Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,623,101	C→T	*339* (TAG→TAA)	lplA ←	lipoate‑protein ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,623,101	0	C	T	100.0%	58.1 / NA	20	*339* (TAG→TAA)	lplA	lipoate‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/10);  total (10/10)

ATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTG  >  NC_000913/4622966‑4623223                                                                                                                                        |                                                                                                                           aTCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTAc                                                                                                                         >  1:770496/1‑139 (MQ=255)                         cAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGcc                                                                                                 <  2:770496/139‑1 (MQ=255)                            aCGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGAt                                                                                              >  2:9294/1‑139 (MQ=255)                             cGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATa                                                                                             >  1:1228886/1‑139 (MQ=255)                             cGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATa                                                                                             <  2:1228886/139‑1 (MQ=255)                                  gCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGccatc                                                                                                       <  1:15772/124‑1 (MQ=255)                                  gCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGccatc                                                                                                       >  2:15772/1‑124 (MQ=255)                                                        aGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACGGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCg                                                                  >  1:139815/1‑139 (MQ=255)                                                               tCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTc                                                           >  2:81547/1‑139 (MQ=255)                                                                       ttGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACa                                                   <  1:437721/139‑1 (MQ=255)                                                                                aaaTGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGc                                          <  1:9294/139‑1 (MQ=255)                                                                                                  gtcAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTATCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCatat                        <  1:190402/137‑1 (MQ=255)                                                                                                       aTCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACGGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTgcg                   <  2:139815/139‑1 (MQ=255)                                                                                                       aTCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGAACTCCTGTTGCAGCATATCTgcg                   <  2:337703/139‑1 (MQ=255)                                                                                                             gagaAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTg                                <  1:833030/120‑1 (MQ=255)                                                                                                             gagaAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTg                                >  2:833030/1‑120 (MQ=255)                                                                                                                aaaGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAgg          >  1:185439/1‑139 (MQ=255)                                                                                                                  aGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCa        >  2:78932/1‑139 (MQ=255)                                                                                                                     tGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGcc     >  2:197826/1‑139 (MQ=255)                                                                                                                        ccGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGCAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTCGCAGCATAACTGCGCGGTACAGGCAGCCTTg  <  2:843122/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                           ATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTG  >  NC_000913/4622966‑4623223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑