38/30)

GATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATG  >  NC_000913/354471‑354726                                                                                                                           |                                                                                                                                       gATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTAtt                                                                                                                                  >  2:1180887/1‑128 (MQ=255) gATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTAtt                                                                                                                                  <  1:1180887/128‑1 (MQ=255)                  cgccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAG‑TATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                     <  1:496689/139‑1 (MQ=255)                                      ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                     >  2:249278/1‑120 (MQ=255)                                      ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                     <  1:249278/120‑1 (MQ=255)                                      ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGGCGATGCCTGAt                                                                                  >  1:858360/1‑139 (MQ=255)                                      ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGAt                                                                                  >  2:1016801/1‑139 (MQ=255)                                      ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGAt                                                                                  >  1:767479/1‑139 (MQ=255)                                      ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGAt                                                                                  >  2:601700/1‑139 (MQ=255)                                      ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGAt                                                                                  >  2:288412/1‑139 (MQ=255)                                           aaGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCAc                                                                                                    >  1:31745/1‑116 (MQ=255)                                           aaGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCAc                                                                                                    <  2:31745/116‑1 (MQ=255)                                                            gaTCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCa                                                            <  1:288412/139‑1 (MQ=255)                                                               cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTAt                                                              >  2:701337/1‑135 (MQ=255)                                                               cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTAt                                                              <  1:701337/135‑1 (MQ=255)                                                               cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCg                                                                                                                        >  1:850153/1‑76 (MQ=255)                                                               cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGc                                                                                                             >  2:466491/1‑87 (MQ=255)                                                               cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGc                                                                                                             <  1:466491/87‑1 (MQ=255)                                                               cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc                                                         >  2:127070/1‑139 (MQ=255)                                                               cTGACGACCATTGATGATCCTGCGTCATTGGATCAGAT‑CCGTCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCg                                                                                                                        <  2:850153/76‑1 (MQ=255)                                                                         ttGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCt                                               >  1:356388/1‑139 (MQ=255)                                                                            atgatCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGt                                                                                                               <  2:286613/72‑1 (MQ=255)                                                                            atgatCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGt                                                                                                               >  1:286613/1‑72 (MQ=255)                                                                             tgatCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAAc                                           <  1:601700/139‑1 (MQ=255)                                                                                tCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTcgcg                                                                                                                   >  1:614403/1‑64 (MQ=255)                                                                                tCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTcgcg                                                                                                                   <  2:614403/64‑1 (MQ=255)                                                                                tCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAcgcg                                                                    <  1:81841/111‑1 (MQ=255)                                                                                tCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAcgcg                                                                    >  2:81841/1‑111 (MQ=255)                                                                                 ccTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTg                                              <  1:537484/132‑1 (MQ=255)                                                                                 ccTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTg                                              >  2:537484/1‑132 (MQ=255)                                                                                 ccTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTa                                       >  2:384872/1‑139 (MQ=255)                                                                                 ccTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTa                                       >  1:974031/1‑139 (MQ=255)                                                                                  cTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAg                                      <  2:356388/139‑1 (MQ=255)                                                                                    gCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGt                                    <  2:245265/139‑1 (MQ=255)                                                                                     cgtcgtTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGTGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTc                                   >  2:1288933/1‑139 (MQ=255)                                                                                     cgtcgtTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCg                                                                                           <  2:318949/83‑1 (MQ=255)                                                                                     cgtcgtTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCg                                                                                           >  1:318949/1‑83 (MQ=255)                                                                                         gtTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGAt                               >  1:470960/1‑139 (MQ=255)                                                                                          ttGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                 >  1:907417/1‑88 (MQ=255)                                                                                          ttGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                 <  2:907417/88‑1 (MQ=255)                                                                                          ttGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTg                                                                         >  2:12833/1‑96 (MQ=255)                                                                                          ttGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTg                                                                         <  1:12833/96‑1 (MQ=255)                                                                                          ttGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa                                                      <  1:835211/115‑1 (MQ=255)                                                                                          ttGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa                                                      >  2:835211/1‑115 (MQ=255)                                                                                          ttGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGt                                    <  1:948364/133‑1 (MQ=255)                                                                                          ttGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGt                                    >  2:948364/1‑133 (MQ=255)                                                                                          ttGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATa                              <  2:767479/139‑1 (MQ=255)                                                                                          ttGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATa                              >  2:172272/1‑139 (MQ=255)                                                                                               tCAGAT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGgcg                         >  2:319546/1‑139 (MQ=255)                                                                                                   aT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATc                                                                                                          <  1:1126685/54‑1 (MQ=255)                                                                                                   aT‑CCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATc                                                                                                          >  2:1126685/1‑54 (MQ=255)                                                                                                        gCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGt                                        >  2:259550/1‑116 (MQ=255)                                                                                                        gCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGt                                        <  1:259550/116‑1 (MQ=255)                                                                                                         ccAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGt                                                                   <  2:388295/88‑1 (MQ=255)                                                                                                         ccAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGt                                                                   >  1:388295/1‑88 (MQ=255)                                                                                                         ccAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa                                                      >  2:554748/1‑101 (MQ=255)                                                                                                         ccAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa                                                      <  1:554748/101‑1 (MQ=255)                                                                                                         ccAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTc                      <  2:1289724/133‑1 (MQ=255)                                                                                                         ccAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTc                      >  1:1289724/1‑133 (MQ=255)                                                                                                         ccAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCg                >  1:557145/1‑139 (MQ=255)                                                                                                           aGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCa              <  2:974031/139‑1 (MQ=255)                                                                                                                 tGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGt                                        <  2:862309/107‑1 (MQ=255)                                                                                                                 tGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGt                                        >  1:862309/1‑107 (MQ=255)                                                                                                                 tGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGac        >  1:457283/1‑139 (MQ=255)                                                                                                                  ggAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGaca       <  1:172272/139‑1 (MQ=255)                                                                                                                  ggAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGaca       >  1:165465/1‑139 (MQ=255)                                                                                                                  ggAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGaca       >  1:968838/1‑139 (MQ=255)                                                                                                                       agTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATg  <  1:1016801/139‑1 (MQ=255)                                                                                                                           |                                                                                                                                       GATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGAT‑CCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATG  >  NC_000913/354471‑354726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑