Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,255,654	C→T	L271L (CTG→CTA)	plsB ←	glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,255,654	0	C	T	100.0%	180.9 / NA	56	L271L (CTG→CTA)	plsB	glycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/28);  total (28/28)

GAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCT  >  NC_000913/4255520‑4255783                                                                                                                                       |                                                                                                                                  gAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGaa                                                                                                                               <  2:660160/139‑1 (MQ=255)         cagGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGG‑CAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGgtcag                                                                                                                      <  2:763618/139‑1 (MQ=255)           ggTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGgtcagt                                                                                                                     <  1:300848/139‑1 (MQ=255)                tCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGc                                                                                                                <  2:925966/139‑1 (MQ=255)                tCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGc                                                                                                                <  2:724564/139‑1 (MQ=255)                  cATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGa                                                                                                              <  2:1260848/139‑1 (MQ=255)                    tgtgACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAat                                                                                                            <  2:1240833/139‑1 (MQ=255)                       gACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAatcat                                                                                                         >  1:608874/1‑139 (MQ=255)                              gTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTaa                                                                                                  >  1:525757/1‑139 (MQ=255)                                              aCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCaa                                                                                  <  2:742847/139‑1 (MQ=255)                                              aCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCaa                                                                                  <  2:50005/139‑1 (MQ=255)                                              aCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCaa                                                                                  <  2:431636/139‑1 (MQ=255)                                              aCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCaa                                                                                  <  2:383855/139‑1 (MQ=255)                                                cAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAtc                                                                                >  2:258857/1‑139 (MQ=255)                                                 aGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGgtcagtc                                                                                                                    >  1:499726/1‑102 (MQ=255)                                                 aGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGgtcagtc                                                                                                                    <  2:499726/102‑1 (MQ=255)                                                   cTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATctct                                                                             <  1:258857/139‑1 (MQ=255)                                                   cTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATctct                                                                             >  2:929361/1‑139 (MQ=255)                                                   cTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATctct                                                                             >  2:985995/1‑139 (MQ=255)                                                    tCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAatca                                                                                                          >  2:830331/1‑109 (MQ=255)                                                    tCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAatca                                                                                                          <  1:830331/109‑1 (MQ=255)                                                      aTGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTcc                                                                          <  2:608874/139‑1 (MQ=255)                                                      aTGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTcc                                                                          >  1:1003315/1‑139 (MQ=255)                                                       tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGgtcagtca                                                                                                                   >  1:1125532/1‑97 (MQ=255)                                                       tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGgtcagtca                                                                                                                   <  2:1125532/97‑1 (MQ=255)                                                       tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGgt                                                                                                                         >  1:1255845/1‑91 (MQ=255)                                                       tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGgt                                                                                                                         <  2:1255845/91‑1 (MQ=255)                                                        gACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCAt                                                                        >  1:819933/1‑139 (MQ=255)                                                                     cAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg                                                           <  2:908675/139‑1 (MQ=255)                                                                        cTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc                                                        >  1:814149/1‑139 (MQ=255)                                                                             gAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTctgctg                                                   >  2:628208/1‑139 (MQ=255)                                                                                     tCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttttt                                           >  1:1276326/1‑139 (MQ=255)                                                                                      cAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCaa                                                                                  >  2:1270361/1‑99 (MQ=255)                                                                                      cAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCaa                                                                                  <  1:1270361/99‑1 (MQ=255)                                                                                                     tGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAg                                                                     <  1:357721/97‑1 (MQ=255)                                                                                                     tGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAg                                                                     >  2:357721/1‑97 (MQ=255)                                                                                                     tGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg                                                           >  2:62465/1‑107 (MQ=255)                                                                                                     tGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg                                                           <  1:62465/107‑1 (MQ=255)                                                                                                      gATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTtg                          >  2:742419/1‑139 (MQ=255)                                                                                                            cTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTgcgc                    >  2:962581/1‑139 (MQ=255)                                                                                                             tGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTgcgcg                   >  2:495083/1‑139 (MQ=255)                                                                                                             tGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTgcgcg                   <  2:1276326/139‑1 (MQ=255)                                                                                                               gTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTg                                                                   <  1:826335/89‑1 (MQ=255)                                                                                                               gTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTg                                                                   >  2:826335/1‑89 (MQ=255)                                                                                                                  aaGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCa              <  1:929361/139‑1 (MQ=255)                                                                                                                     tCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCAtct           >  1:412439/1‑139 (MQ=255)                                                                                                                       ggTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCAtcttc         >  2:165839/1‑139 (MQ=255)                                                                                                                         ttCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAagag                                                                                               >  2:953678/1‑51 (MQ=255)                                                                                                                         ttCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAagag                                                                                               <  1:953678/51‑1 (MQ=255)                                                                                                                         ttCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCATTCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTa       >  1:268374/1‑139 (MQ=255)                                                                                                                         ttCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTctg                                                      <  2:628934/92‑1 (MQ=255)                                                                                                                         ttCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTctg                                                      >  1:628934/1‑92 (MQ=255)                                                                                                                         ttCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTtgct                        <  1:519107/122‑1 (MQ=255)                                                                                                                         ttCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTtgct                        >  2:519107/1‑122 (MQ=255)                                                                                                                             aGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGc   <  2:814149/139‑1 (MQ=255)                                                                                                                              ggTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCt  >  2:643088/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                  GAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCT  >  NC_000913/4255520‑4255783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑