| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 371,542 | C→T | Q102* (CAA→TAA) | mhpC → | 2‑hydroxy‑6‑ketonona‑2,4‑dienedioic acid hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 371,542 | 0 | C | T | 92.6% | 77.9 / ‑0.0 | 27 | coding (304/867 nt) | mhpC | 2‑hydroxy‑6‑ketonona‑2,4‑dienedioic acid hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (14/11); minor base . (1/1); total (15/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.18e-01 | |||||||||||
CCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAG > NC_000913/371416‑371672 | ccGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCg < 1:1005697/139‑1 (MQ=255) ggTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCaaa > 1:1207895/1‑139 (MQ=255) ccTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATg < 1:1301280/139‑1 (MQ=255) ctgctgGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATgg > 1:721369/1‑139 (MQ=255) tgGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCAc > 2:842471/1‑116 (MQ=255) tgGATTGTCCGCGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCAc < 1:842471/116‑1 (MQ=255) ggCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATcc < 2:962522/97‑1 (MQ=255) ggCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATcc > 1:962522/1‑97 (MQ=255) gTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGt > 2:733377/1‑139 (MQ=255) tGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGAT‑AACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGtt > 1:546522/1‑103 (MQ=255) tGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGAT‑AACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGtt < 2:546522/103‑1 (MQ=255) gTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCgg < 1:1293273/139‑1 (MQ=255) cgcgATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGc > 1:106532/1‑58 (MQ=255) cgcgATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGc < 2:106532/58‑1 (MQ=255) cgATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGtt > 1:436145/1‑107 (MQ=255) cgATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGtt < 2:436145/107‑1 (MQ=255) aCGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATcc > 1:285417/1‑47 (MQ=255) aCGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATcc < 2:285417/47‑1 (MQ=255) aCGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGggcggc > 1:1011255/1‑139 (MQ=255) aCGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACCGGTGCTGATGggcggc > 1:618502/1‑139 (MQ=255) gAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGcggcgg > 1:1059438/1‑139 (MQ=255) aaTCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGtt > 2:1103543/1‑87 (MQ=255) aaTCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGtt < 1:1103543/87‑1 (MQ=255) aGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTGGTTGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGGGCGGGTCGGCAAACTGTTGTTGATGGGCGGCGGTACGGGCGGCa < 2:630003/139‑1 (MQ=255) tggtggATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAg > 1:871084/1‑139 (MQ=255) tggATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATa < 2:1301/57‑1 (MQ=255) tggATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATa > 1:1301/1‑57 (MQ=255) | CCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAG > NC_000913/371416‑371672 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |