Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	382,579	G→A	*302* (TAG→TAA)	insD1 →	IS2 transposase TnpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	382,579	0	G	A	100.0%	168.9 / NA	53	*302* (TAG→TAA)	insD1	IS2 transposase TnpB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/28);  total (25/28)

GCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACC  >  NC_000913/382460‑382705                                                                                                                        |                                                                                                                               gCGTTCGAGCGTCATAACGAATGGCATCTGCATAGTCAGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCAGGCTTGTAATGGGTTAAGTGATACCAAATGTCTGCAAATATAAGGGCACATCCAATTACCTa                                                                                                             >  2:938502/1‑139 (MQ=11)      cGAGCATTATAACGAATTGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAgg                                                                                                        <  2:845671/139‑1 (MQ=255)               taACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTgcg                                                                                               <  1:949290/139‑1 (MQ=255)                  cGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCa                                                                                            <  2:143490/139‑1 (MQ=255)                    aaTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAAt                                                                                          <  2:543605/139‑1 (MQ=255)                    aaTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAAt                                                                                          <  1:39389/139‑1 (MQ=255)                      tGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCa                                                                                        <  2:1186850/139‑1 (MQ=255)                           tCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTAcc                                                                                   >  1:851977/1‑139 (MQ=255)                            ccGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCg                                                                                  <  1:1029934/139‑1 (MQ=255)                                aTAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGAcc                                                                              <  2:851977/139‑1 (MQ=255)                                  aGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGAcc                                                                              >  2:16727/1‑137 (MQ=255)                                  aGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGAcc                                                                              <  1:16727/137‑1 (MQ=255)                                    tGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGa                                                                                >  2:921315/1‑133 (MQ=255)                                    tGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGa                                                                                <  1:921315/133‑1 (MQ=255)                                     gcgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCt                                                                                                              >  2:132620/1‑102 (MQ=25)                                     gcgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCt                                                                                                              <  1:132620/102‑1 (MQ=25)                                      cgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCTATCACTACCGGACCCTGAAt                                                                        >  2:865856/1‑139 (MQ=255)                                            gTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGTCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGACAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGc                                                                  <  1:35784/139‑1 (MQ=255)                                              tATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCAc                                                                >  2:857095/1‑139 (MQ=255)                                                    tcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGAc                                                          <  2:946120/139‑1 (MQ=255)                                                       ccACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACaaa                                                       >  1:19557/1‑139 (MQ=255)                                                       ccACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACaaa                                                       >  2:8756/1‑139 (MQ=255)                                                        cACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAAt                                                      <  1:351170/139‑1 (MQ=255)                                                                tatCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAAt                                                                                          >  1:717211/1‑95 (MQ=255)                                                                tatCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAAt                                                                                          <  2:717211/95‑1 (MQ=255)                                                                     gCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTcgcg                                         <  1:8756/139‑1 (MQ=255)                                                                     gCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTcgcg                                         >  1:30985/1‑139 (MQ=255)                                                                            cGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGa                                                                                <  2:1035437/93‑1 (MQ=255)                                                                            cGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGa                                                                                >  1:1035437/1‑93 (MQ=255)                                                                                         ggTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGc                                                                  >  2:290767/1‑94 (MQ=255)                                                                                         ggTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGc                                                                  <  1:290767/94‑1 (MQ=255)                                                                                             aaGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAAt                                                                                          >  2:987594/1‑66 (MQ=255)                                                                                             aaGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAAt                                                                                          <  1:987594/66‑1 (MQ=255)                                                                                              aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCa                                                                                        <  2:1072236/67‑1 (MQ=255)                                                                                              aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCa                                                                                        >  1:1072236/1‑67 (MQ=255)                                                                                                 gATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCa                                                                                        >  2:942524/1‑64 (MQ=255)                                                                                                 gATAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCa                                                                                        <  1:942524/64‑1 (MQ=255)                                                                                                   tAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGt                                                                                                    >  1:1122246/1‑50 (MQ=255)                                                                                                   tAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGt                                                                                                    <  2:1122246/50‑1 (MQ=255)                                                                                                   tAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTgcg                                                                                               <  2:738169/55‑1 (MQ=255)                                                                                                   tAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTgcg                                                                                               >  1:738169/1‑55 (MQ=255)                                                                                                   tAACAGATGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTtcatca           >  1:274275/1‑139 (MQ=255)                                                                                                          tGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCa                                                                                            >  2:1019605/1‑51 (MQ=255)                                                                                                          tGTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCa                                                                                            <  1:1019605/51‑1 (MQ=255)                                                                                                           gTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGtt                 >  2:296971/1‑125 (MQ=255)                                                                                                           gTCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGtt                 <  1:296971/125‑1 (MQ=255)                                                                                                            tCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCg                                                                                  <  2:34831/59‑1 (MQ=255)                                                                                                            tCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCg                                                                                  >  1:34831/1‑59 (MQ=255)                                                                                                            tCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTg                                                                           >  1:277944/1‑66 (MQ=255)                                                                                                            tCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTg                                                                           <  2:277944/66‑1 (MQ=255)                                                                                                            tCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCCACTGCAGTTtcat             <  2:1036895/128‑1 (MQ=255)                                                                                                            tCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTAcc  >  2:588821/1‑139 (MQ=255)                                                                                                            tCTGGAAATATAAGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACAATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTtcat             >  1:1036895/1‑128 (MQ=255)                                                                                                                        |                                                                                                                               GCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACC  >  NC_000913/382460‑382705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑